A5068639635- Alkaline Phosphatase Research Studies
- Carbon Nanotubes in Composites
- Graphene and Nanomaterials Applications
- Extracellular vesicles in disease
- Nanoplatforms for cancer theranostics
- Nanoparticles: synthesis and applications
- RNA Interference and Gene Delivery
- Bone Tissue Engineering Materials
- Bone and Dental Protein Studies
- Advanced biosensing and bioanalysis techniques
- S100 Proteins and Annexins
- Galectins and Cancer Biology
- Parathyroid Disorders and Treatments
- Heterotopic Ossification and Related Conditions
- Nanoparticle-Based Drug Delivery
- Bone and Joint Diseases
- Nanotechnology research and applications
- Protein Tyrosine Phosphatases
- Connective tissue disorders research
- Molecular Junctions and Nanostructures
- Osteoarthritis Treatment and Mechanisms
- Advanced Nanomaterials in Catalysis
- Lipid Membrane Structure and Behavior
- Spaceflight effects on biology
- Rheumatoid Arthritis Research and Therapies
Sanford Burnham Prebys Medical Discovery Institute
2015-2024
Discovery Institute
2015-2024
University of Rome Tor Vergata
2015-2024
Universidade de Ribeirão Preto
2022-2023
Universidade de São Paulo
2022-2023
National Center for Nanoscience and Technology
2018-2019
Innova (Italy)
2019
Chinese Academy of Sciences
2018
La Jolla Institute for Immunology
2014
Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali di Frascati
2005-2013
Investigation of the nanoparticle protein corona, shell plasma proteins formed around nanoparticles immediately after they enter bloodstream, is a benchmark in study applications all fields medicine, from pharmacology to toxicology. We report first investigation corona adsorbed onto single-walled carbon nanotubes modified with 2 kDa molecular weight polyethylene glycol chains [PEG(2k)-modified SWCNTs or PEG2-SWCNTs] by using large-scale gel-based proteomics method on biological replicates....
Rheumatoid arthritis (RA) is one of the most common chronic autoimmune diseases. Despite considerable advances in clinical treatment RA, suboptimal response to therapy and discontinuation are still unresolved challenges due systemic toxicity. It crucial importance actively target deliver therapeutic agents inflamed joints order promote situ activity decrease In this study, we found that SPARC (secreted protein acidic rich cysteine) was overexpressed synovial fluid synovium RA patients as...
Fluorescent nanoparticles were isolated from both pristine and nitric acid-oxidized commercially available carbon nanotubes that had been produced by an electric arc method. The oxidized nanotube-derived fluorescent exhibited a molecular-weight-dependent photoluminescence in the violet-blue blue to yellowish-green ranges, respectively. molecular weight dependency of was strongly related specific supplier. We analyzed composition morphology derived one found materials mainly composed calcium...
Single wall carbon nanotubes (SWCNTs) are considered promising nanoparticles for industrial and biomedical applications; however their potential toxicity in several biological systems, including the feto-placental unit, has been demonstrated. Functionalization of SWCNTs with polyethylene glycol chains (PEG-SWCNTs) dramatically reduces toxicity, this reason PEG-SWCNTs candidates applications. However, no data available on safety developing embryo, spite clinical social relevance topic. The...
Abstract Herein, carbon dot (CD)‐supported Fe single‐atom nanozymes with high content of pyrrolic N and ultrasmall size (ph‐CDs‐Fe SAzyme) are fabricated by a phenanthroline‐mediated ligand‐assisted strategy. Compared phenanthroline‐free (CDs‐Fe SAzyme), ph‐CDs‐Fe SAzyme exhibit higher peroxidase (POD)‐like activity due to their structure similar that ferriporphyrin in natural POD. Aberration‐corrected high‐angle annular dark field scanning transmission electron microscopy (HAADF‐STEM) X‐ray...
Recent evidence regarding the role of regulatory T cells (Treg) in tumor development has suggested that manipulation Treg function selectively microenvironment would be a desirable immunotherapy approach. Targeting intratumor immune populations reduce side effects on peripheral healthy and increase antitumor efficacy immunotherapies. However, no current approaches are available which enable selective vivo targeting or other cell subpopulations. Herein, we investigated ability ligands against...
