A5017336916- Streptococcal Infections and Treatments
- Neonatal and Maternal Infections
- Antimicrobial Resistance in Staphylococcus
- Toxin Mechanisms and Immunotoxins
- Infective Endocarditis Diagnosis and Management
- Plant Pathogenic Bacteria Studies
- Biochemical and Structural Characterization
- Viral Infections and Vectors
- Infections and bacterial resistance
- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Monoclonal and Polyclonal Antibodies Research
- Blood groups and transfusion
- Herpesvirus Infections and Treatments
- Peptidase Inhibition and Analysis
- vaccines and immunoinformatics approaches
- Vector-Borne Animal Diseases
- Genomics and Phylogenetic Studies
- Vector-borne infectious diseases
- Respiratory viral infections research
- Viral Infections and Immunology Research
- Pneumonia and Respiratory Infections
- Animal Virus Infections Studies
- Otolaryngology and Infectious Diseases
- Mosquito-borne diseases and control
Northeast Agricultural University
2024
Hong Kong University of Science and Technology
2024
University of Hong Kong
2024
Shanghai University of Electric Power
2024
South China Normal University
2024
Nanjing General Hospital of Nanjing Military Command
2008-2017
Nanjing University
2016
Jinling Institute of Technology
2015
In-Q-Tel
2011
Army Medical University
2007-2011
Streptococcus suis serotype 2 (S. 2, SS2) is a major zoonotic pathogen that causes only sporadic cases of meningitis and sepsis in humans. Most if not all Streptococcal toxic shock syndrome (STSS) have been well-documented to date were associated with the non-SS2 group A streptococcus (GAS). However, recent large-scale outbreak SS2 Sichuan Province, China, appeared be caused by more invasive deep-tissue infection STSS, characterized acute high fever, vascular collapse, hypotension, shock,...
Background Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen, causing more than 200 cases of severe human infection worldwide, with the hallmarks meningitis, septicemia, arthritis, etc. Very recently, SS2 has been recognized as etiological agent for streptococcal toxic shock syndrome (STSS), which was originally associated pyogenes (GAS) in Streptococci. However, molecular mechanisms underlying STSS are poorly understood.Methods and FindingsTo elucidate genetic...
Streptococcus suis serotype 2 (SS2) has evolved into a highly infectious entity, posing great threat to public health. Screening for and identification of protective antigens plays an important role in developing therapies against SS2 infections.Multiple strategies were used investigate new surface protein that the potential be antigen. These included molecular cloning, biochemical biophysical analyses, enzymatic assay, immunological approaches (eg, immunoelectron microscopy), experimental...
Streptococcus suis serotype 2 (S. 2, SS2) has evolved into a highly infectious entity, which caused the two recent large-scale outbreaks of human SS2 epidemic in China, and is characterized by toxic shock-like syndrome. However, molecular pathogenesis this new emerging pathogen still poorly understood.89K newly predicted pathogenicity island (PAI) specific to Chinese strains isolated from these outbreaks. Further bioinformatics analysis revealed unique two-component signal transduction...
Pathogenicity islands (PAIs), a distinct type of genomic island (GI), play important roles in the rapid adaptation and increased virulence pathogens. 89K is newly identified PAI epidemic Streptococcus suis isolates that are related to two recent large-scale outbreaks human infection China. However, its mechanism evolution contribution spread S. 2 remain unknown. In this study, potential for mobilization was evaluated, putative transfer investigated. We report can spontaneously excise form an...
Streptococcus suis serotype 2 (S. 2) has evolved into a highly invasive pathogen that was found to be the cause of large-scale outbreaks streptococcus toxic shock syndrome (STSS) in China. However, mechanism action this non–group A streptococcal (GAS) S. suis–caused STSS is still unknown. Previously, we identified unique pathogenicity island (PAI) designated 89K specific STSS-causing epidemic strains 2. In study, further report functional type IV–like secretion system (T4SS-like system)...
Streptococcus suis serotype 2 is an emerging zoonotic pathogen responsible for a wide range of life-threatening diseases in pigs and humans. However, the pathogenesis S. infection not well understood. In this study, we report that orphan response regulator, CovR, globally regulates gene expression negatively controls virulence 05ZYH33, streptococcal toxic shock syndrome (STSS)-causing strain. A covR-defective (DeltacovR) mutant 05ZYH33 displayed dramatic phenotypic changes, such as formation...
The Rgg-like regulators, a family of transcription factors commonly found in many Gram-positive bacteria, play multiple roles, especially the control pathogen virulence. Here, we report an rgg homologue from Chinese isolate, 05ZYH33, Streptococcus suis serotype 2 (SS2). Deletion gene SS2 increased its adhesion to Hep-2 cells and hemolytic activity vitro. Significantly, inactivation attenuated virulence experimental piglet infection model. Using DNA microarrays quantitative reverse...
NeuB, a sialic acid synthase catalyzes the last committed step of de novo biosynthetic pathway acid, major element bacterial surface structure. Here we report functional NeuB homologue Streptococcus suis, zoonotic agent and systematically address its molecular immunological role in virulence. Disruption neuB led to thinner capsules more susceptibility pH cps2B inactivation resulted complete absence capsular polysaccharides. These two mutants both exhibited increased adhesion invasion Hep-2...
