Purple pericarps of bread wheat (Triticum aestivum L.) are a useful source dietary anthocyanins. Previous mapping results indicated that the purple pericarp trait is controlled by two complementary genes located on chromosomes 7D and 2A. However, identity mechanisms which they regulate unknown. In this study, transcription factors were characterised as anthocyanin activators in pericarps: TaPpm1 (purple pericarp-MYB 1) TaPpb1 pericarp-bHLH 1). Three non-functional variants detected coding...

Functional DNAs are valuable molecular tools in chemical biology and analytical chemistry but suffer from low activities due to their limited functionalities. Here, we present a chemoenzymatic method for site-specific installation of diverse functional groups on DNA, showcase the application this enhance catalytic activity DNA catalyst. Through introduction distinct groups, such as hydroxyl, carboxyl, benzyl, at specific positions, achieve significant enhancements RNA-cleaving deoxyribozyme...

The glossy varieties (A14 and Jing 2001) glaucous (Fanmai 5 Shanken 99) of wheat (Triticum aestivum L.) were selected for evaluation developmental changes in the composition morphology cuticular waxes on leaves spikes. results provide us with two different wax development patterns between leaf spike. general accumulation trend total load spike surfaces is first to increase then decrease during growth period, but these caused by compound classes Developmental mainly result variations alcohols...

Objectives Adiponectin is closely related to glucose metabolism and traditional diabetes risk factors (obesity, hypertension dyslipidaemia). We aimed explore the association between adiponectin levels newly diagnosed type 2 mellitus (T2DM) pre-diabetes in subgroups classified according T2DM factors. Setting Sun Yat-sen Memorial Hospital of University. Participants 3680 individuals (1753 men 1927 women) aged 18–70 years from Guangzhou Dongguan, China, were enrolled December 2018 October 2019....

Abstract To protect above‐ground plant organs from excessive water loss, their surfaces are coated by waxes. The genes involved in wax formation have been investigated detail Arabidopsis but scarcely crop species. Here, we aimed to isolate and characterize a CER1 enzyme responsible for of the very long‐chain alkanes present high concentrations especially during late stages wheat development. On basis comparative transcriptome analyses various organs, selected TaCER1‐1A as primary candidate...

Abnormal interactions between skin cells play an important role in the dysregulation of diabetic wound recovery. Exosomes are cell-derived lipid nanoparticles that transport messages cells, and isolating identifying potential therapeutic noncoding RNAs from exosomes is very important. We demonstrated treatment with Exos high glucose-pretreated immortalized human epidermal (HaCaT) (HG-Exos) could delay healing process mice. Further analysis indicated Exo-mediated uptake LINC01435 recipient...

Zinc sulfide (ZnS) is extensively utilized in various applications due to its exceptional optical transmittance across numerous spectral bands. To achieve ultra-high surface quality ZnS components, shear thickening polishing (STP) employed reduce roughness. A comparison between traditional fixed abrasive (FAP) and STP for glass showed that FAP results poor low removal efficiency uneven exposure, while provides better flexible process, proving feasibility advancement. The Taguchi method was...

Diabetic foot ulcers are recalcitrant to healing, and poor angiogenesis is considered as the main contributing factor. We aimed explore effect of extracellular vesicles (EVs) derived from wound fluids on new vessel formation in diabetic ulcers. EVs were isolated (DF-EVs). The inhibitory DF-EVs human umbilical vein endothelial cells (HUVECs) healing was tested. To elucidate potential mechanism these effects, we screened differentially expressed microRNAs (miRNAs) via microarray analysis...

This study had three objectives: (1) to investigate healthcare professionals' knowledge, attitude, and practice toward clinically applying graduated compression stockings, (2) analyze the influencing factors, (3) provide data support departments that develop health policies. A self-administered web-based survey was completed by 1,444 professionals, including physicians nurses from 15tertiary hospitals four secondary 10 provinces in China. Reliability analysis exploratory factor were used...

Cuticular wax accumulation and composition affects drought resistance in plants. Brachypodium distachyon plants subjected to water deficit polyethylene glycol (PEG) treatments resulted a significant increase total load, which very-long-chain (VLC) alkanes were more sensitive these than other compounds, implying that VLC biosynthesis plays important role distachyon. ECERIFERUM1 (CER1) has been reported encode core enzyme involved Arabidopsis (Arabidopsis thaliana), but few corresponding genes...

