A5047730309- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Viral gastroenteritis research and epidemiology
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- IL-33, ST2, and ILC Pathways
- Immune responses and vaccinations
- Influenza Virus Research Studies
- Dermatology and Skin Diseases
- Psoriasis: Treatment and Pathogenesis
- Probiotics and Fermented Foods
- Dietary Effects on Health
- Diet and metabolism studies
- Inflammatory Bowel Disease
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- Immunotherapy and Immune Responses
- Diet, Metabolism, and Disease
- Long-Term Effects of COVID-19
- SARS-CoV-2 and COVID-19 Research
- Pediatric health and respiratory diseases
- Cancer Cells and Metastasis
- Renal Diseases and Glomerulopathies
- Phagocytosis and Immune Regulation
- Health, psychology, and well-being
Georgia State University
2015-2024
Lactobacillus plantarum DK119 (DK119) isolated from the fermented Korean cabbage food was used as a probiotic to determine its antiviral effects on influenza virus. intranasal or oral administration conferred 100% protection against subsequent lethal infection with A viruses, prevented significant weight loss, and lowered lung viral loads in mouse model. The protective efficacy observed dose route dependent manner of administration. Mice that were treated showed high levels cytokines IL-12...
IL-1 family members are central mediators of host defense. In this article, we show that the novel member IL-36γ was expressed during experimental colitis and human inflammatory bowel disease. Germ-free mice failed to induce in response dextran sodium sulfate (DSS)-induced damage, suggesting gut microbiota involved its induction. Surprisingly, IL-36R-deficient (Il1rl2(-/-)) exhibited defective recovery following DSS-induced damage impaired closure colonic mucosal biopsy wounds, which...
The gut epithelium acts to separate host immune cells from unrestricted interactions with the microbiota and other environmental stimuli. In response epithelial damage or dysfunction, are activated produce interleukin (IL)-22, which is involved in repair protection of barrier surfaces. However, specific pathways leading IL-22 associated antimicrobial peptide (AMP) production intestinal tissue remain incompletely understood. Here, we define a critical IL-36/IL-23/IL-22 cytokine network that...
Lactic acid bacteria (LAB) are the common probiotics. Here, we investigated antiviral protective effects of heat-killed LAB strain Lactobacillus casei DK128 (DK128) on influenza viruses. Intranasal treatment mice with conferred protection against different subtypes viruses by lessening weight loss and lowering viral loads. Protection via was correlated an increase in alveolar macrophage cells lungs airways, early induction virus specific antibodies, reduced levels pro-inflammatory cytokines...
The mechanism of how plant-derived nanovesicles are uptaken by cells remains unknown. In this study, the garlic-derived (GDVs) were isolated and digested with trypsin to remove all surface proteins. Digested GDVs showed less uptake compared undigested GDVs, confirming that proteins played a role in endocytosis. On cell side (HepG2), interestingly, blocking CD98 receptors significantly reduced GDVs. During cellular internalization we observed some co-localized CD98. A total lysate GDV high...
Background & AimsNourishment of gut microbiota via consumption fermentable fiber promotes health and guards against metabolic syndrome. In contrast, how dietary impacts type 1 diabetes is less clear.MethodsTo examine impact fibers on development in the streptozotocin (STZ)-induced spontaneous non-obese (NOD) models, mice were fed grain-based chow (GBC) or compositionally defined diets enriched with a (inulin) an insoluble (cellulose). Spontaneous (NOD mice) STZ-induced (wild-type was...
Regulatory and effector T helper (Th) cells are abundant at mucosal surfaces, especially in the intestine, where they control critical balance between tolerance inflammation. However, key factors that reciprocally dictate differentiation along these specific lineages remain incompletely understood. Here we report interleukin-1 (IL-1) family member IL-36γ signals through IL-36 receptor, myeloid primary response gene 88, nuclear factor-κBp50 CD4+ to potently inhibit Foxp3-expressing induced...
Background & AimsFiber-rich foods promote health, but mechanisms by which they do so remain poorly defined. Screening fiber types, in mice, revealed psyllium had unique ability to ameliorate 2 chronic inflammatory states, namely, metabolic syndrome and colitis. We sought determine the mechanism of action latter.MethodsMice were fed grain-based chow, is naturally rich or compositionally defined diets enriched with semi-purified fibers. Mice studied basally models chemical-induced T-cell...
Abstract Gut microbiota and their metabolites are instrumental in regulating intestinal homeostasis. However, early-life associated influences on development remain incompletely understood. Here we demonstrate that co-housing of germ-free (GF) mice with specific-pathogen free (SPF) at weaning (exGF) results altered gene expression. Our reveal one highly differentially expressed gene, erythroid differentiation regulator-1 (Erdr1), is induced during SPF but not GF or exGF localizes to Lgr5 +...
