A5032746548- Metabolomics and Mass Spectrometry Studies
- Redox biology and oxidative stress
- Heavy Metal Exposure and Toxicity
- Sulfur Compounds in Biology
- Health, Environment, Cognitive Aging
- Air Quality and Health Impacts
- Mitochondrial Function and Pathology
- Nitric Oxide and Endothelin Effects
- Glutathione Transferases and Polymorphisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Neonatal Respiratory Health Research
- Genomics, phytochemicals, and oxidative stress
- Trace Elements in Health
- Eicosanoids and Hypertension Pharmacology
- Nutritional Studies and Diet
- Advanced Chemical Sensor Technologies
- Advanced Proteomics Techniques and Applications
- Birth, Development, and Health
- Selenium in Biological Systems
- Heat shock proteins research
- Liver Disease Diagnosis and Treatment
- Respiratory viral infections research
- Antioxidant Activity and Oxidative Stress
- Adipose Tissue and Metabolism
Emory University
2016-2025
Pulmonary and Allergy Associates
2009-2020
Jones College
2016-2019
Unifor
2019
Walker (United States)
2016-2019
Uniformed Services University of the Health Sciences
2019
Khan Academy
2019
Phipps Houses
2019
Pulmonary Associates
2010
Vanderbilt University Medical Center
2008
The exposome is the cumulative measure of environmental influences and associated biological responses throughout lifespan, including exposures from environment, diet, behavior, endogenous processes. A major challenge for research lies in development robust affordable analytic procedures to broad range biologic impacts occurring over a lifetime. Biomonitoring an established approach evaluate internal body burden exposures, but use biomonitoring often limited by high costs with quantification...
Redox mechanisms function in control of gene expression, cell proliferation, and apoptosis, but the circuitry for redox signaling remains unclear. Cysteine methionine are only amino acids proteins that undergo reversible oxidation/reduction under biologic conditions and, as such, provide a means protein activity, protein-protein interaction, trafficking, protein-DNA interaction. Hydrogen peroxide other reactive oxygen species (ROS) mechanism to oxidize proteins. However, oxidation...
Shear stress differentially regulates production of many vasoactive factors at the level gene expression in endothelial cells that may be mediated by mitogen-activated protein kinases, including extracellular signal-regulated kinase (ERK) and N-terminal Jun (JNK). Here we show, using bovine aortic (BAEC), shear ERK JNK mechanisms involving Gi2 pertussis toxin (PTx)-insensitive G-protein-dependent pathways, respectively. activated with a rapid, biphasic time course (maximum 5 min basal 30-min...
Abstract Summary Integrative omics is a central component of most systems biology studies. Computational methods are required for extracting meaningful relationships across different layers. Various tools have been developed to facilitate integration paired heterogenous data; however existing allow only two datasets. Furthermore, data do not incorporate additional steps identifying sub-networks or communities highly connected entities and evaluating the topology integrative network under...
Cadmium (Cd) is present in food at low levels and accumulates humans throughout life because it not effectively excreted. Cd from smoking or occupational exposure shows adverse effects on health, but the mechanistic effect of dietary intake poorly studied. Epidemiology studies found that nonalcoholic fatty liver disease (NAFLD), common U.S. adults, associated with burden. In cell studies, we environmental low-dose oxidized proteins stimulated inflammatory signaling. However, little known...
Influenza is a significant health concern worldwide. Viral infection induces local and systemic activation of the immune system causing attendant changes in metabolism. High-resolution metabolomics (HRM) uses advanced mass spectrometry computational methods to measure thousands metabolites inclusive most metabolic pathways. We used HRM identify pathways clusters association related inflammatory cytokines lungs mice with H1N1 influenza virus infection. Infected showed progressive weight loss,...
Shear stress, the dragging force generated by fluid flow, differentially activates extracellular signal-regulated kinase (ERK) and c-Jun NH<sub>2</sub>-terminal (JNK) in bovine aortic endothelial cells (BAEC) (Jo, H., Sipos, K., Go, Y. M., Law, R., Rong, J., McDonald, J. M. (1997) <i>J. Biol. Chem</i>. 272, 1395–1401). Here, we examine whether cholesterol-enriched compartments plasma membrane are responsible for such differential regulation. Pretreatment of BAEC with a cholesterol-binding...
The c-Jun NH 2 -terminal kinase (JNK), also known as stress-activated protein kinase, is a mitogen-activated that determines cell survival in response to environmental stress. Activation of JNK involves redox-sensitive mechanisms and physiological stimuli such shear stress, the dragging force generated by blood flow over endothelium. Laminar stress has antiatherogenic properties controls structure function endothelial cells including production nitric oxide (NO) superoxide ([Formula: see...
Exogenously added ROS (reactive oxygen species) cause generalized oxidation of cellular components, whereas endogenously generated induced by physiological stimuli activate discrete signal transduction pathways. Compartmentation is an important aspect such pathways, but little known about its role in redox signalling. We measured the states cytosolic and nuclear Trx1 (thioredoxin-1) mitochondrial Trx2 (thioredoxin-2) using Western blot methodologies during endogenous production EGF...
Background— Oxidative stress, a contributing factor to atherosclerosis, causes oxidation of biological thiols, which can be quantified in terms the thiol/disulfide redox. The major redox couple human plasma is cysteine (Cys) and its disulfide, cystine (CySS). Although atherosclerosis has previously been associated with Cys/CySS oxidation, whether contributes causal way development not known. We examined function extracellular potential (E h ) regulation early events using cultured aortic...
Fluid shear stress activates a member of the mitogen-activated protein (MAP) kinase family, extracellular signal-regulated (ERK), by mechanisms dependent on cholesterol in plasma membrane bovine aortic endothelial cells (BAEC). Caveolae are microdomains that enriched with cholesterol, caveolin, and signaling molecules. We hypothesized caveolin-1 regulates activation ERK. Because is not exposed to outside, were minimally permeabilized Triton X-100 (0.01%) deliver neutralizing, polyclonal...