A5100425339- Nanopore and Nanochannel Transport Studies
- RNA modifications and cancer
- Microfluidic and Capillary Electrophoresis Applications
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Advancements in Battery Materials
- Nanoplatforms for cancer theranostics
- Cancer-related molecular mechanisms research
- Advanced Battery Materials and Technologies
- Bacteriophages and microbial interactions
- Glycosylation and Glycoproteins Research
- Lipid Membrane Structure and Behavior
- Fuel Cells and Related Materials
- Supercapacitor Materials and Fabrication
- Nanoparticle-Based Drug Delivery
- Genetic and phenotypic traits in livestock
- Advanced Battery Technologies Research
- MicroRNA in disease regulation
- Education and Work Dynamics
- Circular RNAs in diseases
- Genomics and Phylogenetic Studies
- Higher Education and Teaching Methods
- Cancer, Hypoxia, and Metabolism
- Biofuel production and bioconversion
- Advanced Nanomaterials in Catalysis
Shaanxi Normal University
2025
Southwest Forestry University
2023-2025
Fujian Provincial Cancer Hospital
2025
Fudan University Shanghai Cancer Center
2025
Fujian Medical University
2025
First People's Hospital of Chongqing
2025
Nanjing University
2011-2024
Harbin Institute of Technology
2020-2024
Third Affiliated Hospital of Sun Yat-sen University
2023-2024
Sun Yat-sen University
2022-2024
Diverse functions of proteins, including synthesis, catalysis, and signaling, result from their highly variable amino acid sequences. The technology allowing for direct analysis protein sequences, however, is still unsatisfactory. Recent developments nanopore sequencing DNA or RNA have motivated attempts to realize peptides in a similar manner. core challenge has been achieve controlled ratcheting motion the target peptide, which currently restricted limited choice compatible enzymes. By...
Abstract Nanopore direct RNA sequencing (DRS) has emerged as a powerful tool for modification identification. However, concurrently detecting multiple types of modifications in single DRS sample remains challenge. Here, we develop TandemMod, transferable deep learning framework capable data. To train high-performance TandemMod models, generate vitro epitranscriptome datasets from cDNA libraries, containing thousands transcripts labeled with various modifications. We validate the performance...
Abstract Brown adipose tissue (BAT) undergoes rapid postnatal development and then protects against cold obesity into adulthood. However, the molecular mechanism that determines maturation of BAT is largely unknown. Here we show METTL3 (a key RNA methyltransferase) expression increases significantly in interscapular brown (iBAT) after birth plays an essential role iBAT. BAT-specific deletion Mettl3 severely impairs vivo by decreasing m 6 A modification Prdm16 , Pparg Ucp1 transcripts, which...
Abstract Saccharides play critical roles in many forms of cellular activities. Saccharide structures are however complicated and similar, setting a technical hurdle for direct identification. Nanopores, which emerging single molecule tools sensitive to minor structural differences between analytes, can be engineered identity saccharides. A hetero‐octameric Mycobacterium smegmatis porin nanopore containing phenylboronic acid was prepared, able clearly identify nine monosaccharide types,...
Abstract Folding of RNA can produce elaborate tertiary structures, corresponding to their diverse roles in the regulation biological activities. Direct observation structures at high resolution native form however remains a challenge. The large vestibule and narrow constriction Mycobacterium smegmatis porin A (MspA) suggests sensing mode called nanopore trapping/translocation, which clearly distinguishes between microRNA, small interfering (siRNA), transfer (tRNA) 5 S ribosomal (rRNA). To...
The nanopore is emerging as a means of single-molecule protein sensing. However, proteins demonstrate different charge properties, which complicates the design sensor that can achieve simultaneous sensing differently charged proteins. In this work, we introduce an asymmetric electrolyte buffer combined with Mycobacterium smegmatis porin A (MspA) to form electroosmotic flow (EOF) trap. Apo- and holo-myoglobin, differ in only single heme, be fully distinguished by method. Direct discrimination...
