A5057273967- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Cancer-related molecular mechanisms research
- Hepatocellular Carcinoma Treatment and Prognosis
- Advanced biosensing and bioanalysis techniques
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- RNA modifications and cancer
- Metabolism, Diabetes, and Cancer
- Cancer Research and Treatments
- Cancer Immunotherapy and Biomarkers
- Cancer-related gene regulation
- Lung Cancer Research Studies
- Mesenchymal stem cell research
- Liver Disease Diagnosis and Treatment
- Circular RNAs in diseases
- Cancer Research and Treatment
- Pancreatic and Hepatic Oncology Research
- Nanoparticle-Based Drug Delivery
- Dendrimers and Hyperbranched Polymers
- Viral-associated cancers and disorders
- Global Cancer Incidence and Screening
- Cancer Treatment and Pharmacology
- Wnt/β-catenin signaling in development and cancer
Nanjing University of Chinese Medicine
2019-2025
Nanjing Second Hospital
2012-2025
Binghamton University
2021-2024
Second Affiliated Hospital of Xi'an Jiaotong University
2023
Nanjing Medical University
2015-2023
Stomatology Hospital
2020
Guangzhou Medical University
2020
Southeast University
2012-2019
Nanjing Traditional Chinese Medicine Hospital
2019
Nanjing University
2019
Abstract Extracellular nanovesicles (ENV) released by many cells contain lipids, proteins, and nucleic acids that contribute to intercellular communication. ENVs have emerged as biomarkers therapeutic targets but they also been explored drug delivery vehicles. However, for the latter application, clinical translation has limited low yield inadequate targeting effects. ENV vectors with desired properties can be produced from parental engineered express membrane-bound ligands, or generated...
Abstract Cell-derived small extracellular vesicles have been exploited as potent drug vehicles. However, significant challenges hamper their clinical translation, including inefficient cytosolic delivery, poor target-specificity, low yield, and inconsistency in production. Here, we report a bioinspired material, engineered fusogen targeting moiety co-functionalized cell-derived nanovesicle (CNV) called eFT-CNV, vehicle. We show that universal eFT-CNVs can be produced by extrusion of...
Extracellular vesicles (EVs) are lipid-bilayer-enclosed of submicron size that secreted by various cells. As mediators intercellular communication, EVs can alter the physiological state recipient cells delivering encapsulated proteins and nucleic acids. Incontestably, growing evidence has shown important biological roles clinical relevance EVs. The use stem cell-derived as a cell-free therapeutic modality for skin treatment emerged promising application in dermatology. However, moderate...
Patients with non-small-cell lung cancer (NSCLC) frequently develop radioresistance, resulting in poor response to radiation and unfavourable prognosis. Early detection of radioresistance hence can guide the adjustment treatment regimens time. Exosomes are lipid bilayer-enclosed vesicles sub-micrometer size that released by various cells. contain a tissue-specific signature wherein variety proteins nucleic acids selectively packaged. Growing evidence shows exosomes involved pathophysiology...
Immune checkpoint inhibitors (ICI) targeting PD-1/PD-L1 have been approved for the treatment of a variety cancers. However, efficacy antibody-based ICIs could be further improved by mitigating anti-drug antibodies, proteolytic cleavage, and on-target off-tumor toxicity. One strategy accomplishing this is through use extracellular vesicles (EVs), cell derived submicron with many unique properties. We constructed an engineered MDA-MB-231 line harvesting EVs. This was accomplished...
It is imperative to explore biomarkers that are both precise and readily accessible in the comprehensive management of breast cancer. A multicenter cohort, including 512 cancer patients 198 nonneoplastic individuals, was recruited detect level tumor-derived extracellular vesicles using our method based on dual DNA tetrahedral nanostructures. The significantly higher newly diagnosed than individuals at a cutoff value 3.58 U/μL. For postoperative metastasis monitoring, with those without 3.91...
Circulating tumor cells (CTCs) have attracted significant interest as a biomarker for cancer diagnosis. In this study, we judiciously constructed recombinant MUC1-dependent adenovirus (rAdF35-MUC1) that can selectively replicate and overexpress copepod super green fluorescent proteins (copGFP) in MUC1-positive to investigate its role the detection of CTCs. We conducted comparative study between rAdF35-MUC1 existing hTERT-dependent (rAdF35-hTERT). Breast cell lines healthy human peripheral...
Extracellular vesicles (EVs) play a crucial role as important mediators of intercellular communication in the progression tumors. The capture and analysis tumor-derived EVs offer new possibilities for application cancer liquid biopsies. This study aims to construct DNA tetrahedral nanostructure that specifically recognizes HER2 CD63, enabling effective enrichment detection HER2-expressing (HEVs). We enriched HEVs from cell lines 13 random clinical samples validated their characteristics by...
Biocompatible gold nanoparticles (GNPs) are potentially practical and efficient agents in cancer radiotherapy applications. In this study, we demonstrated that GNPs can significantly modulate irradiation response of hepatocellular carcinoma cells vitro investigated the underlying mechanisms. We co-grafted galactose (GAL) targeting hepatocyte specific asialoglycoprotein receptor Polyethylene Glycol (PEG) onto surfaces to increase specificity stability.This novel GAL-PEG-GNPs bare show similar...
