A5101626349- Ultrasound and Hyperthermia Applications
- Nanoplatforms for cancer theranostics
- Photoacoustic and Ultrasonic Imaging
- Nanoparticle-Based Drug Delivery
- Ultrasound and Cavitation Phenomena
- Cancer, Hypoxia, and Metabolism
- Electrospun Nanofibers in Biomedical Applications
- Ultrasound Imaging and Elastography
- Inflammasome and immune disorders
- Extracellular vesicles in disease
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- RNA Interference and Gene Delivery
- Autophagy in Disease and Therapy
- Immunotherapy and Immune Responses
- Minerals Flotation and Separation Techniques
- Neurological Disease Mechanisms and Treatments
- Nitric Oxide and Endothelin Effects
- Heme Oxygenase-1 and Carbon Monoxide
- Corporate Insolvency and Governance
- Metabolomics and Mass Spectrometry Studies
- Cancer, Lipids, and Metabolism
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Curcumin's Biomedical Applications
- Lipid metabolism and biosynthesis
Shandong Provincial Hospital
2024
Shandong First Medical University
2024
Qilu Hospital of Shandong University
2015-2024
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2017-2020
Henan University
2017-2020
Wenzhou Medical University
2011
Women’s Environmental Institute
2001
Taipei Municipal Jen-Ai Hospital
1996
Immunotherapy had demonstrated inspiring effects in tumor treatment, but only a minority of people could benefit owing to the hypoxic and immune-suppressed microenvironment (TME). Therefore, there was an urgent need for strategy that relieve hypoxia increase infiltration lymphocytes simultaneously. In this study, novel acidity-responsive nanoscale ultrasound contrast agent (L-Arg@PTX nanodroplets) constructed co-deliver paclitaxel (PTX) L-arginine (L-Arg) using homogenization/emulsification...
Ultrasound-targeted delivery of nanobubbles (NBs) has become a promising strategy for noninvasive drug delivery. The biosafety and drug-transporting ability NBs have been research hotspot, especially regarding chitosan due to their biocompatibility high biosafety. Since the drug-carrying capacity performance ultrasound-assisted remain unclear, aim this study was synthesize doxorubicin hydrochloride (DOX)-loaded biocompatible assess capacity. In study, size distribution measured by dynamic...
The inhibition of autophagy is a feasible clinical strategy in tumor therapy. Traditional inhibitors are limited therapy due to nonspecific biodistribution, systemic toxicity and antitumor effect. Herein, the inhibitor hydroxychloroquine (HCQ)-loaded nanodroplets (NDs) synthesized overcome these drawbacks. HCQ-NDs endowed with endogenous pH- exogenous ultrasound-responsive drug release contrast enhanced ultrasound imaging performance. combined application ultrasound-targeted microbubble...
Aim: O-carboxymethyl chitosan/perfluorohexane nanodroplets (O-CS NDs) and doxorubicin-loading chitosan were synthesized functionally tested as drug delivery system in vitro. Materials & methods: The characteristics, charge conversion, stability, cytotoxicity, ultrasound imaging ability, interaction with tumor cells of the eradication on doxorubicin-loaded investigated. Results: O-CS NDs (below 200 nm) achieved higher cellular associations at acidic pH, great serum pH-dependent conversion...
Neddylation is a ubiquitination-like pathway that controls cell survival and proliferation by covalently conjugating NEDD8 to lysines in specific substrate proteins. However, the physiological role of neddylation mammalian metabolism remains elusive, no mitochondrial targets have been identified. Here, we report mouse models with liver-specific deficiency or ubiquitin-like modifier activating enzyme 3 (UBA3), catalytic subunit NEDD8-activating enzyme, exhibit neonatal death spontaneous fatty...
Abstract As a potential drug/gene delivery system, the ultrasound-targeted microbubble destruction (UTMD) system can be used as vehicle well increasing permeability of biological barriers to enhance effect tumor treatment. However, UTMD in EMT process is unknown. In this study, we aimed investigate and mechanism induced oxidative stress inhibiting breast cancer. Human MDA231 cells were treated with (MB), ultrasound (US) UTMD, respectively. The generation stress, levels miR-200c, ZEB1...
Multiple drug resistance (MDR) exists in divergent cancers including triple negative breast cancer (TNBC) and partly results the to many first-line anti-cancer agents, bringing a big challenge TNBC management. To develop novel therapeutics, our study, hyaluronic acid (HA)–carboxymethyl chitosan (CMC) conjugate linked via disulfide-bond (HA-SS-CMC, HSC) was synthesized fabricate nanodroplets (NDs). The NDs encapsulating doxorubicin (DOX) perfluorohexane (DOX-HSC-NDs) were prepared...
Abstract Pyroptosis, a novel type of programmed cell death (PCD), which provides feasible therapeutic option for the treatment tumors. However, due to hypermethylation promoter, critical protein Gasdermin E (GSDME) is lacking in majority cancer cells, cannot start pyroptosis process and leads dissatisfactory effects. Additionally, quick clearance, systemic side effects, low concentration at tumor site conventional reagents restrict their use clinical therapy. Here, we described combination...
Synergistic chemoradiotherapy (CRT) has become a primary effective curative approach for many solid cancers. However, CRT is still associated with several obstacles, including the increases in side effects and systemic toxicity. Incorporating nanocarriers into new exciting to solve these obstacles. The purpose of present study was design unique pH- ultrasound-responsive perfluoropentane-encapsulated, paclitaxel (PTX)-loaded carboxymethyl chitosan nanodroplets (NDs) combined imaging...
