A5100337415- Advanced biosensing and bioanalysis techniques
- Circular RNAs in diseases
- DNA and Nucleic Acid Chemistry
- Endometriosis Research and Treatment
- RNA Research and Splicing
- Immune cells in cancer
- Biosensors and Analytical Detection
- RNA modifications and cancer
- Hydraulic Fracturing and Reservoir Analysis
- Bacteriophages and microbial interactions
- Reproductive System and Pregnancy
- Muscle Physiology and Disorders
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Genetic diversity and population structure
- Nanoplatforms for cancer theranostics
- Extracellular vesicles in disease
- Single-cell and spatial transcriptomics
- Pomegranate: compositions and health benefits
- RNA Interference and Gene Delivery
- Ferroptosis and cancer prognosis
- Cancer Genomics and Diagnostics
- Drilling and Well Engineering
- Cancer Cells and Metastasis
- Metabolomics and Mass Spectrometry Studies
University of Hong Kong
2022-2025
Hunan Agricultural University
2022-2024
Heilongjiang Bayi Agricultural University
2024
Southeast University
2024
State Key Laboratory of Digital Medical Engineering
2024
Shandong University
2022-2024
China University of Petroleum, Beijing
2023-2024
Guizhou University of Finance and Economics
2024
Nanjing University
2019-2023
China Agricultural University
2023
Abstract Molecular mechanisms underlying breast cancer lymph node metastasis remain unclear. Using single-cell sequencing, we investigated the transcriptome profile of 96,796 single cells from 15 paired samples primary tumors and axillary nodes. We identified nine cell subclusters including CD44 + / ALDH2 /ALDH6A1 stem (BCSCs), which had a copy-number variants similar to that normal tissue. Importantly, BCSCs existed only in evolved into metastatic clusters infiltrating Furthermore, data...
Triple-negative breast cancer (TNBC) is highly aggressive with a poor clinical prognosis and no targeted therapy. The c-Myc protein master transcription factor potential therapeutic target for TNBC. In this study, we develop PROTAC (PROteolysis TArgeting Chimera) based on TNA (threose nucleic acid) DNA that effectively targets degrades c-Myc. aptamer selected in vitro to bind the c-Myc/Max heterodimer appended E-box sequence create high-affinity, biologically stable bivalent binder. TNA-E...
<italic>In vitro</italic> selected threose nucleic acid (TNA) aptamers blocked PD-1/PD-L1 interaction and significantly inhibited tumour growth in xenograft mouse model.
Threose nucleic acid (TNA) has been considered a potential RNA progenitor in evolution due to its chemical simplicity and base pairing property. Catalytic TNA sequences with ligase activities might have facilitated the transition world. Here we report isolation of enzymes by vitro selection. The identified enzyme T8-6 catalyzes formation 2′–5′ phosphoester bond between 2′,3′-diol 5′-triphosphate group, kobs 1.1 × 10–2 min–1 (40 mM Mg2+, pH 9.0). For efficient reaction, requires UA|GA at...
Extracellular vesicles (EVs) are cell-derived, membrane-bound that carry molecular cargo to facilitate communication between cells. During pregnancy, EVs secreted by the syncytiotrophoblast layer of placenta villi, where they mediate functions resident leukocytes and invading extravillous trophoblasts (EVTs) in decidua. This study aims isolate placental (pEVs) from explant examine their regulatory roles on EVT endothelial cell functions. pEVs were successfully isolated ex vivo cultured...
The first demonstration of direct sequencing 2′-deoxy-2′-fluoroarabinonucleic acid (FANA) using Nanopore-Induced Phase-Shift Sequencing (NIPSS).
Exosomes are nanoscale membrane vesicles actively released by cells and play an important role in the diagnosis of cancer-related diseases. However, it is challenging to efficiently enrich exosomes from extracellular fluids. In this work, we used DNA nanostructures as "nanoweights" during centrifugation facilitate enrichment cancerous human serum. Two different tetrahedral (DTNs), each carrying a specific aptamer for exosome biomarker recognition, were incubated with clinical samples...
Introduction: The development of skeletal muscle is regulated by regulatory factors genes and non-coding RNAs (ncRNAs). Methods: objective this study was to understand the transformation fiber type in longissimus dorsi male Ningxiang pigs at four different growth stages (30, 90, 150, 210 days after birth, n = 3) histological analysis whole transcriptome sequencing. Additionally, investigated expression patterns various involved constructed a network for competing endogenous RNA (ceRNA) that...
The processes of muscle growth and development, including myoblast proliferation, migration, differentiation, fusion, are modified by a variety regulatory factors. MYL4 plays an important role in atrial cardiomyopathy, muscle-fiber size, development. structural variation (SV) was found via the de novo sequencing Ningxiang pigs, existence SV verified experiments. genotype distribution pigs Large White detected, it that were mainly BB AB genotype. However, molecular mechanisms behind...
MicroRNAs (miRNAs) are endogenous small non-coding RNAs that play crucial regulatory roles in many biological processes, including the growth and development of skeletal muscle. miRNA-100-5p is often associated with tumor cell proliferation migration. This study aimed to uncover mechanism myogenesis. In our study, we found expression level was significantly higher muscle tissue than other tissues pigs. Functionally, this shows miR-100-5p overexpression promotes inhibits differentiation C2C12...
Muscle cell growth plays an important role in skeletal muscle development. Circular RNAs (circRNAs) have been proven to be involved the regulation of and In this study, we explored effect circTTN on myoblast its possible molecular mechanism. Using C2C12 cells as a functional model, authenticity was confirmed by RNase R digestion Sanger sequencing. Previous studies showed that overexpression inhibits proliferation differentiation. Mechanistically, recruits PURB protein Titin (TTN) promoter...
Aleutian disease (AD) is a common immunosuppressive in mink farms world-wide. Since the 1980s, counterimmunoelectrophoresis (CIEP) has been main detection method for infection with Mink Disease Virus (AMDV). In this study, six peptides derived from AMDV structural protein VP2 were designed, synthesized, and used as ELISA antigens to detect anti-AMDV antibodies sera of infected minks. Serum samples collected 764 minks five different provinces, analyzed by both CIEP (a gold standard) peptide...
Cancer immunotherapy has emerged as a promising strategy against triple-negative breast cancer (TNBC). One of the immunosuppressive pathways involves programmed cell death-1 (PD-1) and death ligand-1 (PD-L1), but many patients derived little benefit from PD-1/PD-L1 checkpoint blockades treatment. Prior research shown that MYC, master transcription amplifier highly expressed in TNBC cells, can regulate tumor immune microenvironment constrain efficacy immunotherapy. This study aims to...
Abstract Aim Pleistocene climate and associated environmental changes have influenced phylogeographic patterns of many species. These not only depend on a species’ life history but also vary regionally. Consequently, populations widespread species that occur in several biomes might display different evolutionary trajectories. We aimed to identify regional drivers diversification the common pheasant, widely distributed ecological generalist. Location Asia. Taxon Common pheasant Phasianus...
DNA nanotechnology is powerful in constructing programmable nanostructures with distinct dimensions, sizes, and shapes. However, natural molecules are prone to nuclease degradation, thus limiting the vivo applications of such nanostructures. 2′-Fluoroarabinonucleic acid (FANA) a chemically modified oligonucleotide similar base pairing properties exhibits superior physical chemical stabilities. In this work, FANA were used construct double crossover nanostructures, it was demonstrated that...