A5019909422- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Biosensors and Analytical Detection
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
- Semantic Web and Ontologies
- Advanced Biosensing Techniques and Applications
- Effects and risks of endocrine disrupting chemicals
- Molecular Sensors and Ion Detection
- Plasmonic and Surface Plasmon Research
- Gut microbiota and health
- Multiple Myeloma Research and Treatments
- Gold and Silver Nanoparticles Synthesis and Applications
- Urban Green Space and Health
- Nanoparticle-Based Drug Delivery
- Topic Modeling
- SARS-CoV-2 detection and testing
- Molecular Junctions and Nanostructures
- Indoor Air Quality and Microbial Exposure
- Electrochemical Analysis and Applications
- Natural Language Processing Techniques
- Nanopore and Nanochannel Transport Studies
- Protein Degradation and Inhibitors
- Peptidase Inhibition and Analysis
Anhui Medical University
2024-2025
Anhui Provincial Hospital
2024
Lenovo (China)
2024
Nanjing University
2021-2023
Xijing University
2021-2022
Capital Normal University
2017-2021
Rheumatoid arthritis (RA) severely threatens human health by causing inflammation, swelling, and pain in the joints resulting persistent synovitis irreversible joint disability. In development of RA, pro-inflammatory M1 macrophages, which express high levels reactive oxygen species (ROS) nitric oxide (NO), induce synovial inflammation bone erosion. Eliminating ROS NO inflamed is a potential RA therapeutic approach, can drive transition macrophages to anti-inflammatory M2 phenotype. Taking...
Triple-negative breast cancer (TNBC) is highly aggressive with a poor clinical prognosis and no targeted therapy. The c-Myc protein master transcription factor potential therapeutic target for TNBC. In this study, we develop PROTAC (PROteolysis TArgeting Chimera) based on TNA (threose nucleic acid) DNA that effectively targets degrades c-Myc. aptamer selected in vitro to bind the c-Myc/Max heterodimer appended E-box sequence create high-affinity, biologically stable bivalent binder. TNA-E...
Phthalic acid esters (PAEs) are ubiquitous in the environment, and some of them recognized as endocrine disruptors that cause concerns on ecosystem functioning public health. Due to diversity PAEs there is a vital need detect total concentration timely low-cost way. To fulfill this requirement, it highly desired obtain group-specific PAE binders specific basic skeleton. In study, for first time we have identified PAE-binding aptamers via rationally designed target immobilization. The two...
Chemoimmunotherapy represents an attractive approach for cancer treatment, which requires a compatible co-delivery system to achieve the synergetic therapeutic effects. Among various nanocarriers, DNA nanostructures have recently been proposed as promising candidates drug delivery due excellent biocompatibility. Herein, we employ tetrahedron create nanoplatform that combines chemotherapeutic doxorubicin and immunotherapeutic agent CpG oligodeoxynucleotides. The DTN-CpG/DOX nanoparticles show...
Exosomes are nanoscale membrane vesicles actively released by cells and play an important role in the diagnosis of cancer-related diseases. However, it is challenging to efficiently enrich exosomes from extracellular fluids. In this work, we used DNA nanostructures as "nanoweights" during centrifugation facilitate enrichment cancerous human serum. Two different tetrahedral (DTNs), each carrying a specific aptamer for exosome biomarker recognition, were incubated with clinical samples...
Poor antimatrix interference capability of aptamers is one the major obstacles preventing their wide applications for real-sample detections. Here, we devise a multiple-function interface, denoted as nanoscale affinity double layer (NADL), to overcome this bottleneck via in situ simultaneous target enrichment, purification, and detection. The NADL consists an upper aptamer purification sensing lower solid-phase microextraction (SPME) sample enrichment. targets flowing through...
The aim of this research was to examine the correlation between exposure bisphenol analogues (BPs), such as A (BPA), F (BPF), and S (BPS), risk developing systemic lupus erythematosus (SLE). Ultra performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) utilized measure levels BPA, BPF, BPS in urine 168 female participants diagnosed with SLE 175 who were deemed healthy controls. Logistic regression models assess connections SLE. findings indicated that BPA BPF individuals...
The manufacture of DNA origami nanostructures with highly ordered functional motifs is great significance for biomedical applications. Here, we present a robust strategy to produce customized scaffolds integrated aptamer sequences, which enables direct construction structures. As demonstrated, aptamers various numbers and types were efficiently stably in user-defined positions the scaffolds. Specifically, two different thrombin sequences simultaneously inserted into M13mp18 phage genome....
We report the use of a protein ligase to covalently ligate peptide nucleic acid (PNA). The rapid ligation demands only an N-terminal GL dipeptide in target and C-terminal NGL tripeptide PNA. demonstrate versatility this approach by attaching PNA strand three different proteins. Lastly, we show that erasable imaging EGFR on HEK293 cell membranes is achieved with DNA origami nanostructures toehold-mediated displacement.
Phthalic acid esters (PAEs) areone of the major groups persistent organic pollutants. The group-specific detection PAEs is highly desired due to rapid growing congeners. DNA aptamers have been increasingly applied as recognition elements on biosensor platforms, but selecting toward hydrophobic small molecule targets, such PAEs, rarely reported. This work describes a bead-based method designed select PAEs. amino group functionalized dibutyl phthalate (DBP-NH2) anchor target was synthesized...
Phthalic acid esters (PAEs) areone of the major groups persistent organic pollutants. The group-specific detection PAEs is highly desired due to rapid growing congeners. DNA aptamers have been increasingly applied as recognition elements on biosensor platforms, but selecting toward hydrophobic small molecule targets, such PAEs, rarely reported. This work describes a bead-based method designed select PAEs. amino group functionalized dibutyl phthalate (DBP-NH2) anchor target was synthesized...
Abstract High levels of reactive oxygen species (ROS) and nitric oxide (NO) generated by M1 macrophages induce inflammation in the development rheumatoid arthritis (RA). The eliminating ROS NO therefore represents an alternative strategy for RA treatment. Because DNA molecules possess ROS- endogenous NO-scavenging capability, herein, we develop a nanomedicine based on triangular origami nanostructures targeted We showed that folic acid-modified (FA-tDONs) could reduce simultaneously inside...
Abstract Methods to efficiently and site-specifically conjugate proteins nucleic acids could enable exciting application in bioanalytics biotechnology. Here, we report the use of strict protein ligase covalently ligate a peptide acid (PNA). The rapid ligation requires only short N-terminal GL dipeptide target C-terminal NGL tripeptide PNA. We demonstrate versatility this approach by conjugating three different types with PNA strand. biostable strand then serves as generic landing platform...
Abstract Exosomes are nanoscale membrane vesicles actively released by cells and play an important role in the diagnosis of cancer-related diseases. However, it is challenging to efficiently enrich exosomes from extracellular fluids. In this work, we used DNA-tetrahedron as a nanoweight during centrifugation precisely tumor complex biological environment. Two different DNA tetrahedral nanostructures (DTAs), each carrying specific aptamer for exosome biomarker recognition, were incubated with...