A5114929547- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- T-cell and B-cell Immunology
- Immunodeficiency and Autoimmune Disorders
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- Tissue Engineering and Regenerative Medicine
- Cancer-related gene regulation
- Bacteriophages and microbial interactions
- Cardiomyopathy and Myosin Studies
- Extracellular vesicles in disease
- Cardiovascular Effects of Exercise
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Nuclear Structure and Function
- Electrospun Nanofibers in Biomedical Applications
- Circular RNAs in diseases
- Adenosine and Purinergic Signaling
- Chronic Kidney Disease and Diabetes
- Vitamin C and Antioxidants Research
- Skin and Cellular Biology Research
- Hedgehog Signaling Pathway Studies
Heilongjiang Institute of Technology
2024-2025
Central South University
2013-2024
Second Xiangya Hospital of Central South University
2014-2024
Peking University People's Hospital
2023-2024
Peking University
2023-2024
Tongji University
2022-2024
Soochow University
2016-2023
First Affiliated Hospital of Soochow University
2016-2023
Lanzhou Veterinary Research Institute
2023
Chinese Academy of Agricultural Sciences
2023
Mesenchymal stem cells (MSCs) repair infarcted heart through paracrine mechanism. We sought to compare the effectiveness of MSCs and MSC-derived exosomes (MSC-Exo) in repairing hearts identify how MSC-Exo mediated cardiac is regulated. In a rat myocardial infarction model, we found that inhibited fibrosis, inflammation, improved function. The beneficial effects were significantly superior compared MSCs. To explore potential mechanisms underlying MSC-Exo’s effects, performed several vitro...
N6-methyladenosine (m6A) is an important epitranscriptomic mark with high abundance in the brain. Recently, it has been found to be involved regulation of memory formation and mammalian cortical neurogenesis. However, while now established that m6A methylation occurs a spatially restricted manner, its functions specific brain regions still await elucidation. We identify widespread dynamic RNA developing mouse cerebellum further uncover distinct features continuous temporal-specific across...
Exosomes derived from mesenchymal stem cells (MSCs) were proved to boost cell proliferation and angiogenic potency. We explored whether cardiac (CSCs) preconditioned with MSC exosomes could survive function better in a myocardial infarction model.DiI-labeled internalized CSCs. They stimulated proliferation, migration, angiotube formation of CSCs dose-dependent manner. In rat model, exosome-preconditioned had significantly survival, enhanced capillary density, reduced fibrosis, restored...
Abstract Argonaute (Ago) proteins are conserved nucleic acid-guided present in all domains of life. Eukaryotic (eAgos) key players RNA interference pathways and function as RNA-guided endonucleases at physiological temperatures. Although eAgos considered to evolve from prokaryotic (pAgos), previously studied pAgos were unable catalyze cleavage Here, we describe a distinctive pAgo mesophilic bacteria Kurthia massiliensis (KmAgo). KmAgo utilizes DNA guides cleave single-stranded (ssDNA)...
SHH subgroup medulloblastoma (SHH-MB) is one of the most common malignant pediatric tumors that arises in cerebellum. Previously, we showed RNA m6A methylation participates regulation cerebellar development. Here investigate whether dysregulated contributes to tumorigenesis SHH-MB. We show high expression methyltransferase METTL3 associates with worse survival patients A large number hypermethylated transcripts are identified SHH-MB tumor cells by m6A-seq. find promotes progression via...
Non-small cell lung cancer (NSCLC) is the most common type of cancer, and multiple evidence has confirmed that C5a production elevated in NSCLC microenvironment. Although proliferation induced by been reported, involved mechanism not elucidated. In this study, we examined proliferation-related genes (i.e., KLF5, GCN5, GDF15) receptor (C5aR) expression tumor tissues as well concentration plasma patients, then determined roles GDF15 C5a-triggered related both vitro vivo. Our results found...
The Arabidopsis FERONIA ( FER ) receptor kinase is a key hub of cell signaling networks mediating various hormone, stress, and immune responses. Previous studies have shown that functions correlate with auxin responses, but the underlying molecular mechanism unknown. Here, we demonstrate primary root fer‐4 mutant displays increased lateral branching delayed gravitropic response, which are associated polar transport PAT ). Our data suggest aberrant PIN 2 polarity responsible for response in ....
Argonaute (Ago) proteins are programmable nucleases found in eukaryotes and prokaryotes. Prokaryotic Agos (pAgos) share a high degree of structural homology with eukaryotic (eAgos), eAgos originate from pAgos. Although exclusively cleave RNA targets, most characterized pAgos DNA targets. This study novel pAgo, MbpAgo, the psychrotolerant bacterium Mucilaginibacter paludis which prefers to targets rather than Compared previously studied Agos, MbpAgo can utilize both 5'phosphorylated(5'P)...
Proper localization and anchorage of nuclei within skeletal muscle is critical for cellular function. Alterations in nuclear anchoring proteins modify a number functions including mechanotransduction, localization, chromatin positioning/compaction overall organ In muscle, nesprin 1 desmin are thought to link the nucleus cytoskeletal network. Thus, we hypothesize that both these factors play key role To examine this question, utilized global ablation murine models 1, or desmin. Herein, have...
