A5056399429- Pneumocystis jirovecii pneumonia detection and treatment
- Parasitic Infections and Diagnostics
- Biochemical and Molecular Research
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- HIV/AIDS drug development and treatment
- Amoebic Infections and Treatments
- Hormonal Regulation and Hypertension
- Adipokines, Inflammation, and Metabolic Diseases
- Blood properties and coagulation
- Viral Infections and Vectors
- Medical Imaging and Analysis
- RNA modifications and cancer
- Vitamin D Research Studies
- Spinal Fractures and Fixation Techniques
- Renal Transplantation Outcomes and Treatments
- 14-3-3 protein interactions
- Spine and Intervertebral Disc Pathology
- Steroid Chemistry and Biochemistry
- Pharmacogenetics and Drug Metabolism
- Mycobacterium research and diagnosis
- Origins and Evolution of Life
Nanjing University
2013-2022
Nanjing Traditional Chinese Medicine Hospital
2022
Soochow University
2021
Second Affiliated Hospital of Soochow University
2021
Collaborative Innovation Center of Advanced Microstructures
2021
Brandeis University
2010-2011
Threose nucleic acid (TNA) has been considered a potential RNA progenitor in evolution due to its chemical simplicity and base pairing property. Catalytic TNA sequences with ligase activities might have facilitated the transition world. Here we report isolation of enzymes by vitro selection. The identified enzyme T8-6 catalyzes formation 2′–5′ phosphoester bond between 2′,3′-diol 5′-triphosphate group, kobs 1.1 × 10–2 min–1 (40 mM Mg2+, pH 9.0). For efficient reaction, requires UA|GA at...
Cryptosporidium spp. cause acute gastrointestinal disease that can be fatal for immunocompromised individuals. These protozoan parasites are resistant to conventional antiparasitic chemotherapies and the currently available drugs treat these infections largely ineffective. Genomic studies suggest that, unlike other parasites, is incapable of de novo pyrimidine biosynthesis. Curiously, possess redundant pathways produce dTMP, one involving thymidine kinase (TK) second via thymidylate...
MPA (mycophenolic acid) is an immunosuppressive drug produced by several fungi in Penicillium subgenus Penicillium. This toxic metabolite inhibitor of IMPDH (IMP dehydrogenase). The MPA-biosynthetic cluster brevicompactum contains a gene encoding B-type IMPDH, IMPDH-B, which confers resistance. Surprisingly, all members the contain genes IMPDHs both A and B types, regardless their ability to produce MPA. Duplication occurred before independently acquisition MPAbiosynthetic cluster. Both P....
The filamentous fungus Penicillium brevicompactum produces the immunosuppressive drug mycophenolic acid (MPA), which is a potent inhibitor of eukaryotic IMP dehydrogenases (IMPDHs). IMPDH catalyzes conversion to XMP via covalent enzyme intermediate, E-XMP*; MPA inhibits by trapping E-XMP*. P. (Pb) contains two MPA-resistant IMPDHs, PbIMPDH-A and PbIMPDH-B, are 17- 103-fold more resistant than typically observed. Surprisingly, active sites these enzymes essentially identical those...
ObjectivePseudomonas putida SJTE-1 can degrade17β-estradiol (E2) efficiently, but its degradation mechanism is still unclear. Here we characterized a 17β-hydroxysteroid dehydrogenase 2 (17β-HSD2) and one AraC regulator responsible for oxidization regulation of E2 biodegradation. MethodsWe detected the transcription 17β-hsd2 araC by reverse quantitative PCR. We overexpressed 17β-HSD2 in Escherichia coli BL21(DE3) strain purified them with metal-ion affinity chromatography. enzymatic...
<b>Objective:</b> Synovial fibroblasts (RASF) in rheumatoid arthritis are important mediators of synovial inflammation and joint destruction. This study provides valuable suggestions for the prevention treatment RA by observing catalase intervention RASF exploring its possible mechanisms. <br/><b>Methods:</b> An vitro model interleukin (IL)-1β-induced inflammatory was established, cells were intervened with different concentrations catalpol (0, 20, 40, 60, 80, 100, 120, 140 μmol/L) 6 h. Cell...
Objective:To compare and analyze the safety effectiveness of oblique lateral interbody fusion (OLIF) technique conventional lumbar posterior laminar decompression for spinal stenosis (DLCS).
Abstract Elevated fibrinogen increases risk for cardiovascular and cerebrovascular diseases. The biological effects of the fibrin-derived peptide Bβ15–42 are different from fibrin promote vascular anti-inflammatory effects. Fragments cleavage by earthworm fibrinolytic enzyme A (EFEa) structurally similar to Bβ15–42. Therefore, we evaluated if EFEa have anti-atherosclerotic To investigate effect EFEa, used New Zealand rabbits fed a high cholesterol diet atherosclerosis. Human umbilical...