A5053308845- Bacterial Infections and Vaccines
- Barrier Structure and Function Studies
- Cerebrospinal fluid and hydrocephalus
- Immune Response and Inflammation
- Connexins and lens biology
- Streptococcal Infections and Treatments
- interferon and immune responses
- RNA Research and Splicing
- Neuroscience of respiration and sleep
- Biochemical Analysis and Sensing Techniques
- Neonatal and fetal brain pathology
- Ion Channels and Receptors
- RNA and protein synthesis mechanisms
- Olfactory and Sensory Function Studies
- Ion Transport and Channel Regulation
- Neuroscience and Neuropharmacology Research
- Escherichia coli research studies
- Listeria monocytogenes in Food Safety
- Amoebic Infections and Treatments
- Cell death mechanisms and regulation
- Microbial Inactivation Methods
- Neurogenesis and neuroplasticity mechanisms
- Drug Transport and Resistance Mechanisms
- Gut microbiota and health
- Inflammasome and immune disorders
Heidelberg University
2016-2025
University Hospital Heidelberg
2016-2025
University Medical Centre Mannheim
2016-2025
Heidelberg University
2016-2022
Heinrich Heine University Düsseldorf
2003-2010
Boston Children's Hospital
2009
Institute of Molecular Medicine
2009
Düsseldorf University Hospital
2008
Klinik und Poliklinik für Kinder- und Jugendmedizin
2005
Howard Hughes Medical Institute
2003
Different isoforms of a protein complex termed the apoptosis- and splicing-associated (ASAP) were isolated from HeLa cell extract. ASAP complexes are composed polypeptides SAP18 RNPS1 different Acinus protein. While had previously been implicated in apoptosis was recently identified as component spliceosome, has described general activator RNA processing. Addition to vitro splicing reactions inhibits processing mediated by ASF/SF2, SC35, or RNPS1. Additionally, microinjection into mammalian...
Acute bacterial meningitis is a life-threatening disease in humans. Discussed as entry sites for pathogens into the brain are blood-brain and blood-cerebrospinal fluid barrier (BCSFB). Although human microvascular endothelial cells (HBMEC) constitute well established vitro model barrier, until now no reliable system presenting BCSFB has been developed. Here, we describe first time functional based on choroid plexus papilloma (HIBCPP), which display typical hallmarks of expression junctional...
A critical point during the course of central nervous system infection is influx leukocytes from blood into brain across blood-brain barrier (BBB) and blood-cerebrospinal fluid (BCSFB). However, experimental in vitro models to investigate leukocyte transmigration cultured choroid plexus epithelial cells have been lacking so far.We developed a porcine human "inverted" culture insert that enables specifically physiologically relevant basolateral side. The use primary (PCPEC) papilloma...
Previous experimental studies in a standard Transwell culture system have shown Streptococcus suis ability to compromise barrier function of porcine choroid plexus epithelial cells (PCPEC). The development an 'inverted' filter PCPEC enables us now for the first time investigate bacterial invasion and translocation from physiologically relevant basolateral (blood) apical (cerebrospinal fluid) side. Most importantly, we observed specific S. across exclusively During this process, viability...
Abstract Background A critical point during the course of bacterial meningitis is excessive influx polymorphnuclear neutrophils (PMNs) from blood into brain. Both paracellular and transcellular routes leukocyte transmigration through blood-brain barrier have been described in CNS diseases so far. Thus, we investigated mechanism PMN blood-CSF under inflammatory conditions. Methods In an "inverted" Transwell culture model barrier, zoonotic agent Streptococcus suis ( S. ) was used to stimulate...
The human choroid plexus (CP) is the location of blood-cerebrospinal fluid (CSF) barrier (BCSFB). Whereas epithelial cells CP mainly contribute to formation BCSFB, vessels are built by fenestrated endothelial cells. Still, endothelium can function. By ectopic expression telomerase reverse transcriptase (hTERT) in primary (HCPEnCs), we recently generated and characterized immortalized HCPEnCs (iHCPEnCs). Here, compared sixth passage (HCPEnCs p6) with a lower (p20) higher (p50) iHCPEnCs...
Abstract Background Bacterial invasion through the blood-cerebrospinal fluid barrier (BCSFB) during bacterial meningitis causes secretion of proinflammatory cytokines/chemokines followed by recruitment leukocytes into CNS. In this study, we analyzed cellular and molecular mechanisms polymorphonuclear neutrophil (PMN) monocyte transepithelial transmigration (TM) across BCSFB after infection. Methods Using an inverted transwell filter system human choroid plexus papilloma cells (HIBCPP),...
The epithelial cells of the choroid plexus (CP), located in ventricular system brain, form blood-cerebrospinal fluid barrier (BCSFB). BCSFB functions separating cerebrospinal (CSF) from blood and restricting molecular exchange to a minimum extent. An vitro model is based on derived human papilloma (HIBCPP). HIBCPP display typical including formation tight junctions (TJs), development transepithelial electrical resistance (TEER), as well minor permeabilities for macromolecules. There are...
Abstract The rate of SARS-CoV-2 infections in children remains unclear due to many asymptomatic cases. We present a study cross-sectional seroprevalence surveys anti-SARS-CoV-2 IgG 10,358 recruited paediatric hospitals across Germany from June 2020 May 2021. Seropositivity increased 2.0% (95% CI 1.6, 2.5) 10.8% 8.7, 12.9) March 2021 with little change up Rates by migrant background (2.8%, 4.4% and 7.8% for no, one two parents born outside Germany). Children under three were initially 3.6...
RNPS1, Acinus, and SAP18 form the apoptosis- splicing-associated protein (ASAP) complex, which is also part of exon junction complex. Whereas RNPS1 was originally identified as a general activator mRNA processing, all three proteins have been found within functional spliceosomes. Both Acinus contain typical motifs splicing regulatory including arginine/serine-rich domains. Due to absence such structural features, however, function in regulation completely unknown. Here we investigated...