A5115602047- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Microtubule and mitosis dynamics
- RNA modifications and cancer
- Cancer Research and Treatments
- Dental Implant Techniques and Outcomes
- Cancer-related gene regulation
- Genomics and Phylogenetic Studies
- Cancer-related Molecular Pathways
- Craniofacial Disorders and Treatments
- Cleft Lip and Palate Research
- Molecular Biology Techniques and Applications
- Cancer-related molecular mechanisms research
- Virus-based gene therapy research
- Cancer, Hypoxia, and Metabolism
- Orthodontics and Dentofacial Orthopedics
- Nanoparticle-Based Drug Delivery
- Nerve injury and regeneration
- Dendrimers and Hyperbranched Polymers
- Actinomycetales infections and treatment
- Endoplasmic Reticulum Stress and Disease
- Hippo pathway signaling and YAP/TAZ
- Ubiquitin and proteasome pathways
- Histone Deacetylase Inhibitors Research
- Neurogenesis and neuroplasticity mechanisms
Shanxi Medical University
2013-2024
Chongqing Cancer Hospital
2021-2024
Chongqing University
2021-2024
Jilin University
2017-2024
Shanghai Jiao Tong University
2021-2024
Sichuan University
2014-2024
Chengdu University of Technology
2024
Xiamen University
2006-2024
Stomatology Hospital
2015-2024
Buchang Pharma (China)
2024
Efficient endosomal escape is the most essential but challenging issue for siRNA drug development. Herein, a series of quaternary ammonium-based amphiphilic triblock polymers harnessing an elaborately tailored pH-sensitive hydrophobic core were synthesized and screened. Upon incubating in pH environment (pH 6.5–6.8), mPEG45-P(DPA50-co-DMAEMA56)-PT53 (PDDT, optimized polymer) nanomicelles (PDDT-Ms) PDDT-Ms/siRNA polyplexes rapidly disassembled, leading to promoted cytosolic release...
mRNA is a novel class of therapeutic modality that holds great promise in vaccination, protein replacement therapy, cancer immunotherapy, immune cell engineering etc. However, optimization molecules and efficient vivo delivery are quite important but challenging for its broad application. Here we present an ionizable lipid nanoparticle (iLNP) based on iBL0713 vitro expression desired proteins using codon-optimized mRNAs. mRNAs encoding luciferase or erythropoietin (EPO) were prepared by...
The extremely low efficient cytosolic release of the internalized siRNA has emerged recently as a central issue for delivery, while there is lack guidelines to facilitate siRNA. To address these concerns, we studied contribution pH-sensitive inner core on handling delivered by series PG-P(DPAx-co-DMAEMAy)-PCB amphiphilic polycation nanomicelles (GDDC-Ms) with internalization (<1/4 lipofactamine 2000 (Lipo2000)). Significantly, just varying mole ratio DPA and DMAEMA adjust initial disassembly...
Pancreatic cancer is currently one of the deadliest solid malignancies, whose incidence and death rates are increasing consistently during past 30 years. Ribonucleotide reductase (RR) a rate-limiting enzyme that catalyzes formation deoxyribonucleotides from ribonucleotides, which essential for DNA synthesis replication. In this study, 23 small interfering RNAs (siRNAs) against RRM2, second subunit RR, were designed screened, them (termed siRRM2), with high potency good RNase-resistant...
The pharmacokinetics of small interfering RNAs (siRNAs) is a pivotal issue for siRNA-based drug development.In this study, we comprehensively investigated the behavior siRNAs in vivo various tissues and demonstrated that intravenously-injected naked siRNA accumulated remarkably submandibular gland, bulbourethral pancreas, with respective half-life ~22.7, ~45.6, ~30.3 h.This was further confirmed by gel separation tissue homogenates and/or supernatants.In imaging cryosectioning suggested...