Zinc is an essential trace element for all living organisms and plays pivotal roles in various cellular processes. However, excess of zinc extremely deleterious to cells. Bacteria have evolved complex machineries (such as efflux/influx systems) control the concentration at levels appropriate maintenance homeostasis cells adaptation environment. The Zur (zinc uptake regulator) protein one these functional members involved precise homeostasis. Here we identified a zur homologue designated 310...
Streptococcus suis has emerged as a causative agent of human meningitis and streptococcal toxic shock syndrome over the last years. The high pathogenicity S. may be due in part to laterally acquired island (renamed SsPI-1), which can spontaneously excise transfer recipients. Cells harboring excised SsPI-1 potentially lose this if cell division occurs prior its reintegration; however, attempts cure from host cells have been unsuccessful. Here, we report that an SsPI-1-borne Epsilon/Zeta...
First International Workshop on Streptococcus suis, Beijing, China, 12–13 August 2013 The first international workshop which is an important swine pathogen and emerging zoonotic agent, took place in jointly organized by the Faculty of Veterinary Medicine, University Montreal, Canada National Institute for Communicable Disease Control Prevention, China CDC. aim meeting was to gather together, time, more than 80 researchers working S. from countries including Canada, Japan, Netherlands,...
Streptococcus suis serotype 2 (S. 2) is an important zoonotic pathogen. It causes heavy economic losses in the pig-farming industry and can be associated with severe infections humans, e.g. streptococcal toxic shock syndrome. Understanding its pathogenesis critical for prevention control of diseases caused by S. 2. In this study, we show that deletion a two-component system (TCS), 05SSU1660/1661 (orthologues Ihk/Irr TCS pyogenes), strain 05ZYH33 results notable attenuation virulence, as...
Streptococcal histidine triad protein was identified recently as a cell surface-associated family. Five members of this family (PhtA, PhtB, PhtD, PhtE and HtpA), derived from Streptococcus pneumoniae pyogenes, have been shown antigens that confer protection to the host on infection. In report, gene sequence highly homologous htpA phtD (designated htpS, suis) S. suis 2 Chinese strain 05ZYH33. Our data revealed htpS is extremely conserved in widely distributed 83% (29/35) 35 serotypes. It also...
Streptococcal histidine triad proteins HTPs are widely distributed within the Streptococcus genus. Based on phylogenetic relationship and domain composition, classified into type I II subfamilies. Previous studies revealed that several pathogenic streptococci contain more than one htp gene. We found highly virulent strain of suis 2 (S. 2), 05ZYH33 encodes 3 HTPs, designated HtpsA (previously described as HtpS), HtpsB, HtpsC. Among them, HtpsC is only member contains leucine-rich repeat (LRR)...
First International Workshop on Streptococcus suis, Beijing, China, 12–13 August 2013 This second and final chapter of the report suis follows from Part 1, published in April 2014, volume 9, issue 4 Future Microbiology. S. is a swine pathogen zoonotic agent afflicting people close contact with infected pigs or pork meat. Although sporadic cases human infections had been reported worldwide, deadly outbreaks emerged Asia. The severity disease underscores lack knowledge virulence evolution this...
Abstract Streptococcus suis serotype 2 is an emerging zoonotic pathogen that triggered two outbreaks of streptococcal toxic shock syndrome (STSS) in China. Our previous research demonstrated a type IV secretion system (T4SS) harbored the 89K pathogenicity island contributes to S. 2. In present study, shotgun proteomics approach was employed identify effectors secreted by T4SS and surface-associated subtilisin-like protease-1 (SspA-1) identified as potential virulence effector. Western blot...
Surface antigen one (Sao) is a newly identified protein from the major zoonotic pathogen, Streptococcus suis. In search of functional proteins related to pathogenesis Chinese S. suis 2 (SS2), unexpectedly, variant Sao was obtained. To test its prevalence in suis, PCR assay adopted address coding genes systematically. It found that there are three allelic variants sao gene, namely sao-S, sao-M, and sao-L based on different lengths (∼1.5, ∼1.7, ∼2.0 kb, respectively). These differences were...
Abstract Streptococcus suis , an emerging infectious pathogen, is the cause of two large-scale outbreaks human streptococcal toxic shock syndrome in China and has attracted much attention from scientific community. The genetic basis its pathogenesis remains enigmatic no effective prevention measures have been established. To better understand virulence differentiation S. develop a promising vaccine, we isolated sequenced native avirulent strain (05HAS68). Animal experiments revealed that...
Emergence of fatal Streptococcus suis serotype 2 (S. 2, SS2) in China, 2005, has greatly challenged the public health, raising serious concerns about its global circulation. Whole genome sequencing representative strains identified a potential pathogenicity island (PAI), named 89K. Here, we reported recurrence human S. meningitis cases (two Shenzhen City and one Sichuan Province) 2007. Multiple lines evidence supported that these are caused by highly pathogenic SS2 isolates. Pulsed-field gel...