Purpose: Most recently, circular RNAs (circRNAs) were considered playing regulatory roles in tumor initiation and development. The specific function of circRNAs hepatocellular carcinoma (HCC) remains unknown. This study was designed to detect a circRNA hsa_circ_0079299 HCC. Methods: expression HCC cell lines detected using quantitative PCR (qPCR). Cell proliferation, migration, cycle apoptosis after overexpression the measured counting kit-8 (CCK8) assay, colony formation,...
With the ongoing advancement in computer chip processing power, construction and optimization of biological models have become increasingly efficient highly feasible. This paper introduces a novel LSTM algorithm, which incorporates Attention mechanism, specifically designed to accelerate model building process. The adaptive mechanism employed by this algorithm allows it accurately capture most essential subtle features within complex systems. Additionally, dynamically adjusts parameters...
Arrhythmogenic cardiomyopathy (AC) is associated with mutations in genes encoding intercalated disc proteins and ultimately results sudden cardiac death. A subset of patients AC have the autosomal recessive cardiocutaneous disorder Naxos disease, which caused by a 2-base pair deletion plakoglobin-encoding gene JUP that truncated protein reduced expression. In mice, cardiomyocyte-specific plakoglobin deficiency recapitulates many aspects human AC, overexpression Naxos-associated also an...
Argonaute proteins are widespread in prokaryotes and eukaryotes with diversified catalytic activities. Here, we describe an from Marinitoga hydrogenitolerans (MhAgo) all eight cleavage Utilization of four types guides efficient single-stranded DNA (ssDNA) RNA targets revealed. The preference for the 5′-terminus nucleotides 5′P guides, but no obvious preferences that 5′OH is further uncovered. Moreover, efficiency heavily impaired by mismatches central 3′-supplementary regions affinity...
Acute myeloid leukemia (AML) is the most common hematopoietic malignancies, and chemotherapy resistance one of main causes relapse. Because lower survival rate for patients with relapse, it pivotal to identify etiological factors responsible chemo-resistance. In this work, direct MeRIP-seq analysis sequential samples at stage complete remission (CR) relapse identifies that dysregulated N6-methyladenosine (m6A) methylation involved in progression, hypomethylated RNAs are related cell...
Ovarian cancer is a type of gynecological with the highest mortality rate worldwide. Due to lack effective screening methods, most cases are diagnosed at later stages where survival rates poor. Thus, it termed 'silent killer' and lethal all malignancies in women. IQ motif containing GTPase Activating Protein 3 (IQGAP3) member Rho family GTPases, plays crucial role development progression several types cancer. The aim present study was investigate oncogenic functions mechanisms IQGAP3 on...
High mobility group box 1 (HMGB1), which is released by activated immune cells and necrotic cells, has properties similar to those of pro-inflammatory cytokines. Cardiopulmonary bypass (CPB) induces systemic inflammation aortic cross-clamping myocardial ischemia. This study was conducted observe the dynamic changes HMGB1 tumor necrosis factor (TNF)-α levels during CPB analyze their clinical significance. A total 78 cases American Society Anesthesiologists (ASA) grade II-IV undergoing...
Dysregulation of cardiac transcription programs has been identified in patients and families with heart failure, as well those morphological functional forms congenital defects. Mediator is a multi-subunit complex that plays central role initiation by integrating regulatory signals from gene-specific transcriptional activators to RNA polymerase II (Pol II). Recently, subunit 30 (MED30), metazoan specific subunit, associated Langer-Giedion syndrome (LGS) Type Cornelia de Lange syndrome-4...
Protein phosphatases are post-translational regulators of Toxoplasma gondii proliferation, tachyzoite-bradyzoite differentiation and pathogenesis. Here, we identify the putative protein phosphatase 6 (TgPP6) subunits T . elucidate their role in parasite lytic cycle. The catalytic subunit TgPP6C regulatory TgPP6R likely form a complex whereas predicted structural TgPP6S, with low homology to human PP6 subunit, does not coassemble TgPP6R. Functional studies showed that essential for growth...
Emery-Dreifuss muscular dystrophy, caused by mutations in genes such as emerin (EMD) or lamin A/C (LMNA), is a disorder affecting the joints, muscles, and heart, with wide spectrum of patient phenotypes including muscle wasting cardiac conduction defects.Here we report multi-generation family from Hunan Province China. Affected members displayed an uncommon clinical presentation serious abnormalities at early age high incidence sudden death along mild skeletal atrophy joint contracture....
Amyloid-β, a hallmark of Alzheimer’s disease, forms toxic intracellular oligomers and extracellular senile plaques resulting in neuronal toxicity. Ethanol is widely consumed worldwide. Moderate ethanol consumption has numerous benefits humans. We found that could significantly extend the lifespan Caenorhabiditis elegans previous study. Based on study, we tested effect disease transgenic strain CL4176, which expresses amyloid-β1-42 peptide body wall muscle cells. delayed paralysis reduced...
Lung cancer patients with lymph node metastasis usually had short overall survival and occurred distant metastases at the early stage. However, some of these people did have more prolonged survival. The underlying reason is still unclear. In this study, we found a novel molecule, family sequence similarity 136, member A gene (FAM136A). First, performed immunohistochemistry for FAM136A in 177 lung carcinoma tissues. Second, carried out vitro studies by using A549 PC-9. We detected...