A5018309024- Bacteriophages and microbial interactions
- Bacillus and Francisella bacterial research
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Genomics and Phylogenetic Studies
- Brucella: diagnosis, epidemiology, treatment
- CRISPR and Genetic Engineering
- vaccines and immunoinformatics approaches
- Advanced Graph Neural Networks
- Vibrio bacteria research studies
- SARS-CoV-2 and COVID-19 Research
- Bacterial biofilms and quorum sensing
- Viral gastroenteritis research and epidemiology
- Yersinia bacterium, plague, ectoparasites research
- Complex Network Analysis Techniques
- Identification and Quantification in Food
- Cancer Research and Treatments
- Bacterial Infections and Vaccines
- Salmonella and Campylobacter epidemiology
- Enzyme Structure and Function
- RNA Interference and Gene Delivery
- Insect Resistance and Genetics
Shanghai Ocean University
2015-2025
Academy of Military Medical Sciences
2006-2025
Shenzhen Technology University
2023-2024
Beijing Fengtai Hospital
2024
Czech Academy of Sciences, Institute of Biotechnology
2003-2023
Tsinghua University
2022
Peking University
2018-2020
Henan Academy of Agricultural Sciences
2017-2020
Quanta Computer (China)
2020
Institute of Plant Protection
2017-2020
Abstract Recent years have seen enormous advances in nanovaccines for both prophylactic and therapeutic applications, but most of these technologies employ chemical or hybrid semi‐biosynthetic production methods. Thus, has to date failed exploit biology‐only processes like complex sequential post‐translational biochemical modifications scalability, limiting the realization initial promise offering major performance advantages improved outcomes over conventional vaccines. A Nano‐B5 platform...
The atypical pneumonia (COVID-19) caused by SARS-CoV-2 is a serious threat to global public health. However, early detection and effective prediction of patients with mild severe symptoms remain challenging. proteomic profiling urine samples from healthy individuals, COVID-19 positive comorbidities can be clearly differentiated. Multiple pathways have been compromised after the infection, including dysregulation complement activation, platelet degranulation, lipoprotein metabolic process...
Conjugate vaccines are known to be one of the most effective and safest types against bacterial pathogens. Previously, vaccine biosynthesis has been performed by using N-linked glycosylation systems. However, structural specificity these systems for sugar substrates hindered their application. Here, we report a novel protein system (O-linked via Neisseria meningitidis) that can transfer virtually any glycan produce conjugate vaccine. We successfully established this in Shigella spp.,...
A comprehensive proteomic study was carried out to identify and characterize proteins expressed by Bifidobacterium longum NCC2705. total of 708 spots representing 369 protein entries were identified MALDI-TOF-MS and/or ESI-MS/MS. Isoelectric point values estimated gel electrophoresis matched closely with their predicted ones, although some discrepancies exist suggesting that post-translational modifications might be common in B. longum. The represent 21.4% the 1727 ORFs genome correspond 30%...
Abstract Klebsiella pneumoniae has emerged as a severe opportunistic pathogen with multiple drug resistances. Finding effective vaccines against this is urgent. Although O‐polysaccharides (OPS) of K. are suitable antigens for the preparation given their low levels diversity, immunogenicity (especially serotype O2) limit application. In study, general Escherichia coli host system developed to produce nanoscale conjugate vaccine using Nano‐B5 self‐assembly platform. The experimental data...
Abstract Background Engineered strains of Escherichia coli have been used to produce bioconjugate vaccines using Protein Glycan Coupling Technology (PGCT). Nanovaccines also entered the vaccine development arena with advances in nanotechnology and significantly developed, but chassis cells for conjugate nanovaccines not reported. Results To facilitate nanovaccine preparation, a generic recombinant protein (SpyCather4573) was as acceptor O-linked glycosyltransferase PglL, glycol-engineered...
Background: Extra-intestinal pathogenic Escherichia coli (ExPEC) represents a major global public health challenge due to its ability cause diverse clinical infections, including urinary tract bacteremia, neonatal meningitis, and sepsis. The growing prevalence of multidrug-resistant (MDR) ExPEC strains, which rapidly erode antibiotic efficacy, underscores vaccine development as critical priority. Bioconjugate vaccines have emerged promising approach mitigate ExPEC-associated infections....
Streptococcus suis serotype 2 (SS2) induced sepsis and meningitis are often accompanied by bacteremia. However, the mechanism whereby it helps S. to evade PMN-mediated phagocytosis remain unclear. Because of central roles bacteria-human fibrinogen (hFg) interaction in innate immunity, here, a proteomics based Far-western blotting (PBFWB) was developed identify fibrinogen-binding surface proteins (SsFBPs) on large-scale. And then thirteen potential SsFBPs were identified PBFWB we selected...