Endo-/lysosome escape is a major challenge in nanoparticle-based protein delivery for cancer therapy. To enhance the endo-/lysosomal and increase efficacy of delivery, current strategies mainly focus on destroying endo-/lysosomes by employing modified nanoparticles, such as pH-sensitive polyplexes, cell-penetrating peptides, photosensitive molecules. Herein, we hypothesize that pretreatment with empty nanocarriers might make occupied affect endo/lysosomal subsequently nanocarriers. We first...
Stem cells from human exfoliated deciduous teeth (SHEDs) are ideal seed in bone tissue engineering. As a first-line antidiabetic drug, metformin has recently been found to promote formation. The purpose of this study was investigate the effect on osteogenic differentiation SHEDs and its underlying mechanism. were isolated dental pulp healthy children aged 6 12, their surface antigen markers stem detected by flow cytometry. (10–200 μM) treatment cell viability, proliferation, analyzed....
The multifaceted interactions among the immune system, cancer cells and microbial components have established a novel concept of immuno-oncology-microbiome (IOM) axis. Microbiome sequencing technologies played pivotal role in not only analyzing how gut microbiota affect local distant tumors, but also providing unprecedented insights into intratumor host-microbe interactions. Herein, we discuss emerging trends transiting from bulk-level to single cell- spatial-level analyses. Moving forward...
Abstract Computed tomography-guided radiofrequency ablation (CT-RFA) and laparoscopic RFA (L-RFA) have been used to treat intrahepatic recurrent small hepatocellular carcinoma (HCC) against the diaphragmatic dome. However, therapeutic safety, efficacy, hospital fee never compared between two techniques due scarcity of cases. In this retrospective study, 116 patients were divided into groups with a total 151 local HCC lesions abutting diaphragm. We overall survival (OS), tumor progression...
In this study, a novel RNA aptamer biochip was developed for tumor cell capture and detection of single resistance. This consists polydimethylsiloxane (PDMS) cover containing channel introducing cells sustaining their activity microelectrode matrix on silicon dioxide layer. Epidermal growth factor receptor (EGFR) aptamers which specifically identify isolate were attached in the gap between two electrodes. After incubation, surplus removed, those dwelling intervals further analyzed. When...
Abstract Background Aberrant DNA methylation is an epigenetic marker that has been linked to the pathogenesis of colorectal cancer (CRC). Long noncoding RNAs (lncRNAs) have increasingly identified be associated with tumorigenic processes CRC. Identifying epigenetically dysregulated lncRNAs and characterizing their effects during carcinogenesis are focuses research. Methods Differentially methylated loci expressed were by integrating methylome transcriptome analyses using The Cancer Genome...
There have been no reports about the application of random survival forest (RSF) model to predict disease progression HIV-associated B-cell lymphoma.
Despite advances in targeted therapies, primary and acquired resistance make the treatment of colorectal cancer (CRC) a pressing issue to be resolved. According reports, development CRC is linked miRNA dysregulation. Multiple studies have demonstrated that miR-135b-5p has an aberrant expression level between tissues adjacent tissues. However, it unclear whether there correlation cetuximab (CTx) CRC. Use GEO database measure Additionally, RT-qPCR was applied ascertain production three human...
To explore whether metformin reduces cardiotoxicity of doxorubicin through the AMPK pathway.We analyzed data 123 patients with myeloid leukemia, non-Hodgkin's lymphoma, or breast cancer receiving for phased chemotherapy, including 43 combined treatment (test group) and 80 without (control group). The changes in plasma levels CK-MB, LDH, BNP, left ventricular ejection fraction (EF) fractional shortening (FS) were observed. effect treatments doxorubicin, alone combination, on myocardial...
Wntless also known as WLS, GPR 177, or Evi, is a key modulator of Wnt protein secretion. Its overexpression found in certain types human cancers such malignant astrocytoma and breast cancers. We hypothesized that this may be aberrantly expressed colorectal carcinoma which possesses aberrant signaling. To investigate the association between expression clinicopathological parameters carcinomas, set tissue samples was analyzed for WLS using an anti‐ 177 monoclonal antibody specific protein....
Immunotherapy, particularly the utilization of immune checkpoint inhibitors (ICIs), assumes a pivotal role in comprehensive management advanced lung cancer. There has been substantial deliberation regarding appropriateness extending ICIs treatment beyond point disease progression. This study delves into potential benefits sustained subsequent to progression patients.A retrospective analysis was conducted on cohort 248 patients diagnosed with cancer who received ICIs. The population comprised...
The high atomic number of gold nanoparticles (GNPs) enables them to offer potential as practical and efficient radiosensitizing agents for cancer radiotherapy applications. In the present study, it was demonstrated that GNPs can significantly modulate irradiation response hepatocellular carcinoma (HCC) cells in vitro vivo, which underlying mechanisms were investigated. Cetuximab (C225) is a targeting agent, binds extracellular domain epidermal growth factor receptor (EGFR)....
BACKGROUND According to current statistics, renal cancer accounts for 3% of all cancers worldwide. Renal cell carcinoma (RCC) is the most common solid lesion in kidney and approximately 90% malignancies. Increasing evidence has shown an association between immune infiltration RCC clinical outcomes. To discover possible targets system, we investigated link tumor-infiltrating cells (TIICs) prognosis RCC. AIM investigate effects 22 TIICs on patients identify potential therapeutic immunotherapy....