A5101458666- Streptococcal Infections and Treatments
- Neonatal and Maternal Infections
- Antimicrobial Resistance in Staphylococcus
- Photonic and Optical Devices
- Toxin Mechanisms and Immunotoxins
- Photonic Crystals and Applications
- Plant Pathogenic Bacteria Studies
- Bacteriophages and microbial interactions
- Infective Endocarditis Diagnosis and Management
- Biosensors and Analytical Detection
- Respiratory viral infections research
- Monoclonal and Polyclonal Antibodies Research
- Advanced Fiber Laser Technologies
- Silicon Nanostructures and Photoluminescence
- Immune Response and Inflammation
- Spectroscopy and Quantum Chemical Studies
- Mosquito-borne diseases and control
- Inflammasome and immune disorders
- Viral gastroenteritis research and epidemiology
- Advanced biosensing and bioanalysis techniques
- Photonic Crystal and Fiber Optics
- Viral Infections and Vectors
- Cancer Research and Treatments
- Optical Network Technologies
- Genomics and Phylogenetic Studies
Academy of Military Medical Sciences
2016-2025
Anhui Medical University
2014-2025
Institute of Microbiology
2015-2024
University of Science and Technology Liaoning
2023-2024
Jiangsu University
2024
Czech Academy of Sciences, Institute of Microbiology
1998-2024
Qinghai University
2024
Guangxi Academy of Sciences
2012-2023
Institute of Microbiology
2016-2023
Zhongkai University of Agriculture and Engineering
2023
Background Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen, causing more than 200 cases of severe human infection worldwide, with the hallmarks meningitis, septicemia, arthritis, etc. Very recently, SS2 has been recognized as etiological agent for streptococcal toxic shock syndrome (STSS), which was originally associated pyogenes (GAS) in Streptococci. However, molecular mechanisms underlying STSS are poorly understood.Methods and FindingsTo elucidate genetic...
An ultracompact silicon electro-optic modulator was experimentally demonstrated based on photonic crystal (PhC) waveguides for the first time to our knowledge. Modulation operation by carrier injection into an 80μm-long PhC waveguide of a Mach-Zehnder interferometer (MZI) structure. The π phase shift driving current, Iπ, across active region is as low 0.15mA, which equivalent Vπ 7.5mV when 50Ω impedance-matched structure applied. modulation depth 92% operating at 1567nm.
ABSTRACT Streptococcus suis serotype 2 is a Gram-positive bacterium that causes sepsis and meningitis in piglets humans. The mechanisms of S. invasive disease are not well understood. surface proteins pathogens usually play important roles infection bacterium-host interactions. Here, we identified novel protein contributed significantly to the virulence piglet model. This showed little similarity other reported exhibited strong binding activity human factor H (hFH). It was designated Fhb...
Urinary tract infections (UTIs) are among the most common acquired bacterial in humans. The current gold standard method for identification of uropathogens clinical laboratories is cultivation. However, culture-based assays have substantial drawbacks, including long turnaround time and limited culturability many potential pathogens. Nanopore sequencing technology can overcome these limitations detect pathogens while also providing reliable predictions drug susceptibility samples. Here, we...
Streptococcus suis serotype 2 (S. 2) is a highly invasive pathogen in pigs and humans that can cause severe systemic infection. Sepsis meningitis are the most common clinical manifestations of S. However, mechanisms surviving human blood remains unclear, so to identify novel virulence factors evasion polymorphonuclear leukocyte (PMN)-mediated innate immunity play important roles developing therapies against Here, we found escape phagocytic clearance by adenosine synthesis blood. Through...
Lipid A is an essential basal component of lipopolysaccharide most Gram-negative bacteria. Inhibitors targeting LpxC, a conserved enzyme in lipid biosynthesis, are antibiotic candidates against pathogens. Here we report the characterization role Coxiella burnetii growth axenic media, monkey kidney cells (BGMK and Vero) macrophage-like THP-1 by using potent LpxC inhibitor -LPC-011. We first determined susceptibility C. to LPC-011 surrogate E. coli model. In coli, minimum inhibitory...
Muramidase-released protein (MRP) is as an important virulence marker of Streptococcus suis (S. suis) serotype 2. Our previous works have shown that MRP can bind human fibrinogen (hFg); however, the function this interaction in S. meningitis not known. In study, we found deletion mrp significantly impairs hFg-mediated adherence and traversal ability across cerebral microvascular endothelial cells (hCMEC/D3). Measurement permeability to Lucifer yellow vitro Evans blue extravasation vivo show...
The early identification of pathogens and their antibiotic resistance are essential for the management treatment patients affected by ventilator-associated pneumonia (VAP). However, microbiological culture may be time-consuming has a limited culturability many potential pathogens. In this study, we developed rapid nanopore-based metagenomic next-generation sequencing (mNGS) diagnostic assay detection VAP antimicrobial genes (ARGs).Endotracheal aspirate (ETA) samples from 63 with suspected...
