A5100726490- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Ferroptosis and cancer prognosis
- Lung Cancer Treatments and Mutations
- PI3K/AKT/mTOR signaling in cancer
- Cancer, Lipids, and Metabolism
- Cancer Genomics and Diagnostics
- RNA and protein synthesis mechanisms
- Extracellular vesicles in disease
- Receptor Mechanisms and Signaling
- RNA Research and Splicing
- Cancer Mechanisms and Therapy
- Epigenetics and DNA Methylation
- Cancer Immunotherapy and Biomarkers
- Metabolism, Diabetes, and Cancer
- Cancer-related gene regulation
- Genetic Syndromes and Imprinting
- Endoplasmic Reticulum Stress and Disease
- Hepatocellular Carcinoma Treatment and Prognosis
- Inflammatory mediators and NSAID effects
- Peroxisome Proliferator-Activated Receptors
- interferon and immune responses
- Cholangiocarcinoma and Gallbladder Cancer Studies
Zhengzhou University
2021-2024
Henan Cancer Hospital
2021-2024
Kunming Medical University
2011-2024
Hunan Normal University
2019-2021
Yangtze University
2021
Hunan Provincial People's Hospital
2021
Chinese Academy of Medical Sciences & Peking Union Medical College
2021
Yunnan Institute of Parasitic Diseases
2020
Zhuzhou Central Hospital
2019
Central South University
2019
Incorporation of next-generation sequencing (NGS) technology into clinical utility in targeted and immunotherapies requires stringent validation, including the assessment tumor mutational burden (TMB) microsatellite instability (MSI) status by NGS as important biomarkers for response to immune checkpoint inhibitors.We designed an assay, Cancer Sequencing YS panel (CSYS), applied algorithms detect five classes genomic alterations two features TMB MSI.By CSYS exhibited high sensitivity...
Radiation-induced lung fibrosis (RILF) is a common complication of thoracic radiotherapy. Alveolar epithelial cells play crucial role in via epithelial-mesenchymal transition (EMT). Exosomes derived from mesenchymal stem own the beneficial properties to repair and regeneration damaged tissues, however underlying mechanisms remain poorly understood.Mouse cells-derived exosomes (mMSCs-Exo) were isolated by differential centrifugation, their protective effects assessed vivo vitro, respectively....
Air pollution has been classified as Group 1 carcinogenic to humans, but the underlying tumorigenesis remains unclear. In Xuanwei City of Yunnan Province, lung cancer incidence is among highest in China attributed severe air generated by combustion smoky coal, providing a unique opportunity dissect carcinogenesis pollution. Here we analyzed somatic mutations 164 non-small cell cancers (NSCLCs) from and control regions (CR) where coal was not used. Whole genome sequencing revealed mean 289...
Long noncoding RNAs (lncRNAs), a new class of functional regulators involved in human tumorigenesis, have been attracting the increasing attention researchers. The lncRNA colorectal neoplasia differentially expressed (CRNDE) gene, transcribed from chromosome 16 on strand opposite adjacent IRX5 was originally found to be increased CRC and reported abnormally many cancers. However, its potential role molecular mechanism underlying radioresistant phenotype formation lung adenocarcinoma (LAD)...
Nemo-like kinase (NLK) is an evolutionarily conserved serine/threonine that regulates the activity of a wide range signal transduction pathways. Metformin, oral antidiabetic drug, used for cancer prevention. However, significance and underlying mechanism NLK metformin in oncogenesis has not been fully elucidated. Here, we investigate novel role human non-small cell lung (NSCLC). expression was analyzed 121 NSCLCs 92 normal tissue samples from benign pulmonary disease. Lentivirus vectors with...
Background/Aims: This study aimed to investigate the potential roles of miR-424 expression in non-small cell lung cancer (NSCLC) metastasis and growth its underlying mechanism. Methods: The two NSCLC lines (A549 H1975) was altered by transfection with mimic inhibitor. Effects overexpression suppression on cells migration, invasion colony formation were analyzed. Target genes for predicted using bioinformatics method then verified luciferase assay. proliferation reanalyzed condition TNFAIP1...
// Jiang Chen 1, 2, * , Tong Ji Jie Zhao 2 Gaofeng Li Jian Zhang 1 Renan Jin Jinghua Liu Xiaolong Xiao Liang Diyu Huang Anyong Xie 3 Hui Lin Yong Cang Xiujun Cai Department of General Surgery, Sir Run Shaw Hospital, College Medicine, Zhejiang University, Hangzhou, Zhejiang, 310058, China Life Sciences Institute and Innovation Center for Cell Signalling Network, 310029, Translational These authors have contributed equally to this work Correspondence to: Cai, e-mail: cxjzu@hotmail.com Cang,...
Abstract Lung cancer is the leading cause of cancer-related deaths worldwide, and non-small-cell lung (NSCLC) accounts for about 80% all cases, which major subgroup cancer. G protein-coupled receptor kinase 5 (GRK5) has been demonstrated to play pivotal roles in both development progression several pathological conditions including Here, we found that GRK5 expression was significantly increased 539 NSCLC cancerous tissues than 99 normal non-cancerous by immunohistochemistry analysis; also...
