A5006956435- Microbial Natural Products and Biosynthesis
- Natural product bioactivities and synthesis
- Plant biochemistry and biosynthesis
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Bioactive natural compounds
- Chemical Synthesis and Analysis
- Biological Activity of Diterpenoids and Biflavonoids
- Luminescence and Fluorescent Materials
- Supramolecular Chemistry and Complexes
- Biochemical and biochemical processes
- Fungal Biology and Applications
- Computational Drug Discovery Methods
- COVID-19 Clinical Research Studies
- Phytochemicals and Antioxidant Activities
- Microbial Metabolism and Applications
- Molecular spectroscopy and chirality
- Molecular Sensors and Ion Detection
- Crystallography and molecular interactions
- Metal-Organic Frameworks: Synthesis and Applications
- Receptor Mechanisms and Signaling
- Chromatography in Natural Products
- Plant Gene Expression Analysis
- Plant Toxicity and Pharmacological Properties
- Monoclonal and Polyclonal Antibodies Research
Central South University
2016-2025
Beijing Chest Hospital
2023-2025
Capital Medical University
2023-2025
McGill University
2025
Tsinghua University
2022-2024
Jiangxi Normal University
2024
Second Affiliated Hospital of Guangzhou Medical University
2024
Guangzhou Medical University
2024
East China University of Science and Technology
2024
Guizhou University
2018-2021
Abstract Although GPR3 plays pivotal roles in both the nervous system and metabolic processes, such as cold-induced thermogenesis, its endogenous ligand remains elusive. Here, by combining structural approach (including cryo-electron microscopy), mass spectrometry analysis, functional studies, we identify oleic acid (OA) an of GPR3. Our study reveals a hydrophobic tunnel within that connects extracellular side receptor to middle plasma membrane, enabling fatty acids readily engage receptor....
Nuclear magnetic resonance (NMR) chemical shift calculations are powerful tools for structure elucidation and have been extensively employed in both natural product synthetic chemistry. However, density functional theory (DFT) NMR usually time-consuming, while fast data-driven methods often lack reliability, making it challenging to apply them computationally intensive tasks with a high requirement on quality. Herein, we constructed 54-layer-deep graph convolutional network
Energy transfer and interchange are central for fabricating white light-emitting organic materials. However, increasing the efficiency of light energy remains a considerable challenge because occurrence "cross talk". In this work, by exploiting unique photophysical properties cucurbituril-triggered host–guest interactions, two complementary luminescent colors blue yellow emission were independently obtained from single fluorophore dye rather than transfer. Further study suggested that rigid...
Abstract Leukotriene B4 receptor 1 (BLT1) plays crucial roles in the acute inflammatory responses and is a valuable target for anti-inflammation treatment, however, mechanism by which leukotriene (LTB4) activates remains unclear. Here, we report cryo-electron microscopy (cryo-EM) structure of LTB4 -bound human BLT1 complex with G i protein an active conformation at resolution 2.91 Å. In combination molecule dynamics (MD) simulation, docking site-directed mutagenesis, our reveals that...
The putative prenyltransferase gene ACLA_031240 belonging to the dimethylallyltryptophan synthase superfamily was identified in genome sequence of Aspergillus clavatus and overexpressed Escherichia coli. soluble His-tagged protein EAW08391 purified near homogeneity used for biochemical investigation with diverse aromatic substrates presence different prenyl diphosphates. It has shown that dimethylallyl diphosphate (DMAPP), recombinant enzyme accepted very well simple indole derivatives...
Nature synthesizes many strained natural products that have diverse biological activities. Uncovering these biosynthetic pathways may lead to biomimetic strategies for organic synthesis of such compounds. In this work, we elucidated the concise pathway herquline A, a highly and reduced fungal piperazine alkaloid. The builds on nonribosomal peptide synthetase derived dityrosine intermediate. Following enzymatic reduction P450-cross-linked dicyclohexadienone, N-methylation serves as trigger...
Six β-configured reversely C3-prenylated pyrrolo[2,3-b]indoles were successfully prepared by using a recombinant prenyltransferase from Neosartorya fischeri. For this purpose, the putative gene NFIA_074280 (termed herewith cdpC3PT) was cloned into pQE60 and overexpressed in Escherichia coli. The overproduced His6-CdpC3PT purified to near homogeneity incubated with five cyclic tryptophan-containing dipeptides presence of dimethylallyl diphosphate (DMAPP). All substrates accepted CdpC3PT...
The CorA family of divalent cation transporters utilizes Mg2+ and Co2+ as primary substrates. molecular mechanism its function, including ion selectivity gating, has not been fully characterized. Recently we reported a new structure homologue from Methanocaldococcus jannaschii, which provided novel structural details that offered the conception unique gating involving conversion an open hydrophilic gate into closed hydrophobic one. In present study report functional evidence for this in...
The NADase activity of the defense-associated sirtuins (DSRs) is activated by phage tail tube protein (TTP). Herein, we report cryo-EM structures a free-state Bacillus subtilis DSR2 tetramer and fragment tetramer, SPR tube, two DSR2-TTP complexes. contains an N-terminal SIR2 domain, middle domain (MID) C-terminal (CTD). CTD harbors α-solenoid tandem-repeats like HEAT-repeat proteins. assembles into with four clustered at center, intertwined MID-CTD chains flank core. TTPs self-assemble...
Production of typical plant metabolites by a fungal enzyme: Fungal prenyltransferases the DMATS superfamily are mainly involved in biosynthesis prenylated indole alkaloids, but also catalyze prenylation tyrosine and naphthalene derivatives. In this study, nine flavonoids were produced using recombinant dimethylallyltryptophan synthase 7-DMATS from Aspergillus fumigatus.
Mycoplasma pneumoniae (MP) has recently become an important pathogen of respiratory infection in both children and adults. The objective this study was to investigate the detection rate MP clinical features viral coinfection pneumonia (MPP) after coronavirus disease 2019 (COVID-19) pandemic southern Guangzhou. We reviewed 3,094 patients with who visited medicine pediatrics department from October 2023 January 2024. Patients underwent polymerase chain reaction (PCR) or immunoglobulin M (IgM)...
This retrospective analysis examines the local control and toxicity of five-fraction fiducial-free CyberKnife stereotactic body radiation therapy (SBRT) for single vertebral (VB) metastases. All patients had favorable performance status (ECOG 0-1), oligometastatic disease, no prior spine irradiation. A prescribed dose 30-35 Gy was delivered in five fractions to planning target volume (PTV) using with X-sight tracking. Suggested maximum spinal cord esophagus point doses were 30 40 Gy,...
Flavonoids are found mainly in plants and exhibit diverse biological pharmacological activities, which can often be enhanced by prenylations. In plants, such reactions catalyzed membrane-bound prenyltransferases. this study, the prenylation of nine flavonoids from different classes a soluble fungal prenyltransferase (AnaPT) involved biosynthesis prenylated indole alkaloid acetylaszonalenin is demonstrated. The behavior AnaPT toward regarding substrate acceptance positions clearly differs...