Tunable optical true time-delay modules based on highly dispersive photonic crystal fibers (PCFs) are demonstrated to provide continuous radio-frequency squint-free beam scanning for an X-band (8-12 GHz) phased array antenna system. The dispersion of the fabricated PCF is as high -600 ps/nm /spl middot/ km at 1550 nm. time delay continuously tunable from -31 31 ps between adjacent lines by tuning laser wavelength 1528 1560 far field radiation patterns a 1/spl times/4 subarray were measured...
Background Whole-cell matrix–assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) has been successfully applied for bacterial identification and typing of many pathogens. The fast reliable qualities MALDI-TOF MS make it suitable clinical diagnostics. the cluster analysis Streptococcus pyogenes, however, not reported. goal our study was to evaluate this approach rapid S. pyogenes. Methods 65 pyogenes isolates were obtained from hospital. samples prepared...
Streptococcus suis serotype 2 (SS2) induced sepsis and meningitis are often accompanied by bacteremia. However, the mechanism whereby it helps S. to evade PMN-mediated phagocytosis remain unclear. Because of central roles bacteria-human fibrinogen (hFg) interaction in innate immunity, here, a proteomics based Far-western blotting (PBFWB) was developed identify fibrinogen-binding surface proteins (SsFBPs) on large-scale. And then thirteen potential SsFBPs were identified PBFWB we selected...
Abstract Background Early availability of pathogen identification in urinary tract infections (UTIs) has critical importance disease management. Metagenomic next-generation sequencing (mNGS) the potential to transform how acute and serious are diagnosed by offering unbiased culture-free detection. However, clinical experience with application mNGS test is relatively limited. Methods We therefore established a MinION-based pathogens diagnostic platform evaluated its for implementation UTIs...
As one of the key diagnostic methods for detecting biomarkers and antigen-antibody interactions, luminescent oxygen channel immunoassay (LOCI) has been widely applied in bioanalysis other fields. In context LOCI, performance prepared donor polystyrene (PS) microspheres significantly impacts detection signal values. this study, an attempt was made to synthesize PS via one-step polymerization styrene with amphiphilic monomer (PEOOH), followed by swelling silicon phthalocyanine photosensitizer...
ABSTRACT Recent metagenomic studies have identified a vast number of viruses. However, the systematic assessment true genetic diversity whole virus community on our planet remains to be investigated. Here, we explored genome and protein space viruses by simulating process discovery in viral studies. Among multiple functions, power function was found best fit increasing trends was, therefore, used predict The estimate suggests that there are at least 8.23e+08 operational taxonomic units...
The isolation and characterization of novel probiotics from dairy products, fermented foods, the gut have gained significant attention. In particular, Streptococcus salivarius shows promise for use in oral probiotic preparations. this study, we isolated two strains S. salivarius—S.82.15 S.82.20—from cavity a healthy individual. These exhibited distinct antimicrobial profiles. We thoroughly assessed morphology growth patterns both confirmed auto-aggregation hemolytic activity. Through...
Optical second-harmonic generation (SHG) is used as a noninvasive probe of the interfaces Si nanocrystals (NCs) embedded uniformly in an SiO2 matrix. Measurements generated SH mode verify that polarization has nonlocal dipole form proportional to (E x Delta inverted) E depends on inhomogeneities incident field E, proposed recent models based locally noncentrosymmetric dipolar response averaged over spherical NC interfaces. A two-beam SHG geometry found enhance this greatly compared...
Streptococcus suis, a zoonotic pathogen, caused serious outbreaks in humans with high mortality rates the past decade. To develop safer and more effective vaccines, particularly for human protection, cell wall extracellular proteins of S. suis serotype 2 were analyzed by an immunoproteomic approach this study. Thirty-two immunogenicity identified 22 them newly identified. Further analyses 9 selected revealed that (1) these expressed all tested virulent isolates, (2) antisera against 6...
First International Workshop on Streptococcus suis, Beijing, China, 12–13 August 2013 The first international workshop which is an important swine pathogen and emerging zoonotic agent, took place in jointly organized by the Faculty of Veterinary Medicine, University Montreal, Canada National Institute for Communicable Disease Control Prevention, China CDC. aim meeting was to gather together, time, more than 80 researchers working S. from countries including Canada, Japan, Netherlands,...
Metagenomic next-generation sequencing (mNGS) is a novel useful strategy that increasingly used for pathogens detection in clinic. Some emerging mNGS technologies with long-read ability are to decrease time and increase diagnosed accuracy, which of great significance rapid pathogen diagnosis. Reliable DNA extraction considered critical the success sequencing; hence, there thus an urgent need gentle method get unbiased more integrate from all kinds pathogens. In this study, we systematically...