A5076740257- Steroid Chemistry and Biochemistry
- Microbial bioremediation and biosurfactants
- Pharmaceutical and Antibiotic Environmental Impacts
- Genomics and Phylogenetic Studies
- Pharmacogenetics and Drug Metabolism
- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Microbial Community Ecology and Physiology
- Chromium effects and bioremediation
- Polyamine Metabolism and Applications
- Enzyme Catalysis and Immobilization
- Enzyme Structure and Function
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Microbial Natural Products and Biosynthesis
- RNA and protein synthesis mechanisms
- Hormonal Regulation and Hypertension
- Reproductive tract infections research
- Selenium in Biological Systems
- Microbial Fuel Cells and Bioremediation
- Effects and risks of endocrine disrupting chemicals
- DNA and Nucleic Acid Chemistry
- Water Treatment and Disinfection
- Cancer Research and Treatments
Shanghai Jiao Tong University
2016-2025
Cancer Research Center
2025
Xiamen University
2025
Quanzhou Normal University
2019
A gram-negative aliphatic hydrocarbon-degrading bacterium SJTD-1 isolated from oil-contaminated soil was identified as Pseudomonas aeruginosa by comparative analyses of the 16S rRNA sequence, phenotype, and physiological features. could efficiently mineralize medium- long-chain n-alkanes (C12-C30) its sole carbon source within seven days, showing most optimal growth on n-hexadecane, followed n-octadecane, n-eicosane. In 36 h, 500 mg/L tetradecane, hexadecane, octadecane were transformed...
Transmembrane alkane monooxygenase (AlkB)-type monooxygenases, especially AlkB2 are crucial for aerobic degradation of the medium-to-long-chain n-alkanes in hydrocarbon-utilizing microorganisms. In this study, we identified a GntR/VanR transcription regulator AlkR Pseudomonas aeruginosa SJTD-1 involved negative regulation and deciphered its nature DNA binding ligand release. The deletion alkR enhanced levels alkB2 gene utilization efficiency by strain SJTD-1. dimer recognizes binds to...
The lambda Red recombination system has been used to inactivate chromosomal genes in various bacteria and fungi. procedure consists of electroporating a polymerase chain reaction (PCR) fragment containing antibiotic cassette flanked by homology regions the target locus into strain that can express proteins (Gam, Bet, Exo). Here scarless gene modification strategy based on developed modify Pseudomonas genome DNA via sequential deletion multiple targets. This process was mediated plasmid...
Pseudomonas putida strain SJTE-1 can utilize 17β-estradiol and other environmental estrogens/toxicants, such as estrone, naphthalene sole carbon sources. We report the draft genome sequence of (5,551,505 bp, with a GC content 62.25%) major findings from its annotation, which could provide insights into biodegradation mechanisms.
Abstract Microorganism degradation is efficient to remove the steroid hormones like 17β-estradiol (E2); but their mechanism and metabolic network these chemicals are still not very clear. Here global responses of estrogen-degradation strain Pseudomonas putida SJTE-1 glucose were analyzed compared using iTRAQ (isobaric tags for relative absolute quantization) strategy combined with LC-MS/MS (liquid chromatography-tandem mass spectrometry). 78 proteins identified significant changes in...
Tellurite is highly toxic to bacteria and commonly used in the clinical screening for pathogens; it speculated that there a potential relationship between tellurite resistance bacterial pathogenicity. Until now, core function genes of their characteristics are still obscure. Pseudomonas citronellolis SJTE-3 was found able resist high concentrations (250 μg/mL) formed vacuole-like tellurium nanostructures. The terZABCDE gene cluster located large plasmid pRBL16 endowed strain with levels....
Streptomyces are well known for their potential to produce various pharmaceutically active compounds, the commercial development of which is often limited by low productivity and purity desired compounds expressed natural producers. Well-characterized promoters crucial driving expression target genes improving production metabolites interest.A strong constitutive promoter, stnYp, was identified in flocculus CGMCC4.1223 characterized its effective activation silent biosynthetic high...
ABSTRACT Pseudomonas citronellolis SJTE-3, isolated from the active sludge of a wastewater treatment plant in China, can utilize series environmental estrogens and estrogen-like toxicants. Here, we report its whole-genome sequence, containing one circular chromosome plasmid. Genes involved estrogen biodegradation this bacterium were predicted.
Polycyclic aromatic hydrocarbons (PAHs) are common organic pollutants with great carcinogenic threaten, and metal/PAH-contaminated environments represent one of the most difficult remedial challenges. In this work, Sphingobium yanoikuyae SJTF8 was isolated identified stable PAH-degrading efficiency even under stress conditions. It could utilize typical PAHs (naphthalene, phenanthrene, anthracene) heterocyclic halogenated compounds (dibenzothiophene 9-bromophenanthrene) as sole carbon source....
Pseudomonas aeruginosa strain SJTD-1 can utilize long-chain alkanes, diesel oil, and crude oil as sole carbon sources. We report the draft genome sequence of (6,074,058 bp, with a GC content 66.83%) major findings from its annotation, which could provide insights into petroleum biodegradation mechanism.
Abstract Modulation of protein function through allosteric regulation is central in biology, but biomacromolecular systems involving multiple subunits and ligands may exhibit complex regulatory mechanisms at different levels, which remain poorly understood. Here, we discover an aldo-keto reductase termed AKRtyl present its three-level mechanism. Specifically, by combining steady-state transient kinetics, X-ray crystallography molecular dynamics simulation, demonstrate that exhibits a...
Bufadienolides are a class of steroids with distinctive α-pyrone ring at C17, mostly produced by toads and consisting over 100 orthologues. They exhibit potent cardiotonic antitumor activities active ingredients the traditional Chinese medicine Chansu Cinobufacini. Direct extraction from is costly, chemical synthesis difficult, limiting accessibility bufadienolides diverse modifications trace content. In this work, based on transcriptome genome analyses, using yeast-based screening platform,...
AlkB monooxygenases in bacteria are responsible for the hydroxylation of medium- and long-chain n-alkanes. In this study, one CrgA protein Pseudomonas aeruginosa SJTD-1, a member LysR family, was proved to regulate AlkB2 monooxygenase degradation medium-to-long-chain n-alkanes (C14-C20) by directly binding upstream alkB2 gene. Two specific sites were found promoter region gene, imperfect mirror repeat (IIR) structure critical recognition binding. Hexadecyl CoA octadecyl could effectively...