A5012592139- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Monoclonal and Polyclonal Antibodies Research
- Antimicrobial Resistance in Staphylococcus
- Virus-based gene therapy research
- Plant Virus Research Studies
- Viral Infections and Immunology Research
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Biochemical and Structural Characterization
- Advanced Electron Microscopy Techniques and Applications
- Extracellular vesicles in disease
- Microbial Metabolism and Applications
- Electron and X-Ray Spectroscopy Techniques
- Virology and Viral Diseases
- Viral Infections and Vectors
- Insect and Arachnid Ecology and Behavior
- Insect and Pesticide Research
- Microbial Community Ecology and Physiology
- Advanced Proteomics Techniques and Applications
- Peptidase Inhibition and Analysis
University of Alabama at Birmingham
2016-2025
University of Alabama
2006-2017
Czech Academy of Sciences, Institute of Microbiology
2013
St. Vincent's Birmingham
2012
Institute of Molecular and Cell Biology
2002-2005
National University of Singapore
1999-2003
Purdue University West Lafayette
1995-1999
University of Oslo
1990-1995
European Molecular Biology Laboratory
1992-1995
European Molecular Biology Laboratory
1993-1994
Norwalk virus, a noncultivatable human calicivirus, is the major cause of epidemic gastroenteritis in humans. The first x-ray structure calicivirus capsid, which consists 180 copies single protein, has been determined by phase extension from low-resolution electron microscopy structure. capsid protein protruding (P) domain connected flexible hinge to shell (S) that classical eight-stranded β-sandwich motif. P unlike any other viral with subdomain exhibiting fold similar second eukaryotic...
Food-derived exosome-like nanoparticles pass through the intestinal tract throughout our lives, but little is known about their impact or function. Here, as a proof of concept, we show that cells targeted by grape (GELNs) are stem whose responses underlie GELN-mediated tissue remodeling and protection against dextran sulfate sodium (DSS)-induced colitis. This finding further supported fact coculturing crypt sorted Lgr5+ with GELNs markedly improved organoid formation. GELN lipids play role...
Abstract BACKGROUND Human seminal fluid contains small exosome‐like vesicles called prostasomes. Prostasomes have been reported previously to play an important role in the process of fertilization by boosting survivability and motility spermatozoa, addition modulating acrosomal reactivity. also present with sizes varying from 50 500 nm multilayered lipid membranes; however, fine morphology prostasomes has never studied detail. METHODS Sucrose gradient‐purified were visualized cryo‐electron...
Chronic inflammation involving both innate and adaptive immune cells is implicated in the pathogenesis of asthma. Intercellular communication essential for driving resolving inflammatory responses Emerging studies suggest that extracellular vesicles (EVs) including exosomes facilitate this process. In report, we have used a range approaches to show EVs contain markers mitochondria derived from donor which are capable sustaining membrane potential. Further, propose these participate...
Mutations in the leucine-rich repeat kinase 2 (LRRK2) gene cause late-onset Parkinson's disease (PD). Emerging evidence suggests a role for LRRK2 endocytic pathway. Here, we show that is released extracellular microvesicles (i.e. exosomes) from cells natively express LRRK2. localizes to collecting duct epithelial kidney actively secrete exosomes into urine. Purified urinary contain protein both dimerized and phosphorylated. We provide quantitative proteomic profile of 1673 proteins find...
Staphylococcus aureus is a common cause of infections in humans. The emergence virulent, antibiotic-resistant strains S. significant public health concern. Most virulence and resistance factors are encoded by mobile genetic elements, transduction bacteriophages represents the main mechanism for horizontal gene transfer. baseplate specialized structure at tip bacteriophage tails that plays key roles host recognition, cell wall penetration, DNA ejection. We have used high-resolution...
Porcine reproductive and respiratory virus (PRRSV) is an enveloped positive-sense RNA of the family Arteriviridae that causes severe persistent disease in pigs worldwide. The PRRSV virion consists a lipid envelope contains several proteins surrounding nucleocapsid core encapsidates genome. To provide better understanding structure assembly PRRSV, we have carried out cryo-electron microscopy tomographic reconstruction virions grown MARC-145 cells. are pleomorphic, round to egg-shaped...
The human immunodeficiency virus (HIV) envelope (Env) protein is incorporated into HIV virions or virus-like particles (VLPs) at very low levels compared to the glycoproteins of most other enveloped viruses. To test factors that influence Env particle incorporation, we generated a series chimeric gene constructs in which coding sequences for signal peptide (SP), transmembrane (TM), and cytoplasmic tail (CT) domains HIV-1 were replaced with those viral cellular proteins individually...
Mumps virus (MuV) is a highly contagious pathogen, and despite extensive vaccination campaigns, outbreaks continue to occur worldwide. The has negative-sense, single-stranded RNA genome that encapsidated by the nucleocapsid protein (N) form (NC). NC serves as template for both transcription replication. In this paper we solved an 18-Å-resolution structure of authentic MuV using cryo-electron microscopy. We also observed effects phosphoprotein (P) binding on structure. N-terminal domain P...
Staphylococcus aureus pathogenicity islands (SaPIs), such as SaPI1, exploit specific helper bacteriophages, like 80α, for their high frequency mobilization, a process termed ‘molecular piracy’. SaPI1 redirects the helper’s assembly pathway to form small capsids that can only accommodate smaller genome, but not complete phage genome. encodes two proteins, CpmA and CpmB, are responsible this size redirection. We have determined structures of 80α procapsids near-atomic resolution by...
Mycobacterium tuberculosis secretes the necrotizing toxin (TNT) to kill host cells. Here, we show that WXG100 proteins EsxE and EsxF are essential for TNT secretion. form a water-soluble heterodimer (EsxEF) assembles into oligomers long filaments, binds membranes, forms stable membrane-spanning channels. Electron microscopy of EsxEF reveals mainly pentameric structures with central pore. Mutations both WXG motifs GXW motif do not affect dimerization, but abolish pore formation, membrane...