A5024323959- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Extracellular vesicles in disease
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Mechanical and Optical Resonators
- Analytical Chemistry and Sensors
- Gold and Silver Nanoparticles Synthesis and Applications
- Immunotherapy and Immune Responses
- Quantum Dots Synthesis And Properties
- Bacterial Genetics and Biotechnology
- Biosensors and Analytical Detection
- Nanoplatforms for cancer theranostics
- Plasmonic and Surface Plasmon Research
- Acoustic Wave Resonator Technologies
- Phagocytosis and Immune Regulation
- Layered Double Hydroxides Synthesis and Applications
- Asymmetric Synthesis and Catalysis
- Atherosclerosis and Cardiovascular Diseases
- Neuroscience and Neural Engineering
Nanjing University
2010-2025
Applied Science Private University
2025
State Key Laboratory of Analytical Chemistry for Life Science
2022-2025
Collaborative Innovation Center of Advanced Microstructures
2017-2024
Hankou University
2019-2024
Zhejiang A & F University
2024
Southern University of Science and Technology
2024
Xijing University
2021-2022
University of California, San Diego
2016-2019
Yale University
2014-2015
Rheumatoid arthritis (RA) severely threatens human health by causing inflammation, swelling, and pain in the joints resulting persistent synovitis irreversible joint disability. In development of RA, pro-inflammatory M1 macrophages, which express high levels reactive oxygen species (ROS) nitric oxide (NO), induce synovial inflammation bone erosion. Eliminating ROS NO inflamed is a potential RA therapeutic approach, can drive transition macrophages to anti-inflammatory M2 phenotype. Taking...
Triple-negative breast cancer (TNBC) is highly aggressive with a poor clinical prognosis and no targeted therapy. The c-Myc protein master transcription factor potential therapeutic target for TNBC. In this study, we develop PROTAC (PROteolysis TArgeting Chimera) based on TNA (threose nucleic acid) DNA that effectively targets degrades c-Myc. aptamer selected in vitro to bind the c-Myc/Max heterodimer appended E-box sequence create high-affinity, biologically stable bivalent binder. TNA-E...
Lead halide perovskites with well-defined morphology have attracted attention for their unique properties as a promising new class of semiconductor materials in photovoltaics and optoelectronics. However, controlling morphologies compositions perovskite nanostructures improved stability, especially double cation lead perovskite, still remains challenge. Here, we demonstrate colloidal synthetic approach direct synthesis stable single-crystal formamidinium (FA) cesium FA0.33Cs0.67PbBr3–xIx (0...
Functional DNAs are valuable molecular tools in chemical biology and analytical chemistry but suffer from low activities due to their limited functionalities. Here, we present a chemoenzymatic method for site-specific installation of diverse functional groups on DNA, showcase the application this enhance catalytic activity DNA catalyst. Through introduction distinct groups, such as hydroxyl, carboxyl, benzyl, at specific positions, achieve significant enhancements RNA-cleaving deoxyribozyme...
<italic>In vitro</italic> selected threose nucleic acid (TNA) aptamers blocked PD-1/PD-L1 interaction and significantly inhibited tumour growth in xenograft mouse model.
Abstract Inflammatory bowel disease (IBD) encompasses a collection of chronic inflammatory conditions impacting the gastrointestinal tract, with discernible global rise in prevalence and severity. The overabundance reactive oxygen species (ROS) intestinal environment leads disruption local redox homeostasis, perturbation gut microbiota, exacerbating IBD. Consequently, mitigating excess ROS preserving microbiota homeostasis have emerged as effective approaches for IBD management. In this...
Threose nucleic acid (TNA) has been considered a potential RNA progenitor in evolution due to its chemical simplicity and base pairing property. Catalytic TNA sequences with ligase activities might have facilitated the transition world. Here we report isolation of enzymes by vitro selection. The identified enzyme T8-6 catalyzes formation 2′–5′ phosphoester bond between 2′,3′-diol 5′-triphosphate group, kobs 1.1 × 10–2 min–1 (40 mM Mg2+, pH 9.0). For efficient reaction, requires UA|GA at...
Abstract Background The recent development of dendritic cell (DC)‐based immunotherapy has resulted in advances glioblastoma multiforme (GBM) treatment. However, the fate DCs GBM microenvironment, especially microenvironments which glioma stem (GSCs)‐mediated remodeling highly immunosuppressive conditions, not yet been fully investigated. Methods Observed interaction between GSCs and primary cultured a dual‐color tracing model, monoclonal continuously passaged proliferative DCs, named...
