A5011710088- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Advanced Polymer Synthesis and Characterization
- Genetic Mapping and Diversity in Plants and Animals
- Electrospun Nanofibers in Biomedical Applications
- Hydrogels: synthesis, properties, applications
- Nanoparticle-Based Drug Delivery
- Photopolymerization techniques and applications
- Virus-based gene therapy research
- Nanoplatforms for cancer theranostics
- Wound Healing and Treatments
- Advanced Drug Delivery Systems
- Nonlinear Optical Materials Studies
- Luminescence and Fluorescent Materials
- Genetics and Plant Breeding
- Crop Yield and Soil Fertility
- Gas Sensing Nanomaterials and Sensors
- Hemostasis and retained surgical items
- Wheat and Barley Genetics and Pathology
- Photochromic and Fluorescence Chemistry
- Silicone and Siloxane Chemistry
- Adsorption and biosorption for pollutant removal
- Dendrimers and Hyperbranched Polymers
- Luminescence Properties of Advanced Materials
- Surgical Sutures and Adhesives
Tianjin University
2016-2025
Beijing Academy of Agricultural and Forestry Sciences
2009-2023
Tianjin University of Science and Technology
2021-2022
Bengbu Medical College
2021
Zhejiang University
2020
Zhejiang Hospital
2020
Institute of Soil Science
2016-2020
Chinese Academy of Sciences
2016-2020
University of Chinese Academy of Sciences
2020
Collaborative Innovation Center of Chemical Science and Engineering Tianjin
2015-2016
Charge-reversal functional gold nanoparticles first prepared by layer-by-layer technique were employed to deliver small interfering RNA (siRNA) and plasmid DNA into cancer cells. Polyacrylamide gel electrophoresis measurements of siRNA confirmed the occurrence charge-reversal property nanoparticles. The expression efficiency enhanced green fluorescent protein (EGFP) was improved adjuvant transfection with nanoparticles, which also had much lower toxicity cell proliferation. Lamin A/C, an...
pH-dependent temperature- sensitive poly(ε-caprolactone)-graft-poly(2-(dimethylamino) ethyl methacrylate) (PCL-g-PDMAEMA), a kind of degradable, amphiphilic, cationic copolymer, was synthesized. PCL-g-PDMAEMA self-assembled into core-shell nanoparticles with an ultralow critical association concentration at about 8.1 × 10−4 g L−1. It found that were able to simultaneously entrap hydrophobic paclitaxel and load DNA. Hydrophobic drug paclitaxel, loaded by NPs, could be released faster in...
Salt stress significantly restricts plant growth and production. Maize is an important food economic crop but also a salt sensitive crop. Identification of the genetic architecture controlling tolerance facilitates breeders to select tolerant lines. However, critical quantitative trait loci (QTLs) responsible for field-grown maize plants are still unknown.
Efficient small interfering RNA (siRNA) delivery in the presence of serum is crucial importance for effective gene therapy. Fluorinated vectors are considered to be attractive candidates siRNA-mediated therapy because their efficacy serum-containing media. However, mechanisms driving superior transfection behavior fluorinated still not well-understood, and comprehensive investigations warranted. Herein, we fabricated a library perfluorooctanoyl fluoride-fluorinated (PFF-fluorinated)...
We studied methoxy poly(ethylene glycol)-b-poly(ε-caprolactone)-b-poly(2-dimethylaminoethyl methacrylate) (mPEG-b-PCL-b-PDMAEMA) nanoparticles as the codelivery vector of hydrophobic drug and pDNA by employing dynamic light scattering (DLS), ζ potential, transmission electron microscopy (TEM), gel retardation assay, confocal microscopy, subsequently its in vitro cytotoxicity transfection efficiency were tested. mPEG-b-PCL-b-PDMAEMA (NPs) with or without paclitaxel are both able to complex...
The microtechnology of controlling stimuli-responsive biomaterials at micrometer scale is crucial for biomedical applications. Here, we report bovine serum albumin (BSA)-based three-dimensional (3D) microstructures with tunable surface morphology and pH-responsive properties via two-photon polymerization microfabrication technology. laser processing parameters, including power, scanning speed, layer distance, are optimized the fabrication well-defined 3D BSA microstructures. a wide range pH...
The control of the thermal gelation behavior amphiphilic copolymers based on PEGylated hydrophobic polymers is important for applications in drug administration and controlled release. This paper studied a new method to by structure modification segments. A kind triblock copolymer PEG modified poly(ε-caprolactone) (PCL) with cyclic ether pendant groups, i.e. poly(ε-caprolactone-co-1,4,8-trioxa[4.6]spiro-9-undecanone)-poly(ethylene...
S-type cytoplasmic male sterility (CMS-S) is the largest group among three major types of CMS in maize. CMS-S exhibits fertility instability as a partial restoration specific nuclear genetic background, which impedes its commercial application hybrid breeding programs. The phenomenon controlled by several minor quantitative trait locus (QTLs), but not restorer (Rf3). However, gene mapping these QTLs and molecular mechanism modifications are still unclear. Using completely sterile partially...
High-efficiency gene transfer and suitably low cytotoxicity are the main goals of transfection systems based on nonviral vectors. In addition, it is desirable to track process in order observe explain mechanism. Herein, inspired by viral structures that optimized for delivery, we designed a small-molecule vector (TR4) with aggregation-induced emission properties capping peptide containing four arginine residues tetraphenylethene (TPE) lipophilic tail. This novel can self-assemble plasmid DNA...
Cull cow meat myofibrillar protein (CCMP) filled with carboxymethylated cellulose nanofibrils (cCNF) was initially characterized to study the effect of cCNF on gelation properties CCMP. Before heating, increased degree substitution (DS) cCNF, ζ-potential and hydrophobicity CCMP-cCNF solution at same filler mass fractions (ϕm). During process shortened after adding especially samples high DS. After lower DS led liquid loss gels, amplified ϕm cCNF. Similarly, network structure low more compact...