A5016606284- Cell Image Analysis Techniques
- Advanced X-ray Imaging Techniques
- Single-cell and spatial transcriptomics
- Advanced X-ray and CT Imaging
- Bat Biology and Ecology Studies
- Zebrafish Biomedical Research Applications
- CRISPR and Genetic Engineering
- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- melanin and skin pigmentation
- Advanced Fluorescence Microscopy Techniques
- AI in cancer detection
- Digital Imaging for Blood Diseases
- Pharmaceutical industry and healthcare
- Biomedical Ethics and Regulation
- Mycobacterium research and diagnosis
- Radiation Therapy and Dosimetry
- Digital Holography and Microscopy
- Microbial Fuel Cells and Bioremediation
- Animal testing and alternatives
- RNA and protein synthesis mechanisms
- Molecular Biology Techniques and Applications
Penn State Milton S. Hershey Medical Center
2018-2022
Pennsylvania State University
2016-2021
Yale University
2015
Organismal phenotypes frequently involve multiple organ systems. Histology is a powerful way to detect cellular and tissue phenotypes, but largely descriptive subjective. To determine how synchrotron-based X-ray micro-tomography (micro-CT) can yield 3-dimensional whole-organism images suitable for quantitative histological phenotyping, we scanned whole zebrafish, small vertebrate model with diverse tissues, at ~1 micron voxel resolutions. Micro-CT optimized characterization (histotomography)...
Ideal three-dimensional imaging of complex samples made up micron-scale structures extending over mm to cm, such as biological tissues, requires both wide field view and high resolution. For existing optics detectors used for micro-CT (computed tomography) imaging, sub-micron pixel resolution can only be achieved fields <2 mm. This article presents a unique detector system with 6 field-of-view image circle 0.5 µm size that in units utilizing synchrotron commercial X-ray sources. A...
For over a hundred years, the histological study of tissues has been gold standard for medical diagnosis because histology allows all cell types in every tissue to be identified and characterized. Our laboratory is actively working make technological advances X-ray micro-computed tomography (micro-CT) that will bring diagnostic power full volumes at cellular resolution (i.e., an Histo-tomography modality). Toward this end, we have made targeted improvements sample preparation pipeline. One...
We previously described X-ray histotomography, a high-resolution, non-destructive form of microtomography (micro-CT) imaging customized for three-dimensional (3D), digital histology, allowing quantitative, volumetric tissue and organismal phenotyping (Ding et al., 2019). Here, we have combined micro-CT with novel application ionic silver staining to characterize melanin distribution in whole zebrafish larvae. The resulting images enabled whole-body, computational analyses regional content...
Abstract Histological studies providing cellular insights into tissue architecture have been central to biological discovery and remain clinically invaluable today. Extending histology three dimensions would be transformational for research diagnostics. However, three-dimensional is impractical using current techniques. We customized sample preparation, synchrotron X-ray tomographic parameters, image analysis allow complete histological phenotyping whole larval juvenile zebrafish. The...
For over a hundred years, the histological study of tissues has been gold standard for medical diagnosis because histology allows all cell types in every tissue to be identified and characterized. Our laboratory is actively working make technological advances X-ray micro-computed tomography (micro-CT) that will bring diagnostic power full volumes at cellular resolution (i.e., an Histo-tomography modality). Toward this end, we have made targeted improvements sample preparation pipeline. One...
Abstract Melanin-rich zebrafish melanophores are used to study pigment development, human skin color, and as a large-scale screening phenotype. To facilitate more detailed whole-body, computational analyses of melanin content morphology, we have combined X-ray microtomography (micro-CT), non-destructive, full-volume imaging modality, with novel application ionic silver staining characterize distribution in whole larvae. Normalized micro-CT reconstructions silver-stained fish consistently...
Abstract Ideal 3-dimensional imaging of many complex samples, such as biological tissues made up micro-scale structures extending over millimeter- to centimeter-scale tissue samples and organisms, requires both a wide field-of-view high resolution. With existing optics detectors used for micro-CT imaging, sub-micron pixel resolution can only be achieved fields-of-view <2 mm. This manuscript presents unique detector system with 6-mm image circle 0.5 μm size that in synchrotron facilities...