Osteoarthritis (OA) is a common and debilitating degenerative disease of articular joints for which no disease-modifying medical therapy currently available. Inefficient delivery pharmacologic agents into cartilage-resident chondrocytes after systemic administration has been limitation to the development anti-OA medications. Direct intra-articular injection enables high concentrations in close proximity chondrocytes; however, efficacy this approach limited by fast clearance small molecules...
Abstract Tumor adaptive treatment tolerance associated with chemotherapy originates from low tumor accumulation and adverse effects remains a formidable challenge for cancer therapy. Herein, human serum albumin (HSA)‐based nanomedicines modified diazirine loaded indocyanine green (ICG) tirapazamine (TPZ), denoted as ICG/TPZ@HSA dNMs are developed. The obtained can efficiently eradicate the tumors through cascade of synergistic events triggered by sequential irradiation lasers in area. Upon...
Efficient small interfering RNA (siRNA) delivery in the presence of serum is crucial importance for effective gene therapy. Fluorinated vectors are considered to be attractive candidates siRNA-mediated therapy because their efficacy serum-containing media. However, mechanisms driving superior transfection behavior fluorinated still not well-understood, and comprehensive investigations warranted. Herein, we fabricated a library perfluorooctanoyl fluoride-fluorinated (PFF-fluorinated)...
Abstract Carbon-based nanomaterials including carbon nanotubes (CNTs) have been shown to trigger inflammation. However, how these materials are ‘sensed’ by immune cells is not known. Here we compared the effects of two carbon-based nanomaterials, single-walled CNTs (SWCNTs) and graphene oxide (GO), on primary human monocyte-derived macrophages. Genome-wide transcriptomics assessment was performed at sub-cytotoxic doses. Pathway analysis microarray data revealed pronounced chemokine-encoding...
The adaptive treatment tolerance (ATT) of cancer cells is the main encumbrance to chemotherapy. A potential solution this problem treat with multiple drugs using nanoparticles (NPs). In study, we tested co-administration curcumin (Cur) and doxorubicin (Dox) MCF-7 resistant breast block ATT elicit efficient cell killing. Drugs were co-administered both sequentially simultaneously. Sequential drug was carried out by pre-treating albumin (ANPs) loaded Cur (Cur@ANPs) followed Dox-loaded ANPs...
It remains an unresolved challenge to achieve spatial and temporal monitoring of drug release from nanomedicines (NMs) in vivo, which is crucial importance disease treatment. To tackle this issue, we constructed core-satellite ICG/DOX@Gel-CuS NMs, consist gelatin (Gel) nanoparticles (NPs) with payloads near-infrared fluorochrome indocyanine green (ICG) chemo-drug doxorubicin (DOX) surrounding CuS NPs. The fluorescence ICG was initially shielded by satellite NPs within the intact NMs...
Abstract Our previous research revealed a key microRNA signature that is associated with spaceflight can be used as biomarker and to develop countermeasure treatments mitigate the damage caused by space radiation. Here, we expand on this work determine biological factors rescued treatment. We performed RNA-sequencing transcriptomic analysis 3D microvessel cell cultures exposed simulated deep radiation (0.5 Gy of Galactic Cosmic Radiation) without antagonists three microRNAs: miR-16-5p,...
We describe a novel tunable approach for the synthesis of carbon nanotube-silica nanobead composites. The control nanotube morphology and bead size coupled with versatility silica chemistry makes these structures an excellent platform development biosensors, or optical, magnetic catalytic applications.
We report the formation of a supramolecular luminescent nanoassembly composed individual or small ropes full-length, single-walled carbon nanotubes decorated with streptavidin-conjugated quantum dots. The was stably dispersed under physiological conditions and readily visible by both optical confocal fluorescent microscopies. Jurkat T leukemia cells were able to internalize multivalent CD3 receptor-mediated endocytosis (adsorption cell). Once internalized cells, found be transported...
Single-walled carbon nanotubes (SWCNTs) coated or functionalized with PEG chains of different molecular weight were assessed for their propensity to undergo biodegradation under in vitro conditions using recombinant myeloperoxidase (MPO) ex vivo freshly isolated primary human neutrophils. Our findings suggest that natural conditions, a combined process 'stripping' (i.e., defunctionalization) and PEG-SWCNTs might occur are promising – degradable nanomedicine vector.