Accurate communication between fibroblasts and keratinocytes is crucial for diabetic wound healing. Extracellular vesicles are being explored as essential mediators of intercellular in the skin. However, mechanisms underlying healing mediated by fibroblast-derived extracellular (Fib-EVs) remain unclear. The present study evaluated role long noncoding RNA upregulated skin (lnc-URIDS) packed Fib-EVs streptozotocin-induced diabetes potential effects. We demonstrated that high glucose induced...

This study investigated effects of extruded diet (ED) and pelleted (PD) with differing dietary protein levels on growth performance nutrient utilization in juvenile largemouth bass (LMB). Nine hundred LMB (initial body weight 6.47 ± 0.1 g)were fed for eight weeks five crude (40%, 44%, 48%, 52% 56%) pelleting or extruding processing technology. The results showed the gain (WG, 380.65%), apparent digestibility (ADCP, 55.76%), dry matter (DM, 89.70%) whole-body (66.32%) ED52 group were...

Objective.Motor imagery (MI), based on the theory of mirror neurons and neuroplasticity, can promote motor cortical activation in neurorehabilitation. The strategy MI brain-computer interface (BCI) has been used rehabilitation training daily assistance for patients with hemiplegia recent years. However, it is difficult to maintain consistency timeliness receiving external stimulation neural most subjects owing high variability electroencephalogram (EEG) representation across trials/subjects....

Objective: This study aimed to explore the association between risk of newly diagnosed type 2 diabetes and galectin-3 adiponectin investigate whether their joint action shows a favorable assessment performance. Methods: We conducted community-based in 135 patients with 270 age- sex-matched nondiabetic patients. Odds ratios 95% confidence intervals were determined using logistic regression analysis. Receiver operating characteristic curve, decision curve analysis calibration plot used...

<title>Abstract</title> We report a case of early-onset hereditary thrombotic thrombocytopenic purpura in 16-years old girl, who suffered from solely thrombocytopenia and was misdiagnosed as idiopathic for years until two failed gestations finally revealed the underlying cause. Novel compound heterozygous mutations c.2865G&gt;A:p.Trp955X c.721delG:p.Gly241fs ADAMTS13 gene were found predicted to be associated with disease. She responded plasma therapy but renal dysfunction may remain longstanding.

With the development of brain-computer interface industry, large amounts related applications have entered people's vision. BCI based on steady-state visual evoked potentials (SSVEP) are widely used because they do not require pre-training and high information transmission rates. However, in actual use SSVEP stimulus paradigm, subjects will produce fatigue with use, affect efficiency. In this experiment, an experimental environment consisting two paradigm frequencies (7.5 Hz, 15 Hz), three...

We report a case of early-onset hereditary thrombotic thrombocytopenic purpura in 16-year-old girl who suffered from thrombocytopenia and was misdiagnosed with immune for years until two failed gestations finally revealed the underlying cause. The novel compound heterozygous mutation c.2865G > A:p.Trp955X c.721delG: p.Gly241fs ADAMTS13 gene were identified are predicted to be associated this disease. patient responded plasma therapy, including infusion exchange, but renal dysfunction may...

Context Some studies have investigated the application of sunflower meal (SFM) in feeding livestock and poultry. However, there been few researches related to fermenting SFM. Aims In this experiment, neutral detergent fibre degradability increase rate crude protein were used as rapid evaluation indicators solid-state fermentation (SSF), response-surface methodology was optimise process conditions for SSF SFM with mixed agent (Bacillus subtilis H-12, Saccharomyces cerevisiae Y-5 Trichoderma...

Compared with the light-flashing paradigm, ring-shaped motion checkerboard patterns avoid uncomfortable flicker or brightness modulation, improving practical interactivity of brain-computer interface (BCI) applications. However, due to fewer harmonic responses and more concentrated frequency energy elicited by patterns, mainstream untrained algorithms such as canonical correlation analysis (CCA) filter bank (FBCCA) methods have poor recognition performance low information transmission rate...

目的:探讨黄连素对链脲佐菌素所致糖尿病大鼠胰岛素抵抗的治疗作用。方法:采用链脲佐菌素腹腔注射的方式建立大鼠糖尿病模型,将实验大鼠分为5组:分别为模型组、黄连素低(100 mg/kg)、黄连素中(200 mg/kg)、黄连素高(300 mg/kg)剂量组和阳性对照组(二甲双胍:50 mg/kg),每组10只。另取10只正常大鼠作为正常组。黄连素低、中、高剂量组小鼠每天分别灌胃100、200和300 mg/kg黄连素;对照组每天灌胃二甲双胍50...