A5102981940- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Nanoplatforms for cancer theranostics
- CRISPR and Genetic Engineering
- Dendrimers and Hyperbranched Polymers
- MicroRNA in disease regulation
- Fluid Dynamics and Turbulent Flows
- Gold and Silver Nanoparticles Synthesis and Applications
- Turbomachinery Performance and Optimization
- Cancer Research and Treatment
- Heme Oxygenase-1 and Carbon Monoxide
- Hydrogen embrittlement and corrosion behaviors in metals
- Animal Virus Infections Studies
- Anesthesia and Pain Management
- Combustion and flame dynamics
- Heat Transfer Mechanisms
- Corrosion Behavior and Inhibition
- Pain Mechanisms and Treatments
- Hydraulic Fracturing and Reservoir Analysis
- Inflammatory Biomarkers in Disease Prognosis
- Autism Spectrum Disorder Research
- Pluripotent Stem Cells Research
- Aldose Reductase and Taurine
Chinese Academy of Sciences
2011-2025
The Fourth People's Hospital of Ningxia Hui Autonomous Region
2024-2025
University of Chinese Academy of Sciences
2021-2025
Institute of Zoology
2022-2025
Commercial Aircraft Corporation of China (China)
2015-2024
Institute for Stem Cell Biology and Regenerative Medicine
2024
Northwest University
2024
The University of Texas Southwestern Medical Center
2018-2023
First Affiliated Hospital of Wannan Medical College
2023
Nanjing University of Chinese Medicine
2022
This work demonstrated that ultrasmall gold nanoparticles (AuNPs) smaller than 10 nm display unique advantages over larger in terms of localization to, and penetration of, breast cancer cells, multicellular tumor spheroids, tumors mice. Au@tiopronin have tunable sizes from 2 to 15 with identical surface coatings tiopronin charge were successfully prepared. For monolayer the NPs, more AuNPs found each cell. In addition, accumulation Au NPs ex vivo model was size-dependent: able penetrate...
Abstract CRISPR-Cas9 has emerged as a powerful technology that relies on Cas9/sgRNA ribonucleoprotein complexes (RNPs) to target and edit DNA. However, many therapeutic targets cannot currently be accessed due the lack of carriers can deliver RNPs systemically. Here, we report generalizable methodology allows engineering modified lipid nanoparticles efficiently into cells tissues including muscle, brain, liver, lungs. Intravenous injection facilitated tissue-specific, multiplexed editing six...
Significance Nanotechnology-based drug delivery is expected to bring new hope for cancer treatment by enhancing anticancer efficacy, overcoming resistance, and reducing toxicity. In this respect, we developed an innovative system based on a self-assembling amphiphilic dendrimer, which can generate supramolecular nanomicelles with large void space in their core encapsulate drugs high loading capacity. The resulting drug-encapsulated effectively enhance potency combat resistance promoting...
Nanoparticles offer potential as drug delivery systems for chemotherapeutics based on certain advantages of molecular drugs. In this study, we report that particle size exerts great influence the penetration and retention behavior nanoparticles entering tumors. On comparing gold-coated Au@tiopronin were prepared with identical coating surface properties, found 50 more effective in all vitro, ex vivo, vivo assays conducted using MCF-7 breast cells a model system. Beyond superior cultured cell...
mRNA-mediated protein replacement represents a promising concept for the treatment of liver disorders. Children born with fumarylacetoacetate hydrolase (FAH) mutations suffer from Hepatorenal Tyrosinemia Type 1 (HT-1) resulting in renal dysfunction, failure, neurological impairments, and cancer. Protein therapy using FAH mRNA offers tremendous potential to cure HT-1, but is currently hindered by development effective carriers that can function diseased livers. Structure-guided, rational...
Abstract Approximately 10% of Cystic Fibrosis (CF) patients, particularly those with CF transmembrane conductance regulator ( CFTR ) gene nonsense mutations, lack effective treatments. The potential correction therapy through delivery the CRISPR/Cas system to CF-relevant organs/cells is hindered by efficient genome editor carriers. Herein, we report improved Lung Selective Organ Targeting Lipid Nanoparticles (SORT LNPs) for Cas9 mRNA, sgRNA, and donor ssDNA templates, enabling precise...
Poor penetration of therapeutic drugs into tumors is a major challenge in anticancer therapy, especially solid tumors, leading to reduced efficacy vivo. In the study, we used new tumor-penetrating peptide, CRGDK, conjugate onto surface doxorubicin encapsulated nanoscale micelles. The CRGDK peptide triggered specific binding neuropilin-1, enhanced cellular uptake and cytotoxicity vitro highly accumulation
CRISPR/Cas9-based genome editing has quickly emerged as a powerful breakthrough technology for use in diverse settings across biomedical research and therapeutic development. Recent efforts toward understanding gene modification methods vitro have led to substantial improvements ex vivo efficiency. Because disease targets genomic correction are often localized specific organs, realization of the full potential medicines will require delivery CRISPR/Cas9 systems targeting tissues cells...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is a deadly cancer with no efficacious treatment. The application of nanomedicine expected to bring new hope PDAC In this study, we report novel supramolecular dendrimeric nanosystem carrying the anticancer drug doxorubicin, which demonstrated potent activity, markedly overcoming heterogeneity response and resistance primary cultured tumor cells derived from patients. This dendrimer nanodrug was constructed fluorinated amphiphilic dendrimer,...
Abstract Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR‐associated (Cas) protein gene editing is poised to transform the treatment of genetic diseases. However, limited progress has been made toward precise DNA via homology‐directed repair (HDR) that requires careful orchestration complex steps. Herein, dendrimer‐based lipid nanoparticles (dLNPs) are engineered co‐encapsulate and deliver multiple components for in vivo HDR correction. BFP/GFP switchable HEK293 cells...
Synergistic effects of anticancer drug and siRNA have displayed superior advantages for cancer therapy. Herein, we deeply analyzed the feasibility that whether doxorubicin (DOX) could be co-delivered by mPEG-PCL-graft-PDMAEMA (PECD) micelles, which mediated excellent DNA/siRNA delivery in vitro vivo reported our previous work. DOX-loaded NPs (PECD-D) were developed nanoprecipitation technology exhibited high loading content (DLC, 9.5%). In cytotoxicity study MDA-MB-231 cells, PECD-D treated...
Multidrug resistance (MDR) of cancer is a challenge to effective chemotherapeutic interventions. The stimulus-responsive drug delivery system (DDS) based on nanotechnology provides promising approach overcome MDR. Through the development doxorubicin zinc oxide nanomaterials, we have demonstrated that MDR in breast cell line can be significantly circumvented by combination efficient cellular uptake and pH-triggered rapid release due degradation nanocarriers acidic environment. Doxorubicin...