A5101408505- Metabolomics and Mass Spectrometry Studies
- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Atrial Fibrillation Management and Outcomes
- Nanoplatforms for cancer theranostics
- Bladder and Urothelial Cancer Treatments
- Diet and metabolism studies
- Venous Thromboembolism Diagnosis and Management
- Metabolism and Genetic Disorders
- Blood Pressure and Hypertension Studies
- Gut microbiota and health
- RNA Interference and Gene Delivery
- Photodynamic Therapy Research Studies
- Dendrimers and Hyperbranched Polymers
- Skin Protection and Aging
- Tryptophan and brain disorders
- Bone Metabolism and Diseases
- Medicinal Plants and Bioactive Compounds
- Olfactory and Sensory Function Studies
- Cardiovascular Issues in Pregnancy
- Sulfur Compounds in Biology
- Urinary and Genital Oncology Studies
- Chromatography in Natural Products
- Gestational Diabetes Research and Management
- Advanced Proteomics Techniques and Applications
Capital Medical University
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2025
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2022
Peking Union Medical College Hospital
2021
Academy of Medical Sciences
2021
Wenzhou Medical University
2021
Shenyang Pharmaceutical University
2009-2020
Qingdao University
2004-2017
Urine metabolomics have been used to identify biomarkers for clinical diseases. However, inter‐individual variations and effect factors need be further evaluated. In our study, we explored the urine metabolome in a cohort of 203 health adults, 6 patients with benign bladder lesions, 53 cancer (BCa) using liquid chromatography coupled high resolution mass spectrometry. Inter‐individual analysis both healthy controls BCa showed that was relatively stable. Further indicated sex age affect...
Bladder cancer (BC) and Renal cell carcinoma(RCC) are the two most frequent genitourinary cancers in China. In this study, a comprehensive liquid chromatography-mass spectrometry (LC-MS) based method, which utilizes both plasma metabolomics lipidomics platform, has been carried out to discriminate global profiles of 64 patients with BC, 74 RCC, 141 healthy controls. Apparent separation was observed between (BC RCC) samples The area under receiving operator characteristic curve (AUC) 0.985...
Abstract Tumor-derived exosomes are important for cell-cell communication. However, the role of TP53 in control exosome production colorectal cancer (CRC) is controversial and unclear. The features secreted from HCT116 -wild type (WT), -knockout (KO) constructed (R273H)-mutant (MT) cells were assessed. MT KO exhibited significantly reduced sizes compared with WT cells. A comprehensive proteomic analysis exosomal proteins was performed using isobaric tag relative absolute quantitation...
Background Clinical outcomes of bladder cancer (BC) is tightly associated with the stage and grade initial diagnosed BC so that early detection clearly important for patients BC. However, diagnostic capability current detective methods, such as urinary cytology, cystoscopy, imageology method several urine-based tests, insufficient. The objective our study to discover novel biomarkers BC, called non-muscle invasive (NMI) using liquid chromatography-high resolution mass spectrometry...
Renal cell carcinoma (RCC) is the second most lethal urinary cancer. RCC frequently asymptomatic and it already metastatic at diagnosis. There an urgent necessity for specific biomarkers selection diagnostic prognostic purposes. In present study, we applied liquid chromatography-mass spectrometry (LC-MS) based metabolomics to analyze urine samples of 100 RCC, 34 benign kidney tumors 129 healthy controls. Differential metabolites were analyzed investigate if could differentiate from non-RCC....
Abstract Urinary proteomics is emerging as a potent tool for detecting sensitive and non-invasive biomarkers. At present, the comparability of urinary data across diverse liquid chromatography−mass spectrometry (LC-MS) platforms remains an area that requires investigation. In this study, we conduct comprehensive evaluation proteome multiple LC-MS platforms. To systematically analyze assess quality large-scale data, develop control (QC) system named MSCohort, which extracted 81 metrics...
Brainstem gliomas (BSGs) harboring a histone 3 lysine27-to-methionine (H3K27M) mutation represent one of the deadliest brain tumors with dismal prognosis, as they exhibit much worse response to therapy compared wildtype BSGs. Early non-invasive recognition H3K27M is paramount for clinical decision-making in treating Plasma and urine samples were prospectively collected from BSG patients before biopsy or surgical resection chronologically divided into discovery, test, validation cohorts....
Acute coronary syndrome (ACS) is a cardiovascular disease caused by acute myocardial ischemia. The aim of this study was to use urine metabolomics explore potential biomarkers for the diagnosis ACS and changes in metabolites during development disease. Urine samples were collected from 81 healthy controls 130 patients (103 UA 27 AMI). Metabolomics based on liquid chromatography-mass spectrometry (LC-MS) used analyze samples. Statistical analysis functional annotation applied identify...
