A5101464601- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Biosensors and Analytical Detection
- Carbon and Quantum Dots Applications
- Inflammasome and immune disorders
- RNA modifications and cancer
- Biomedical Text Mining and Ontologies
- Extracellular vesicles in disease
- Gout, Hyperuricemia, Uric Acid
- Bacteriophages and microbial interactions
- Pulmonary Hypertension Research and Treatments
- Advanced Nanomaterials in Catalysis
- Topic Modeling
- Eosinophilic Esophagitis
- Health, Environment, Cognitive Aging
- Apelin-related biomedical research
- Advanced materials and composites
- Autophagy in Disease and Therapy
- Lipid metabolism and disorders
- Metal and Thin Film Mechanics
- Protein Degradation and Inhibitors
- Advanced Glycation End Products research
- Tissue Engineering and Regenerative Medicine
- Dye analysis and toxicity
Tianjin University of Science and Technology
2023-2024
Guangzhou University
2024
Guangzhou University of Chinese Medicine
2022-2024
Key Laboratory of Guangdong Province
2024
Shandong University
2024
Washington University in St. Louis
2024
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
2021-2023
Collaborative Innovation Center of Advanced Microstructures
2021-2023
Nanjing University
2019-2023
Tianjin University of Traditional Chinese Medicine
2020-2022
Triple-negative breast cancer (TNBC) is highly aggressive with a poor clinical prognosis and no targeted therapy. The c-Myc protein master transcription factor potential therapeutic target for TNBC. In this study, we develop PROTAC (PROteolysis TArgeting Chimera) based on TNA (threose nucleic acid) DNA that effectively targets degrades c-Myc. aptamer selected in vitro to bind the c-Myc/Max heterodimer appended E-box sequence create high-affinity, biologically stable bivalent binder. TNA-E...
Threose nucleic acid (TNA) has been considered a potential RNA progenitor in evolution due to its chemical simplicity and base pairing property. Catalytic TNA sequences with ligase activities might have facilitated the transition world. Here we report isolation of enzymes by vitro selection. The identified enzyme T8-6 catalyzes formation 2′–5′ phosphoester bond between 2′,3′-diol 5′-triphosphate group, kobs 1.1 × 10–2 min–1 (40 mM Mg2+, pH 9.0). For efficient reaction, requires UA|GA at...
The inflammatory microenvironment after acute myocardial infarction (MI) is a key limiting factor in the clinical application of stem cell transplantation and paracrine exosome therapy. Qishen Yiqi Pills contain saponin ingredient called Ginsenoside Rh2 (Rh2) which exhibits certain therapeutic effect on MI. However, mechanism by alleviates improves efficiency exosomes remains enigmatic. Here, we found that attenuated adverse oxygen-glucose deprivation (OGD)-induced cellular injury, an vitro...
Cardiovascular, respiratory, and nervous system diseases have high morbidity mortality rates, but the causal relationship between air pollution these remains controversial.
Objective: To observe the effect of Si-Miao-Yong-An (SMYA) on atherosclerosis (AS) vulnerable plaques, and to further explore mechanism by vasa vasorum (VV) angiogenesis maturation as an entry point. Methods: SPF-class healthy male ApoE −/− mice were randomized into model group, simvastatin group SMYA C57BL/6 used control group. After 8 weeks intervention, pathological morphology plaque was observed HE staining; VV density in aortic adventitia immunohistochemistry; measured double-labelling...
Background: Myocardial ischemia/reperfusion (I/R) injury is associated with multiple serious clinical manifestations. Autophagy upregulated in a short period of ischemia and further enhanced during reperfusion phase, which was considered as "double-edged sword" the pathological process myocardial I/R injury. In addition, NLRP3 inflammasome triggers inflammatory response, leads to cardiomyocyte death via pyroptosis promotes subsequent remodelling. Qishen Yiqi Dripping Pill (QSYQ) has been...
QiShen YiQi pills (QSYQ) is a Traditional Chinese Medicine (TCM) formula, which has significant effect on the treatment of patients with myocardial infarction (MI) in clinical practice. However, molecular mechanism QSYQ regulation pyroptosis after MI still not fully known. Hence, this study was designed to reveal active ingredient QSYQ.Integrated approach network pharmacology and docking, were conducted screen components corresponding common target genes intervening MI. Subsequently, STRING...
Background Evidence from observational studies and clinical trials suggests that the allergic diseases (ADs) are associated with kidney (KDs). However, causal association between them remains to be determined. We used bidirectional two-sample Mendelian randomization (MR) analysis evaluate potential causality them. Methods was performed using publicly available genome-wide study (GWAS) summary datasets. Inverse variance weighted (IVW), median, MR-Egger regression, simple mode, mode methods...
Catalytic DNA-based fluorescent sensors have enabled cellular imaging of metal ions such as Mg2+ . However, natural DNA is prone to nuclease-mediated degradation. Here, we report the in vitro selection threose nucleic acid enzymes (TNAzymes) with RNA endonuclease activities. One TNAzyme, T17-22, catalyzes a site-specific cleavage reaction kcat 0.017 min-1 and KM 675 nM. A sensor based on T17-22 responds an increasing concentration limit detection at 0.35 mM. This TNAzyme-based also allows...
Threose nucleic acid (TNA) is considered a potential RNA progenitor due to its chemical simplicity, base pairing property, and capability of folding into functional tertiary structure. However, it unknown whether the property can be maintained during transition from TNA RNA. Here, we use toggle in vitro selection identify catalyst sequences that are active both backbones. One such enzyme with exchangeable backbone (CAMELEON) catalyzes an cleavage reaction when prepared as (T) (R). Further...
Si-Miao-Yong-An (SMYA) has been widely used for the clinical treatment of atherosclerosis (AS). Yet, its complete mechanism action is not fully understood.To investigate by which SMYA stabilizes AS plaques from perspective inhibiting vasa vasorum (VV) angiogenesis.We male ApoE-/- mice to establish an model. The were divided into model, (11.7 mg/kg/d), and simvastatin (SVTT) (2.6 mg/kg/d) groups. Mice given or SVTT daily gavage 8 weeks. HE staining, immunofluorescence double-labelling...
Abstract Deoxyribozymes capable of catalyzing sequence-specific RNA cleavage have found broad applications in biotechnology, DNA computing and environmental sensing. Among these, deoxyribozyme 8–17 is the most common small motif cleavage. However, extent to which other molecules with similar catalytic motifs exist remains elusive. Here we report a novel RNA-cleaving called 10–12opt that functions an equally unusually short binding arm. This contains 14-nucleotide core preferentially...
Clinical Decision Systems utilize patient profiles to search for relevant medical support. Existing work on has a primary topic query expansion, which enriches queries by adding more useful terms. However, it performs well when are concise. In this paper, we aim tackle verbose and propose new automatic reformulation method, not only considers but also includes reduction. This improves the searching performance refining effectively avoid irrelevant results. The method is achieved classifying...
<title>Abstract</title> Purpose It is clinically important to refine strategies manage women with dense breasts. We aim identify changes in density patterns related breast cancer risk. Methods This case-control study was sampled from the Joanne Knight Breast Health Cohort of 10,481 free at entry and followed November 3, 2008 October 31, 2020. Routine screening mammograms were performed every 1 2 years, providing a measure density. 289 pathology-confirmed cases identified approximately...