A5031654301- Identification and Quantification in Food
- Streptococcal Infections and Treatments
- Genetics, Aging, and Longevity in Model Organisms
- Biosensors and Analytical Detection
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Vector-borne infectious diseases
- Mosquito-borne diseases and control
- Plant Pathogenic Bacteria Studies
- Advanced biosensing and bioanalysis techniques
- Neonatal and Maternal Infections
- Antimicrobial Resistance in Staphylococcus
- Viral Infections and Vectors
- Aquaculture disease management and microbiota
- RNA and protein synthesis mechanisms
- Environmental DNA in Biodiversity Studies
- Listeria monocytogenes in Food Safety
- Infections and bacterial resistance
- Bee Products Chemical Analysis
- Vibrio bacteria research studies
- Salmonella and Campylobacter epidemiology
- Gut microbiota and health
- Bacterial biofilms and quorum sensing
- Circadian rhythm and melatonin
- Insect and Pesticide Research
Nanjing Tech University
2019-2025
Shanghai Ocean University
2025
Shanghai Academy of Agricultural Sciences
2025
Clemson University
2009-2024
Lanzhou University
2019
Huadong Hospital
2006-2018
Nanjing General Hospital of Nanjing Military Command
2005-2015
National Academy of Medicine
2013-2014
Jiangsu Province Blood Center
2014
Xian Yang Central Hospital
2012
Summary Bacillus subtilis encodes seven extracytoplasmic function (ECF) sigma factors. The σ W regulon includes functions involved in detoxification and protection against antimicrobials, whereas M is essential for growth at high salt concentrations. We now report that antibiotics inhibit cell wall biosynthesis induce both regulons as monitored using DNA microarrays. Induction of selected ‐dependent genes was confirmed lacZ reporter fusions Northern blot analysis. ability vancomycin to the...
Bacillus subtilis contains seven extracytoplasmic-function sigma factors that activate partially overlapping regulons. We here identify four additional members of the sigma(X) regulon, pbpX (penicillin-binding protein), ywnJ, dlt operon (D-alanylation teichoic acids), and pss ybfM psd (phosphatidylethanolamine biosynthesis). Modification acids by esterification with D-alanine incorporation phosphatidylethanolamine into cell membrane have a common consequence: in both cases positively charged...
ABSTRACT We demonstrate that the Bacillus subtilis fosB(yndN) gene encodes a fosfomycin resistance protein. Expression of fosB requires ς W , and both sigW mutants are sensitive. FosB is metallothiol transferase related to FosA class Mn 2+ -dependent glutathione transferases but with preference for Mg l -cysteine as cofactors.
Streptococcus suis serotype 2 (S. 2, SS2) has evolved into a highly infectious entity, which caused the two recent large-scale outbreaks of human SS2 epidemic in China, and is characterized by toxic shock-like syndrome. However, molecular pathogenesis this new emerging pathogen still poorly understood.89K newly predicted pathogenicity island (PAI) specific to Chinese strains isolated from these outbreaks. Further bioinformatics analysis revealed unique two-component signal transduction...
The Bacillus subtilis sigW gene encodes an extracytoplasmic function (ECF) σ factor that is expressed in early stationary phase from a ‐dependent autoregulatory promoter, P W . Using consensus‐based search procedure, we have identified 15 operons preceded by promoters similar sequence to At least 14 of these are dependent on both vivo and vitro as judged lacZ reporter fusions, run‐off transcription assays nucleotide resolution start site mapping. We conclude controls regulon more than 30...
Bacitracin resistance is normally conferred by either of two major mechanisms, the BcrABC transporter, which pumps out bacitracin, or BacA, an undecaprenol kinase that provides C(55)-isoprenyl phosphate de novo synthesis. We demonstrate Bacillus subtilis bcrC (ywoA) gene, encoding a putative bacitracin transport permease, important determinant. A mutant strain had eightfold-higher sensitivity to bacitracin. Expression initiated from single promoter site could be recognized extracytoplasmic...
NeuB, a sialic acid synthase catalyzes the last committed step of de novo biosynthetic pathway acid, major element bacterial surface structure. Here we report functional NeuB homologue Streptococcus suis, zoonotic agent and systematically address its molecular immunological role in virulence. Disruption neuB led to thinner capsules more susceptibility pH cps2B inactivation resulted complete absence capsular polysaccharides. These two mutants both exhibited increased adhesion invasion Hep-2...
Listeria monocytogenes is an intracellular bacterial pathogen whose virulence depends on the regulated expression of numerous secreted factors. As for other gram-positive bacteria, many proteins by L. are translocated across membrane in unfolded state to compartment existing between and cell wall. This presents a challenging environment protein folding due its high density negative charge, concentrations cations, low pH. We recently identified PrsA2 as gene product required virulence. was...
The Rgg-like regulators, a family of transcription factors commonly found in many Gram-positive bacteria, play multiple roles, especially the control pathogen virulence. Here, we report an rgg homologue from Chinese isolate, 05ZYH33, Streptococcus suis serotype 2 (SS2). Deletion gene SS2 increased its adhesion to Hep-2 cells and hemolytic activity vitro. Significantly, inactivation attenuated virulence experimental piglet infection model. Using DNA microarrays quantitative reverse...
A colloidal gold immunochromatographic assay (ICA) based on a dual-antibody sandwich method was developed for the rapid and convenient detection of Orientia tsutsugamushi (O. tsutsugamushi) antigens in early stages infection. Monoclonal antibodies designed as 5B3 targeting conserved region 56 kDa outer membrane protein various strains O. were generated through cell fusion screening techniques combined with previously prepared polyclonal to establish ICA. Colloidal antibody-colloidal...
Non-heading Chinese cabbage (NHCC) is an important vegetable, and its leaves are harvested for consumption. Thus, the initiation progression of leaf senescence in NHCC directly impact yield quality. In multiple plant species, NAC transcription factors known to act as critical regulators senescence. However, NHCC, contributing regulation remain be identified, mechanisms underlying dark-induced unclear. To explore molecular we stored away from light subsequently examined transcriptional...
In this study, we developed a new mariner-based transposition system for Listeria monocytogenes. The has high rate of and low plasmid retention, is very random, making it an ideal tool high-throughput transposon mutagenesis in L.
Botanicals are rich in bioactive compounds, and some offer numerous beneficial effects to animal human health when consumed. It is well known that phytochemicals cranberries have anti-oxidative antimicrobial activities. Recently, an increasing body of evidence has demonstrated cranberry may potential benefits promote healthy aging. Here, we use Caenorhabditis elegans as a model show water-soluble extract standardized 4.0% proanthocyanidins (WCESP), major component cranberries, can enhance...
ABSTRACT An epidemic of human H7N9 influenza virus infection recently emerged in China whose clinical features include high mortality and which has also resulted serious economic loss. The novel reassortant avian-origin A (H7N9) was the causative agent this raised possibility triggering a large-scale pandemic worldwide. It seemed likely that fast molecular detection assays specific for would be great demand. Here, we report one-step reverse transcription–loop-mediated isothermal...
A growing body of evidence suggests that nutraceuticals with prolongevity properties may delay the onset Alzheimer's disease (AD). We recently demonstrated a proanthocyanidins-standardized cranberry extract has prolong life span and promote innate immunity in Caenorhabditis elegans In this article, we report supplementation delayed Aβ toxicity-triggered paralysis C AD model. Genetic analyses indicated cranberry-mediated toxicity alleviation required heat shock transcription factor (HSF)-1...