A5091280121- Influenza Virus Research Studies
- Respiratory viral infections research
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Advancements in Transdermal Drug Delivery
- Viral gastroenteritis research and epidemiology
- Neonatal Respiratory Health Research
- HIV Research and Treatment
- Dermatology and Skin Diseases
- SARS-CoV-2 and COVID-19 Research
- Animal Disease Management and Epidemiology
- Ginseng Biological Effects and Applications
- Pneumonia and Respiratory Infections
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- vaccines and immunoinformatics approaches
- T-cell and B-cell Immunology
- Heavy Metal Exposure and Toxicity
- Animal Virus Infections Studies
- Inhalation and Respiratory Drug Delivery
- Probiotics and Fermented Foods
- interferon and immune responses
- Diabetes and associated disorders
Georgia State University
2016-2025
Animal and Plant Quarantine Agency
2015
Emory University
2004-2013
Georgia Institute of Technology
2010
National Center for Immunization and Respiratory Diseases
2010
Centers for Disease Control and Prevention
2010
Cardiff University
2010
National Taiwan University
2008
Daegu University
2007
University of California, San Francisco
2007
ABSTRACT Recurrent outbreaks of highly pathogenic avian influenza virus pose the threat pandemic spread lethal disease and make it a priority to develop safe effective vaccines. Influenza virus-like particles (VLPs) have been suggested be promising vaccine approach. However, VLP-induced immune responses, their roles in inducing memory responses cross-protective immunity not investigated. In this study, we developed VLPs containing A/PR8/34 (H1N1) hemagglutinin (HA) matrix (M1) proteins...
Current influenza vaccines provide limited protection against circulating A viruses. universal vaccine will eliminate the intrinsic limitations of seasonal flu vaccines. Here we report methodology to generate double-layered protein nanoparticles as a vaccine. Layered are fabricated by desolvating tetrameric M2e into nanoparticle cores and coating these crosslinking headless HAs. Representative HAs two HA phylogenetic groups constructed purified. Vaccinations with resulting in mice induces...
Background Current influenza vaccines based on the hemagglutinin protein are strain specific and do not provide good protection against drifted viruses or emergence of new pandemic strains. An vaccine that can confer cross-protection antigenically different A strains is highly desirable for improving public health. Methodology/Principal Findings To develop a cross protective vaccine, we generated virus-like particles containing conserved M2 in membrane-anchored form (M2 VLPs), investigated...
Lactobacillus plantarum DK119 (DK119) isolated from the fermented Korean cabbage food was used as a probiotic to determine its antiviral effects on influenza virus. intranasal or oral administration conferred 100% protection against subsequent lethal infection with A viruses, prevented significant weight loss, and lowered lung viral loads in mouse model. The protective efficacy observed dose route dependent manner of administration. Mice that were treated showed high levels cytokines IL-12...
The extracellular domain of M2 (M2e), a small ion channel membrane protein, is well conserved among different human influenza A virus strains. To improve the protective efficacy M2e vaccines, we genetically engineered tandem repeat epitope sequences (M2e5x) human, swine, and avian origin viruses, which was expressed in membrane-anchored form incorporated virus-like particles (VLPs). M2e5x protein with transmembrane hemagglutinin (HA) effectively into VLPs at several 100-fold higher level...
Development of an influenza vaccine that provides broadly cross-protective immunity has been a scientific challenge for more than half century. This study presents approach to overcome strain-specific protection by supplementing conventional vaccines with virus-like particles (VLPs) containing the conserved M2 protein (M2 VLPs) in absence adjuvants. We demonstrate inactivated supplemented VLPs prevents disease symptoms without showing weight loss and confers complete cross against lethal...
Influenza virus-like particles (VLPs) are a promising cell culture-based vaccine, and the skin is considered an attractive immunization site. In this study, we examined immunogenicity protective efficacy of influenza VLPs (H1N1 A/PR/8/34) after vaccination using vaccine dried on solid microneedle arrays. Coating microneedles with unstabilized formulation was found to decrease hemagglutinin (HA) activity, whereas inclusion trehalose disaccharide preserved HA activity VLP vaccines were coated....
Background. Respiratory syncytial virus (RSV) is the leading cause of bronchiolitis and viral death in infants. Despite decades research with traditional or subunit vaccine approaches, there are no approved RSV vaccines. New approaches therefore urgently needed to develop effective Methods. We developed viruslike particles (VLPs) consisting an influenza matrix (M1) protein core RSV-F -G on surface. tested immunogenicity efficacy these VLPs (RSV-F, RSV-G) a mouse model. Results. Intramuscular...