Reactive oxygen species (ROS)-based photodynamic therapy (PDT) has a widespread application in cancer therapy.Nevertheless, the efficiency of PDT is far from satisfactory.One major impediment overexpression glutathione (GSH) tumor cells, which could deplete level PDT-generated ROS.Herein, we develop novel type cytochrome P450 enzyme-mediated auto-enhanced co-nanoassembly between clopidogrel (CPG) and photosensitizer pyropheophorbide (PPa).Methods: In this work, prepare co-assembled...
A temperature-sensitive hydrogel encapsulating tegafur and protoporphyrin IX dimers could be delivered intratumorally for synergetic chemotherapy photodynamic therapy.
Pregnancy is associated with the onset of many adaptation processes that are likely to change over course gestation. Understanding normal metabolites' variation pregnancy progression crucial for gaining insights key nutrients fetal growth, and comparative research pregnancy-related complications. This work presents liquid chromatography-mass spectrum-based urine metabolomics study 50 health pregnant women at three time points during pregnancy. The influence maternal physiological factors,...
Background The saliva metabolome has been applied to explore disease biomarkers. In this study we characterized the metabolic profile of primary Sjögren’s syndrome (pSS) patients and explored metabolomic Methods This work presents a liquid chromatography-mass spectrometry-based 32 with pSS 38 age- sex-matched healthy adults. Potential metabolite biomarkers were using test group samples (20 vs. 25 adults) then verified by cross-validation (12 13 adults). Results Metabolic pathways, including...
To discover biomarker panels that could distinguish cancers (BC and RCC) from healthy controls (HCs) bladder (BC) renal cell carcinoma (RCC), regardless of whether the patients have haematuria. In addition, we also explored altered metabolomic pathways BC RCC.In total, 403 participants were enrolled in our study, which included 146 (77 without haematuria 69 with haematuria), 115 RCC (94 21 haematuria) 142 sex- age-matched HCs. Their midstream urine samples collected analysed by performing...
Curcumin is a natural polyphenol extracted from the turmeric rhizome, which has wide range of biological activities, but until now effects curcumin on gastrointestinal peristalsis have not been fully understood. In vivo study, we observed gastric emptying and intestinal propulsion rates mice in normal state delayed by atropine (ATR) or nitric oxide precursor L-arginine (L-Arg). An vitro study explored direct contractility, were studied through measuring spontaneous contraction isolated...
Background Colorectal Cancer (CRC) is a prevalent digestive system tumour with significant mortality and recurrence rates. Serum metabolomics, its high sensitivity throughput, has shown potential as tool to discover biomarkers for clinical screening monitoring of the CRC patients. Methods metabolites 61 sex age-matched healthy controls 62 patients (before after surgical intervention) were analyzed using ultra-performance liquid chromatography-high resolution mass spectrometer (UPLC-MS)....
Abstract Urinary metabolomics is a useful non-invasive tool for large-scale screening of disease-related metabolites. However, no comprehensive urinary metabolomic analysis vitiligo presently available. To investigate the urine metabolic pattern patients, we conducted combined cross-sectional and prospective self-control cohort study an untargeted analysis. In study, 295 patients 192 age‐ sex‐matched controls were enrolled, 71 differential metabolites between two groups identified. Pathway...
Mucin 1 (MUC1) has been regarded as an ideal target for cancer treatment, since it is overexpressed in a variety of different cancers including the majority breast cancer. However, there are still no approved monoclonal antibody drugs targeting MUC1. In this study, we generated humanized MUC1 (HzMUC1) from our previously developed mouse that only recognizes on surface tumor cells. Furthermore, antibody-drug conjugate (ADC) was by conjugating HzMUC1 with monomethyl auristatin (MMAE), and...
Urine metabolomics has been a promising technique in the liquid biopsy of urothelial cancer (UC). The comparison upper tract (UTUC), lower (BCa), and healthy controls (HCs) need to be performed find related biomarkers.In our investigation, urine samples from 35 UTUCs, 44 BCas, 53 gender- age-matched HCs were analyzed using chromatography-high resolution mass spectrometry (LC-HRMS). In different groups, differential metabolites disturbed metabolism pathways explored. Transcriptomics are...
Plasma metabolomics and lipidomics have been commonly used for biomarker discovery. Studies in white Japanese populations suggested that gender age can affect circulating plasma metabolite profiles; however, the characteristics Chinese population has not surveyed. In our study, we applied liquid chromatography-mass spectrometry-based approach to analyze metabolome lipidome a cohort of 534 healthy adults (aging from 15 79). Fatty-acid metabolism was found be gender- age-dependent Chinese,...