The construction of multifunctional oncotherapy nanoplatforms that combine diagnosis and treatment remains challenging. Nanodroplets (NDs), which simultaneously enhance ultrasound imaging therapeutic effects, are a potential strategy for non-invasive drug delivery. To achieve the goals precise medicine, novel SP94 peptide-modified doxorubicin-loaded ultrasonic NDs (SP94-DOX-NDs) castration-resistant prostate cancer (CRPC) targeting were constructed in this study. characteristics,...
Triple-negative breast cancer (TNBC) is known to be the most aggressive form of cancer. Due its high recurrence and mortality rates, treatment TNBC a significant challenge for medical community. Besides, ferroptosis an emerging regulatory cell death that may provide new insights into TNBC. As central inhibitor process, selenoenzyme glutathione peroxidase 4 (GPX4) classical therapeutic target. However, inhibition GPX4 expression quite detrimental normal tissues. Ultrasound contrast agents, as...
Despite rapid advances in metabolic therapies over the past decade, their efficacy melanoma has been modest, largely due to interaction between cancer-associated fibroblasts (CAFs) and cancer cells promote growth. Altering tumor microenvironment (TME) is challenging elusive. CAFs critical for glutamine deprivation survival melanoma. In this research, we assembled a CAFs-targeted, controlled-release nanodroplets combined delivery of amino acid transporter ASCT2 (SLC1A5) inhibitor V9302...
Abstract Endoplasmic reticulum (ER) stress‐induced cancer cell apoptosis has become a novel signaling target for the development of therapeutic drugs treatment. Curcumin, dietary phytochemical, exhibits growth‐suppressive activity against cells via multitarget mechanisms. However, low stability and poor pharmacokinetics significantly limit its clinical applications. Thus, we designed synthesized monocarbonyl analog curcumin, 1,5‐bis(2‐methoxyphenyl) penta‐1,4‐dien‐3‐one (B63). This compound...
As an injectable anticancer drug delivery system, the biological safety of nanocarriers is most important prerequisite for their clinical application. The objective our study was to synthesize special ultrasound-responsive highly biocompatible chitosan nanodroplets (BCNDs), observe spatiotemporally control doxorubicin (DOX) in vivo. experimental results showed that BCNDs were successfully prepared with high biosafety vivo and great ultrasound imaging ability. DOX-BCNDs promoted effects DOX...
To synthesize and evaluate a novel folate-conjugated ultrasonic nanobubble (HA-FOL-NB) loading low-molecular-weight hyaluronic acid (LMW-HA) for specific tumor-associated macrophages (TAMs) targeting reeducation.The characteristics, cytotoxicity, contrast-enhanced ultrasound imaging (CEUS), ability to TAMs of HA-FOL-NBs were investigated. The reprogramming function combining targeted destruction was assessed as well.HA-FOL-NBs (about 342 nm) showed remarkable contrast enhancement images,...
Ultrasound nanodroplets (NDs) have been reported as a promising nanocarrier for siRNA delivery depending on its unique strengths of sonoporation. Presently, common means NDs-mediated is through electrostatic interaction, but challenges like cationic toxicity still exist. In this study, we demonstrated novel strategy to construct negatively charged and ultrasound (US)-responsive O-carboxymethyl chitosan (O-CMS) NDs targeted system three-way junction bacteriophage phi29 DNA packaging motor...
Aim: To synthesize and evaluate a novel FH peptide-modified ultrasonic nanobubble-loading doxorubicin (FH-NB-DOX) for specially cancer-associated fibroblasts (CAFs) targeting eradication. Materials & methods: The characteristics, cytotoxicity, contrast-enhanced ultrasound imaging (CEUI), ability eradicating CAFs of these NBs were investigated. Results: FH-NB-DOX (about 208 nm) showed good CEUI, achieved higher due to the conjunction peptide tenascin C protein high-level expressed in CAFs....
To synthesize echogenic chitosan/perfluorohexane nanodroplets (CNDs) for DKK-2 gene delivering in a spatiotemporally controlled manner vitro.The characteristics, contrast-enhanced ultrasound imaging, DNA binding and DNase protection capacity, transfection effects on LNCaP cells of these CNDs were investigated.The obtained showed positive surface charges could attract the genetic cargo with negative to form nanocomplexes. Agarose gel electrophoresis confirmed pDNA. pDNA-loaded CNDs,...
Abstract Nanosized drug delivery systems have rapidly emerged as a promising approach to tumor therapy, which still many challenges in clinical application. In this study, doxorubicin-loaded O-carboxymethyl chitosan/perfluorohexane nanodroplets (O-CS-DOX NDs) were synthesized and functionally tested an effective system vitro vivo. O-CS-DOX NDs with small size (159.6 nm) good doxorubicin encapsuling ability showed pH- ultrasound-dependent release profile satisfying ultrasound imaging...
BACKGROUND:Ultrasound/microbubble (USMB)-mediated sonoporation is a new strategy with minimal procedural invasiveness for targeted and site-specific drug delivery to tumors. The purpose of this study was explore the effect different breast cancer cell lines on efficiency, then identify an optimal combination USMB parameters maximize efficiency each tumor line. MATERIAL AND METHODS:Three drug-sensitive – MCF-7, MDA-MB-231, MDA-MB-468 1 multidrug resistance (MDR) line MCF-7/ADR were chosen. An...