The most severe outcomes of respiratory viral infection (RVI) result from secondary bacterial infection, which RVI promotes via depletion alveolar macrophages (AM). Colonization the intestine by common but non-ubiquitous commensal, segmented filamentous bacteria (SFB), reprograms AM to resist RVI-induced depletion. Hence, we examined if SFB against S. pneumoniae, H. influenzae, or aureus, following primary influenza virus (IAV). Indeed, colonization conferred strong post-IAV protection these...
Reduced dietary fiber intake is associated with, and may have contributed to, the post-mid-20th century increase in immune-mediated chronic inflammatory diseases, including bowel disease. has resulted, part, from highly refined foods. For example, modern methods of producing bread removes much naturally present wheat kernels. Accordingly, we hypothesized that might protect against diseases. We tested this notion a murine T-cell transfer colitis model. Rag1-/- mice were fed open-source...
The physiological role of T cell anergy induction as a key mechanism supporting self-tolerance remains undefined, and natural antigens that induce are largely unknown. In this report, we used TCR sequencing to show the recruitment CD4+CD44+Foxp3-CD73+FR4+ anergic (Tan) cells expands CD4+Foxp3+ (Tregs) repertoire. Next, report blockade in peripherally-induced Tregs (pTregs) formation due mutation CNS1 region Foxp3 or chronic exposure selecting self-peptide result an accumulation Tan cells....
Prevention of rotavirus (RV) infection by gut-resident segmented filamentous bacteria (SFB) is an example the influence gut microbiota composition on enteric viral infection. Yet, mechanism which SFB prevents RV poorly understood. A recent report that colonization germfree mice generates retinoic acid (RA) thus activating RA receptor (RAR) signaling, protected against Citrobacter rodentium infection, prompted us to investigate whether this pathway might contribute SFB's protection...
Impacts of dietary fiber on intestinal inflammation are complex, but some specific semi-purified fibers, particularly psyllium, can protect humans and rodents against colitis. Mechanisms underlying such protection not fully understood may involve activation the FXR bile acid receptor. Obesity its associated consequences, referred to as metabolic syndrome, with, promoted by, low-grade in a variety tissues including intestine. Hence, we examined whether psyllium might ameliorate that occurs...
Interleukin (IL)-22 promotes host-microbiota homeostasis. We sought to identify microbiota metabolite(s) that drive intestinal IL-22 production. observed exposing Peyer's patch cells (PPCs), ex vivo, fecal supernatants (FSs) recapitulates fermentable fiber- and microbiota-dependent production, cellular sources thereof, thus supporting the use of this model. An interrogation FSs generated from mice fed fiber inulin (FS-Inu) revealed its IL-22-inducing activity is mediated by heat-labile...
Significance Upon enteropathogenic bacterial infection, cytokines orchestrate innate and adaptive immune activation that promotes barrier protection pathogen control. Using Citrobacter rodentium , we defined a critical role for IL-36R signaling in driving early IL-23- late IL-6-mediated IL-22 production, antimicrobial activity, host protection. Our data show the IL-36/IL-36R axis integrates responses to afford infection. These findings provide mechanistic insight into how proinflammatory...
Interleukin (IL)-2 is expressed during T cell activation and induces the proliferation differentiation of cells. CD4+Foxp3+ regulatory cells (Tregs) constitutively express high affinity IL-2 receptor (CD25/IL-2R) rapidly respond to elaborate numerous suppressive mechanisms that limit immune-mediated pathologies. Accumulating evidence supports concept an aberrant balance between Tregs Teff contribute pathology intestinal inflammation IL-2/Treg axis a potential pathway exploit for treatment...
This study showed that the absence of CCR7 or mesenteric lymph nodes/gut-associated lymphoid tissue did not appreciably impact total intestinal Foxp3+ regulatory T cell representation in steady-state. However, nodes/GALT are required for normal peripherally induced differentiation small intestine, but large intestine.Foxp3+ cells (Tregs) intestine promote immune tolerance to enteric antigens. Previous studies have shown C-C chemokine receptor 7 (CCR7)-dependent migration dendritic nodes...
The immunocompromised are at high risk of prolonged SARS-CoV-2 infection and progression to severe COVID-19. However, efficacy late-onset direct-acting antiviral (DAA) therapy with therapeutics in clinical use experimental drugs mitigate persistent viral replication is unclear. In this study, we employed an mouse model, which supports explore treatment options. Tandem immuno-depletion CD4