Alditols, which have a sweet taste but produce much lower calories than natural sugars, are widely used as artificial sweeteners. Alditols the reduced forms of monosaccharide aldoses, and different alditols diastereomers or epimers each other direct rapid identification by conventional methods is difficult. Nanopores, emerging single-molecule sensors with exceptional resolution when engineered appropriately, useful for recognition epimers. In this work, distinguishing corresponding to all 15...
Biomaterial-based implants hold great potential for postoperative cancer treatment due to the enhanced drug dosage at disease site and decreased systemic toxicity. However, elaborate design of avoid complicated chemical modification burst release remains challenging. Herein, we report a three-dimensional (3D) printed hydrogel scaffold enable sustained drugs synergistic therapy. The is composed Pluronic F127 sodium alginate (SA) as well doxorubicin (DOX) copper ions (F127-SA/Cu-DOX scaffold)....
Abstract Natural herbs, which contain pharmacologically active compounds, have been used historically as medicines. Conventionally, the analysis of chemical components in herbal medicines requires time-consuming sample separation and state-of-the-art analytical instruments. Nanopore, a versatile single molecule sensor, might be suitable to identify bioactive compounds natural herbs. Here, phenylboronic acid appended Mycobacterium smegmatis porin A (MspA) nanopore is sensor for variety based...
Abstract Natural fruits contain a large variety of cis -diols. However, due to the lack high-resolution sensor that can simultaneously identify all -diols without need complex sample pretreatment, direct and rapid analysis in hand-held device has never been previously reported. Nanopore, versatile single molecule sensor, be specially engineered perform this task. A hetero-octameric Mycobacterium smegmatis porin (MspA) nanopore modified with sole phenylboronic acid (PBA) adapter is prepared....
Abstract Although GPR3 plays pivotal roles in both the nervous system and metabolic processes, such as cold-induced thermogenesis, its endogenous ligand remains elusive. Here, by combining structural approach (including cryo-electron microscopy), mass spectrometry analysis, functional studies, we identify oleic acid (OA) an of GPR3. Our study reveals a hydrophobic tunnel within that connects extracellular side receptor to middle plasma membrane, enabling fatty acids readily engage receptor....
Up-regulation of core fucosylation catalyzed by α1,6-fucosyltransferase (Fut8) has been observed in hepatocellular carcinoma (HCC). Here, to explore the role Fut8 expression hepatocarcinogensis, we established chemical-induced HCC models male wild-type (WT; Fut8+/+), hetero (Fut8+/-), and knockout (KO; mice use diethylnitrosamine (DEN) pentobarbital (PB). In Fut8+/+ Fut8+/- mice, multiple large vascularized nodules were induced with an increased after DEN PB treatment. However, formation...
Biological nanopores are capable of resolving small analytes down to a monoatomic ion. In this research, tetrachloroaurate(III), polyatomic ion, is discovered bind the methionine residue (M113) wild-type α-hemolysin by reversible Au(III)-thioether coordination. However, cylindrical pore geometry generates shallow ionic binding events (~5-6 pA) and may have introduced other undesired interactions. Inspired nanopore sequencing, Mycobacterium smegmatis porin A (MspA) nanopore, which possesses...
The principle of hard–soft-acid–base (HSAB) theory was first validated in single molecule by measurements with engineered<italic>Mycobacterium smegmatis</italic>porin A (MspA) nanopore reactors.
Enantiomers, chiral isomers with opposite chirality, typically demonstrate differences in their pharmacological activity, metabolism, and toxicity. However, direct discrimination between enantiomers is challenging due to similar physiochemical properties. Following the strategy of programmable nanoreactors for stochastic sensing (PNRSS), introduction phenylboronic acid (PBA) a Mycobacterium smegmatis porin A (MspA) assists identification norepinephrine epinephrine. Using machine learning...
A new biphasic organic solvent, phenoxyethanol-maleic acid, was carried out to pretreat and fractionate vinegar residue into glucan, xylan lignin under mild conditions. Additional effects of key factors, temperature phenoxyethanol concentration, on residue, were evaluated. Under the system (0.5% maleic 60% phenoxyethanol), 140 °C cooking for 1 h, 80.91% cellulose retention in solid 75.44% hemicellulose removal 69.28% obtained. Optimal identified conditions resulted maximum XOS 47.3%. Then,...