Given the multifactorial pathogenesis of atherosclerosis (AS), a chronic inflammatory disease, combination therapy arises as compelling approach to effectively address complex interplay pathogenic mechanisms for more desired treatment outcome. Here, we present
Extracellular vesicles (EVs) play a crucial role as important mediators of intercellular communication in the progression tumors. The capture and analysis tumor-derived EVs offer new possibilities for application cancer liquid biopsies. This study aims to construct DNA tetrahedral nanostructure that specifically recognizes HER2 CD63, enabling effective enrichment detection HER2-expressing (HEVs). We enriched HEVs from cell lines 13 random clinical samples validated their characteristics by...
As a milestone in DNA self-assembly, origami has demonstrated powerful applications many fields. However, the scarce availability of long single-stranded (ssDNA) limits size and sequences nanostructures, which turn impedes further development. In this study, we present robust strategy to produce circular ssDNA scaffold strands with custom-tailored lengths sequences. These products were then used as scaffolds for constructing various nanostructures. This scalable method produces at low cost...
Chemoimmunotherapy represents an attractive approach for cancer treatment, which requires a compatible co-delivery system to achieve the synergetic therapeutic effects. Among various nanocarriers, DNA nanostructures have recently been proposed as promising candidates drug delivery due excellent biocompatibility. Herein, we employ tetrahedron create nanoplatform that combines chemotherapeutic doxorubicin and immunotherapeutic agent CpG oligodeoxynucleotides. The DTN-CpG/DOX nanoparticles show...
Exosomes are nanoscale membrane vesicles actively released by cells and play an important role in the diagnosis of cancer-related diseases. However, it is challenging to efficiently enrich exosomes from extracellular fluids. In this work, we used DNA nanostructures as "nanoweights" during centrifugation facilitate enrichment cancerous human serum. Two different tetrahedral (DTNs), each carrying a specific aptamer for exosome biomarker recognition, were incubated with clinical samples...
Selective protein recognition is critical in molecular biology techniques such as Western blotting and ELISA. Successful detection of the target proteins these methods relies on specific interaction antibody, which often brings high production cost requires long incubation time. Aptamers represent an alternative class simple affordable affinity reagents for recognition. And replacing antibodies with aptamers would potentially be more time- cost-effective. In this work, multiple fluorescent...
DNA particles are programmed to assemble with precision into complex lattices
A metal–organic framework with alkene-functional groups was constructed and postsynthetically modified Ag( i ) for antibacterial applications.
Functional nucleic acids including aptamers and DNAzymes are a class of valuable molecular tool for biotechnology. However, DNA RNA catalysts suffer from low biological stability limited chemical diversity. Xeno-nucleic (XNAs) refer to acid analogues containing sugar moieties that structurally distinct possess advantageous properties. In this article, we first focus on two types XNAs, threose (TNA) 2'-fluoroarabinose (FANA), summarize recent in vitro selections TNA FANA catalysts. We then...
One of the key advantages using Drosophila melanogaster as a genetic model organism is ability to conduct saturation mutagenesis screens identify genes and pathways underlying given phenotype. Despite large number tools developed facilitate downstream cloning mutations obtained from such screens, current procedure remains labor intensive, time consuming, costly. To address this issue, we designed an efficient strategy for rapid identification heterozygous in fly genome by combining rough...
The manufacture of DNA origami nanostructures with highly ordered functional motifs is great significance for biomedical applications. Here, we present a robust strategy to produce customized scaffolds integrated aptamer sequences, which enables direct construction structures. As demonstrated, aptamers various numbers and types were efficiently stably in user-defined positions the scaffolds. Specifically, two different thrombin sequences simultaneously inserted into M13mp18 phage genome....
DNA nanostructures possess unique programmability and addressability exhibit a wide variety of potential applications. Recently, they demonstrated their ability to be ideal carriers antibacterial drugs. In this study, the first use six-helix bundle (6HB) nanostructure co-deliver antisense oligonucleotide (ASO) silver ions is reported. Although 6HB with Ag+ shows excellent effect against both Gram-negative Escherichia coli Gram-positive Staphylococcus aureus, ASO selectively inhibits S....