A5087659149- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Animal Genetics and Reproduction
- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- MicroRNA in disease regulation
- DNA Repair Mechanisms
- Eicosanoids and Hypertension Pharmacology
- Cardiomyopathy and Myosin Studies
- Cancer Research and Treatments
- Animal Nutrition and Physiology
- Cancer-related gene regulation
- FOXO transcription factor regulation
- Genomics and Phylogenetic Studies
- Genetic and phenotypic traits in livestock
- Prion Diseases and Protein Misfolding
- RNA Research and Splicing
- Lipid metabolism and biosynthesis
- Congenital heart defects research
- Cancer, Hypoxia, and Metabolism
- Wnt/β-catenin signaling in development and cancer
- Sperm and Testicular Function
- Neurological diseases and metabolism
- RNA modifications and cancer
- BRCA gene mutations in cancer
Hunan Agricultural University
2021-2024
University of Victoria
1999
The growth and development of the Longissimus Dorsi muscle are complex, playing an important role in determination pork quality. study at mRNA level is particularly crucial for finding molecular approaches to improving meat quality pig breeding. current utilized transcriptome technology explore regulatory mechanisms intramuscular fat (IMF) deposition three core developmental stages (natal stage on day 1, growing 60, finishing 210) Ningxiang pigs. Our results revealed 441 differentially...
Introduction: The development of skeletal muscle is regulated by regulatory factors genes and non-coding RNAs (ncRNAs). Methods: objective this study was to understand the transformation fiber type in longissimus dorsi male Ningxiang pigs at four different growth stages (30, 90, 150, 210 days after birth, n = 3) histological analysis whole transcriptome sequencing. Additionally, investigated expression patterns various involved constructed a network for competing endogenous RNA (ceRNA) that...
MicroRNAs (miRNAs) are endogenous small non-coding RNAs that play crucial regulatory roles in many biological processes, including the growth and development of skeletal muscle. miRNA-100-5p is often associated with tumor cell proliferation migration. This study aimed to uncover mechanism myogenesis. In our study, we found expression level was significantly higher muscle tissue than other tissues pigs. Functionally, this shows miR-100-5p overexpression promotes inhibits differentiation C2C12...
The processes of muscle growth and development, including myoblast proliferation, migration, differentiation, fusion, are modified by a variety regulatory factors. MYL4 plays an important role in atrial cardiomyopathy, muscle-fiber size, development. structural variation (SV) was found via the de novo sequencing Ningxiang pigs, existence SV verified experiments. genotype distribution pigs Large White detected, it that were mainly BB AB genotype. However, molecular mechanisms behind...
Advances in long-read sequencing technology and genome assembly provide an opportunity to improve the pig reveal full range of structural variations (SVs) between local Chinese European pigs. To date, little is known about genomes some unique indigenous breeds, such as Ningxiang pig. Here, we report a highly contiguous (NX) via integration PacBio single-molecule real-time sequencing, Illumina next-generation BioNano optical mapping Hi-C (chromosome conformation capture) approaches. The...
The aim is to optimize the dimethylacetamide (DMA) straw freezing technology of Black silkies rooster semen through handy patent equipment, screening formula basic extender and optimizing DMA addition method, then by comparing fertility frozen with pellet semen. After optimized, it extended Youxian Partridge drake result showed that sperm motility Lake Ravie (LR) group 64%, 49.57% hatchability 91.52%, all which are superior those FEB, Beltsville Poultry Semen Extender (BPSE) (P < 0.05)....
Epidemiological evidence points to an increased risk of breast cancer in ataxia telangiectasia (AT) heterozygote women. Previous attempts screen early onset or familial patients failed confirm association. The issue AT and late sporadic remained unresolved. We screened 47 women who developed later onset, for mutated (ATM) mutations. No mutations were found.
Muscle cell growth plays an important role in skeletal muscle development. Circular RNAs (circRNAs) have been proven to be involved the regulation of and In this study, we explored effect circTTN on myoblast its possible molecular mechanism. Using C2C12 cells as a functional model, authenticity was confirmed by RNase R digestion Sanger sequencing. Previous studies showed that overexpression inhibits proliferation differentiation. Mechanistically, recruits PURB protein Titin (TTN) promoter...
MicroRNAs (miRNAs) are key regulators involved in the myogenic process skeletal muscles. miR-708-5p plays an important role various biochemical and physiological processes, but its function myogenesis remain unclear. In this study, we first explored effects of on C2C12 proliferation differentiation by overexpression interference experiments. Then, predicted target genes analyzed their function. We found that was gradually increased during myoblast differentiation. Overexpression...
With the continuous improvement in living standards, people's demand for high-quality meat is increasing. Ningxiang pig has delicious of high nutritional value, and loved by consumers. However, its slow growth low yield seriously restrict efficient utilization. Gene expression internal driving force life activities, so order to fundamentally improve rate, it key explore molecular mechanism skeletal muscle development pigs. In this paper, boars were selected four stages (30 days: weaning...
Objective: To investigate the effect of treatment with different concentrations ammonium chloride (NH4Cl) on mRNA and protein expression hypoxia-inducible factor-1α (HIF-1α) vascular endothelial growth factor (VEGF) in Chang liver cells. Methods: Normal cells were cultured treated continuously NH4Cl (1.25, 2.5, 5 mmol/L). The normally used as controls. RNA extracted at 5th, 10th, 15th generations. Quantitative real-time PCR, immunohistochemistry, Western blot to measure HIF-1α VEGF. Results:...
Abstract Background Ningxiang pig is resistant to roughage, delicious in meat quality and rich polyunsaturated fatty acids. However, its growth rate slow yield low, so it urgent analyze the molecular mechanism of muscle development. Genes are important molecules involved In this study, transcripts difference tables at two developmental stages (30 days 150 after birth) were identified by transcriptome sequencing, their regulation development was further explored. Result A total 22,411 9,021...
The wnt signaling transduction pathway is divided into two types, that are un-canonical kind and canonical kind, Wnt10b one of the member type.This plays an important role in formation fat deposition.In order to investigate function gene pig, we acquired full-length cDNA porcine from longissimus dorsi muscle by Rapid Amplification End (RACE) method.The expression level six tissues was also explored through qPCR.Moreover, single nucleotide polymorphism (SNP) site identified PCR-RFLP, then its...