Influenza is a significant health concern worldwide. Viral infection induces local and systemic activation of the immune system causing attendant changes in metabolism. High-resolution metabolomics (HRM) uses advanced mass spectrometry computational methods to measure thousands metabolites inclusive most metabolic pathways. We used HRM identify pathways clusters association related inflammatory cytokines lungs mice with H1N1 influenza virus infection. Infected showed progressive weight loss,...
Lactic acid bacteria (LAB) are the common probiotics. Here, we investigated antiviral protective effects of heat-killed LAB strain Lactobacillus casei DK128 (DK128) on influenza viruses. Intranasal treatment mice with conferred protection against different subtypes viruses by lessening weight loss and lowering viral loads. Protection via was correlated an increase in alveolar macrophage cells lungs airways, early induction virus specific antibodies, reduced levels pro-inflammatory cytokines...
Significance A noninvasive intranasal (i.n.) influenza vaccine can induce mucosal immune responses in respiratory tracts, preventing infection at the portal of virus entry. However, absence appropriate adjuvants present hinders development such a vaccine. Here, we developed polyethyleneimine-functionalized two-dimensional graphene oxide nanoparticles (GP) that showed high antigen-loading capacities and superior immunoenhancing properties. Robust broadly reactive were induced with i.n....
Recovery from live influenza virus infection is known to induce heterosubtypic immunity. In contrast, immunity induced by inactivated vaccines predominantly subtype specific. this study, we investigated the protective virus. Intranasal immunization of mice with A/PR8 (H1N1) provided complete protection against homologous and a drift within same subtype, A/WSN (H1N1), but not A/Philippines (H3N2). However, coadministration cholera toxin as an adjuvant conferred protection, without observed...
ABSTRACT We have designed a membrane-anchored form of the Toll-like receptor 5 ligand flagellin, major proinflammatory determinant enteropathogenic Salmonella , which was found to be glycosylated and expressed on cell surfaces. A chimeric influenza virus-like particle (cVLP) vaccine candidate containing A/PR8/34 (H 1 N ) hemagglutinin (HA), matrix protein (M1), modified flagellin as molecular adjuvant produced. The immunogenicity, including serum antibody levels cellular immune responses,...
The initiation of human immunodeficiency virus type 1 reverse transcription occurs by extension a tRNA(Lys3) primer bound near the 5' end viral RNA genome which is designated binding site (PBS). Sequences within upstream PBS are complementary to anticodon loop (USUU) and T psi C arm (AGGGTm psi) postulated play role in maintaining selective use transcription. To investigate this possibility, proviral genomes contain 3'-terminal 18 nucleotides tRNA(His) [pHXB2(His)] as well sequences either...
Morbidity and mortality due to influenza could be reduced by improved vaccination.To develop a novel skin delivery method that is simple allows for easy self-administration, we prepared microneedle patches with stabilized vaccine investigated their protective immune responses.Mice vaccinated single dose of trehalose-stabilized developed strong antibody responses were long-lived. Compared traditional intramuscular vaccination, vaccination was superior in inducing immunity, as evidenced...
Recurrent outbreaks of highly pathogenic H5N1 avian influenza virus pose a threat eventually causing pandemic. Early vaccination the population would be single most effective measure for control an emerging pandemic.Influenza virus-like particles (VLPs) produced in insect cell-culture substrates do not depend on availability fertile eggs vaccine manufacturing. We VLPs containing A/Viet Nam1203/04 (H5N1) hemagglutinin, neuraminidase, and matrix proteins, investigated their preclinical...
The 2009 influenza pandemic and shortages in vaccine supplies worldwide underscore the need for new approaches to develop more effective vaccines.We generated virus-like particles (VLPs) containing proteins derived from A/California/04/2009 virus, tested their efficacy as a mice. A single intramuscular vaccination with VLPs provided complete protection against lethal challenge virus partial A/PR/8/1934 an antigenically distant human isolate. VLP induced predominant IgG2a antibody responses,...
Simple and effective vaccine administration is particularly important for annually recommended influenza vaccination. We hypothesized that delivery to the skin using a patch containing vaccine-coated microneedles could be an attractive approach improve vaccination compliance efficacy.Solid microneedle arrays coated with inactivated were prepared simple skin. However, stability of vaccine, as measured by hemagglutination activity, was found significantly damaged during coating. The addition...