The degeneration of nigral dopamine neurons in Parkinson's disease (PD) reportedly involves a defect brain mitochondrial complex I association with the activation nuclear factor-kappaB (NF-kappaB) and caspase-3. To elucidate molecular mechanisms possibly linking these events, as well to evaluate neuroprotective potential cyclopentenone prostaglandin A1 (PGA1), an inducer heat shock proteins (HSPs), we exposed human dopaminergic SH-SY5Y cells inhibitor rotenone. Dose-dependent apoptosis was...
Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive cancers, and metastasis principal cause death in ESCC patients. It has been shown that amplification overexpression mitotic serine/threonine kinase Aurora-A occur several types human tumors, including ESCC. Moreover, increase expression levels predicted to correlate with grades tumor differentiation invasive capability. However, mechanisms by which mediates its effects still remain elusive. In this article, we showed...
In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from faeces of Tibetan antelopes at Hoh Xil Nature Reserve, Qinghai-Tibet Plateau, China. The cells were aerobic, oxidase-negative catalase-positive. When cultured on brain-heart infusion agar supplemented with 5 % sheep blood, colonies cream in colour, circular, smooth convex. Phylogenetic analysis based full-length 16S rRNA sequences revealed type...
Abstract Continuous cell electroporation is an appealing non-viral approach for genetically transfecting a large number of cells. Yet the traditional macro-scale devices suffer from unsatisfactory transfection efficiency and/or viability due to their high voltage, while emerging microfluidic still limited by low processing speed. Here we present flow-through device integrating large-sized flow tube and small-spaced distributed needle electrode array. Relatively enables rate, simple...
Tanshinone IIA (TSN) exhibits a variety of anticancer effects. However, whether it inhibits gastric cancer (GC) cell proliferation and migration the mechanism remain unclear. In present study, different concentrations TSN were co-incubated with SGC-7901 cells. The pcDNA-FOXM1 or FOXM1-siRNA plasmid was transfected into cells before treatment 5 µg/l TSN. abilities tested by MTT wound healing assays. Western blotting used to investigate expression levels P21, Ki-67, PCNA, MMP-2, MMP-9 FOXM1....
Dual-energy computed tomography (DECT) is widely used to characterize and differentiate tumors. However, data regarding its diagnostic performance for the characterization of breast tumors are limited. In this study, we assessed quantitative parameters derived from DECT in differentiating benign malignant lesions predicting histopathological molecular subtypes patients with cancer.Dual-phase contrast-enhanced thorax was performed on participants Conventional CT attenuation parameters,...
Centrosome stability is required for successful mitosis in mammalian cells. Amplification of the centrosome leads to chromosomal missegregation and generation aneuploidy, which are closely associated with cell transformation tumorigenesis (Doxsey, S. J. (2001) Nat. Cell Biol. 3, E105-E108; Hinchcliffe, E. H., Sluder, G. Genes Dev. 15, 1167-1181; Pihan, A., Purohit, Wallace, J., Malhotra, R., Liotta, L., Doxsey, Cancer Res. 61, 2212-2219). However, there currently limited insights into...
Botanicals are rich in bioactive compounds, and some offer numerous beneficial effects to animal human health when consumed. It is well known that phytochemicals cranberries have anti-oxidative antimicrobial activities. Recently, an increasing body of evidence has demonstrated cranberry may potential benefits promote healthy aging. Here, we use Caenorhabditis elegans as a model show water-soluble extract standardized 4.0% proanthocyanidins (WCESP), major component cranberries, can enhance...
Small interfering RNA (siRNA) therapies have been hampered by lack of delivery systems in the past decades.Nowadays, a few promising vehicles for siRNA developed and it is gradually revealed that enhancing release from endosomes into cytosol very important factor successful delivery.Here, we designed novel pH-sensitive nanomicelle, PEG-PTTMA-P(GMA-S-DMA) (PTMS), delivery.Owing to rapid hydrolysis acidic environment, PTMS NPs underwent hydrophobic-to-hydrophilic transition enabled combination...