Enteric fever, mainly caused by Salmonella enterica serovar Paratyphi A, remains a common and serious infectious disease worldwide. As yet, there are no licensed vaccines against S. A. Biosynthesis of conjugate has become promising approach bacterial infection. However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize specialized O-polysaccharide structure Here, we describe an O-linked approach, only currently available system suitable for A vaccine....
Streptococcus suis, a zoonotic pathogen, caused serious outbreaks in humans with high mortality rates the past decade. To develop safer and more effective vaccines, particularly for human protection, cell wall extracellular proteins of S. suis serotype 2 were analyzed by an immunoproteomic approach this study. Thirty-two immunogenicity identified 22 them newly identified. Further analyses 9 selected revealed that (1) these expressed all tested virulent isolates, (2) antisera against 6...
The large plasmid pXO1 encoding the anthrax toxin is important for virulence of Bacillus anthracis. It essential to cure from B. anthracis evaluate its role in pathogenesis infection. Because conventional methods curing plasmids (e.g., agents or growth at elevated temperatures) can induce mutations host chromosomal DNA, we developed a specific and reliable method eliminate using incompatibility. Three putative replication origins were inserted into temperature-sensitive generate three...
Genome editing is an effective tool for the functional examination of bacterial genes and live attenuated vaccine construction. Here, we report a method to edit genomic DNA Bacillus anthracis cereus using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas)9 system. Using two prophages in B. as targets, large-fragment deletion mutants were achieved with rates 100% or 20%. In cereus, successfully introduced precise point mutations into plcR,...
As next-generation pathogen detection methods, CRISPR-Cas-based methods can perform single-nucleotide polymorphism (SNP) level with high sensitivity and good specificity. They do not require any particular equipment, which opens up new possibilities for the accurate identification of Bacillus anthracis. In this study, we developed a complete system B. anthracis based on Cas12a. We used two chromosomally located SNP targets plasmid to identify accuracy. The CR5 target is completely new....
Multivalent antigen display on nanoparticles can enhance the immunogenicity of nanovaccines targeting viral moieties, such as receptor binding domain (RBD) SARS-CoV-2. However, particle morphology and size current are significantly different from those Additionally, surface patterns not controllable to enable optimization B cell activation. Herein, we employ an icosahedral DNA origami (ICO) a for RBD nanovaccines, achieving diameter like virus (91 ± 11 nm). The addressability permits facile...
Summary Acinetobacter baumannii poses a serious threat to human health, mainly because of its widespread distribution and severe drug resistance. However, no licensed vaccines exist for this pathogen. In study, we created conjugate vaccine against A. by introducing an O‐linked glycosylation system into the host strain. After demonstrating ability elicit Th1 Th2 immune responses observing good safety in mouse model, strong vitro bactericidal activity prophylactic effects infection were...
With the emergence of multidrug-resistant strains, Acinetobacter baumannii infection is becoming a thorny health problem in hospitals. However, there are no licensed vaccines against A. . trimeric autotransporter (Ata) an important known virulence factor located on outer membrane bacteria. Herein, we carried out series experiments to test immunogenicity short C-terminal extracellular region Ata (Ata α , only containing 39 amino acids) murine model. The peptide was fused with cholera toxin B...
Abstract Shigella flexneri is a Gram‐negative facultatively intracellular pathogen responsible for bacillary dysentery in humans. In this study, extracellular proteins from the culture medium and whole cell cellular extracts of S. 2a strain 2457T were examined by two‐dimensional (2‐D) gel electrophoresis using immobilized pH gradient (IPG) technology. Proteins identified matrix‐assisted laser desorption/ionization‐mass spectrometry (MALDI‐MS) combination with Mascot search program. total,...
Brucellosis is a major zoonotic public health threat worldwide, causing veterinary morbidity and economic losses in endemic regions. However, no efficacious brucellosis vaccine yet available, live attenuated vaccines commonly used animals can cause human infection. N- O-linked glycosylation systems have been successfully developed exploited for the production of successful bioconjugate vaccines. Here, we applied an system to low-pathogenicity bacterium, Yersinia enterocolitica serotype O:9...
Nanoparticles (NPs) have been widely utilized in vaccine design. Although numerous NPs explored, with adjuvant effects on their own rarely reported. We produce a promising self-assembled NP by integrating the pentameric Escherichia coli heat-labile enterotoxin B subunit (LTB) (studied as adjuvant) trimer-forming peptide. This fusion protein can self-assemble into during expression, and polysaccharide antigens (OPS) are then loaded vivo using glycosylation. initially produced two Salmonella...
Nanoparticles (NPs) have been surfacing as a pivotal platform for vaccine development. In our previous work, we developed cholera toxin B subunit (CTB)-based self-assembled nanoparticle (CNP) and produced highly promising bioconjugate nanovaccines by loading bacterial polysaccharide (OPS) in vivo. particular, the Klebsiella pneumoniae O2 serotype showcased potent immune response protection against infection. However, extremely low yields limited its further application. this study, prepared...