Lung cancer is one of the common malignant tumors worldwide with high incidence and mortality. MicroRNA-423-3p (miR-423-3p) acts as an oncogene in several types cancers. The aim this study to reveal clinical significance biological function miR-423-3p lung cancer.The expression was detected specimens by reverse transcription-quantitative polymerase chain reaction (qRT-PCR) assay. Kaplan-Meier survival Cox regression analyses were used investigate prognostic cancer. CCK-8 Transwell assays...
Long noncoding RNA (lncRNA) extracellular leucine rich repeat and fibronectin type III domain containing 1-antisense 1 (ELFN1-AS1) has been found to be upregulated in various tumors. However, the biological functions of ELFN1-AS1 gastric cancer (GC) are not entirely understood. In present study, expression levels ELFN1-AS1, miR-211-3p, TRIM29 determined using reverse transcription-quantitative PCR. Subsequently, CCK8, EdU, colony formation assays performed determine GC cell vitality. The...
Air pollution significantly impacts lung cancer progression, but there is a lack of comprehensive molecular characterization clinical samples associated with air pollution. Here, we performed proteogenomic analysis adenocarcinoma (LUAD) in 169 female never-smokers from the Xuanwei area (XWLC cohort), where coal smoke primary contributor to high incidence. Genomic mutation revealed XWLC as distinct subtype LUAD separate cases smoking or endogenous factors. Mutational signature suggested that...
Lung cancer is the most common type worldwide, and investigating novel therapeutics methods for treatment of chemoresistant lung are notable clinical significance. Reverse transcription-quantitative polymerase chain reaction western blotting assays were performed to analyze expression levels phosphatase tensin homolog (PTEN) microRNA-4328 (miR-4328), Cell Counting Kit-8 (CCK-8) Transwell migration conducted evaluate proliferation A549 cells, respectively. Everolimus was observed upregulate...
Lung cancer is the leading cause of cancer-associated mortality worldwide. In China, in particular, lung has markedly increased and likely to continue rise. RNA-binding proteins are pivotal development progression a variety types, including non-small cell (NSCLC). motif protein 47 (RBM47) been found act as tumor suppressor breast NSCLC. However, best our knowledge, RBM47 expression NSCLC tissues yet be investigated. Analysis via online database, Gene Expression Omnibus, revealed that was...
Lung adenocarcinoma accounts for more than 50% of non-small cell lung cancers. Dysregulated microRNAs (miRNAs) and coding genes play a critical role in irradiation resistance might be promising therapeutic targets. In the present study, we demonstrate effect miR-4306/IGF2R axis on malignant behaviors cells response to irradiation.Quantitative realtime-PCR Western blot assays were applied miR-4306 IGF2R expression tumors cells. A CCK-8 assay kit was used detect viability. Colony formation...
Lung cancer patients with lymph node metastasis usually had short overall survival and occurred distant metastases at the early stage. However, some of these people did have more prolonged survival. The underlying reason is still unclear. In this study, we found a novel molecule, family sequence similarity 136, member A gene (FAM136A). First, performed immunohistochemistry for FAM136A in 177 lung carcinoma tissues. Second, carried out vitro studies by using A549 PC-9. We detected...
Abstract This study aims to analyze the RNA expression and alternative polyadenylation (APA) events identify APA tuned genes with prognostic significance in lung adenocarcinoma (LUAD). Genome‐wide profile were acquired LUAD cancer normal samples GSE197346. Comparative analysis screened common deregulated transcripts. All 11 19 transcripts up down expressed polyadenylated samples, respectively. Clinical found eight significance, such as coiled‐coil domain containing 137 (CCDC137). Role of...
Background The biological function of distal-less homeobox 1 (DLX1) in lung adenocarcinoma (LUAD) remains unclear, despite a growing body evidence that DLX1 is involved the initiation and progression various tumors. Methods This study explored confirmed prognostic immunologic roles LUAD via bioinformatic analysis cellular functional validation. MethSurv was used to analyze DNA methylation levels value CpG islands. mutation rates prognoses between patients with without mutated gene were...
Air pollution significantly impacts lung cancer progression, but there is a lack of comprehensive molecular characterization clinical samples associated with air pollution. Here, we performed proteogenomic analysis adenocarcinoma (LUAD) in 169 female never-smokers from the Xuanwei area (XWLC cohort), where coal smoke primary contributor to high incidence. Genomic mutation revealed XWLC as distinct subtype LUAD separate cases smoking or endogenous factors. Mutational signature suggested that...
Lung cancer is a disease with high incidence rate in Yunnan province, yet there paucity of large-scale studies on its clinical epidemiology. This research aims to investigate the epidemiological characteristics patients who underwent lung surgery at Cancer Hospital over past decade, thereby providing theoretical basis for prevention and treatment cancer. Clinical data were collected from 15,967 between 2013 2022. A statistical analysis was conducted patients' general data, surgical...