A5101954421- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Magnetic properties of thin films
- Surface and Thin Film Phenomena
- Gold and Silver Nanoparticles Synthesis and Applications
- Plasmonic and Surface Plasmon Research
- Proteins in Food Systems
- Orthopedic Infections and Treatments
- Nanoplatforms for cancer theranostics
- Total Knee Arthroplasty Outcomes
- Extracellular vesicles in disease
- Orthopaedic implants and arthroplasty
- Advanced Materials Characterization Techniques
- Microencapsulation and Drying Processes
- Immunotherapy and Immune Responses
- Biosensors and Analytical Detection
- Botanical Research and Applications
- Copper Interconnects and Reliability
- Theoretical and Computational Physics
- Physics of Superconductivity and Magnetism
- Dendrimers and Hyperbranched Polymers
- Ion-surface interactions and analysis
- Phytochemicals and Antioxidant Activities
- Electrospun Nanofibers in Biomedical Applications
National Center for Nanoscience and Technology
2014-2024
Shenyang Agricultural University
2021-2024
University of Chinese Academy of Sciences
2019-2024
Taiyuan University of Technology
2024
Dalian University of Technology
2024
Xiangya Hospital Central South University
2024
Central South University
2024
Chinese Academy of Sciences
2016-2023
Peking University
2014-2022
Peking University First Hospital
2014-2022
Many chemotherapeutics used for cancer treatments encounter issues during delivery to tumors in vivo and may have high levels of systemic toxicity due their nonspecific distribution. Various materials been explored fabricate nanoparticles as drug carriers improve efficiency. However, most these suffer from multiple drawbacks, such limited biocompatibility inability engineer spatially addressable surfaces that can be utilized multifunctional activity. Here, we demonstrate DNA origami...
Charge-reversal functional gold nanoparticles first prepared by layer-by-layer technique were employed to deliver small interfering RNA (siRNA) and plasmid DNA into cancer cells. Polyacrylamide gel electrophoresis measurements of siRNA confirmed the occurrence charge-reversal property nanoparticles. The expression efficiency enhanced green fluorescent protein (EGFP) was improved adjuvant transfection with nanoparticles, which also had much lower toxicity cell proliferation. Lamin A/C, an...
Molecular chemistry offers a unique toolkit to draw inspiration for the design of artificial metamolecules. For long time, optical circular dichroism has been exclusively terrain natural chiral molecules, which exhibit activity mainly in UV spectral range, thus greatly hindering their significance broad range applications. Here we demonstrate that can be generated with plasmonic nanostructures composed minimum number spherical gold nanoparticles required three-dimensional (3D) chirality. We...
The efficient delivery of a therapeutic gene into target tissues has remained major obstacle in realizing viable gene-based medicine. Herein, we introduce facile and universal strategy to construct DNA nanostructure-based codelivery system containing linear tumor (p53) chemotherapeutic drug (doxorubicin, DOX) for combined therapy multidrug resistant (MCF-7R). This novel system, which is structurally similar kite, rationally designed contain multiple functional groups the targeted controlled...
A functional cancer theranostic nanoplatform is developed, specifically tailored toward the optoacoustic modality by combining gold nanorods with DNA nanostructures (D–AuNR). origami used as an efficient delivery vehicle owing to its prominent tumor-targeting property. The D–AuNR hybrids display enhanced tumor diagnostic sensitivity improved imaging and excellent photothermal therapeutic properties in vivo. As a service our authors readers, this journal provides supporting information...
Abstract Multidrug resistance (MDR) is a major obstacle in the clinical treatment of cancer. Herein, facile strategy reported to construct versatile DNA nanostructure as co‐delivery vector RNA interference (RNAi) and chemodrugs combat multidrug‐resistant tumor (MCF‐7R) vitro vivo. In tailored nanocarrier, two linear small hairpin (shRNA) transcription templates targeting MDR‐associated genes (gene P‐glycoprotein, typical drug efflux pump; gene survivin, representative anti‐apoptotic protein)...
Abstract Using the DNA origami technique, we constructed a nanodevice functionalized with small interfering RNA (siRNA) within its inner cavity and chemotherapeutic drug doxorubicin (DOX), intercalated in duplexes. The incorporation of disulfide bonds allows triggered mechanical opening release siRNA response to intracellular glutathione (GSH) tumors knockdown genes key cancer progression. Combining interference chemotherapy, induced potent cytotoxicity tumor growth inhibition, without...
Photodynamic therapy (PDT) offers an alternative for cancer treatment by using ultraviolet or visible light in the presence of a photosensitizer and molecular oxygen, which can produce highly reactive oxygen species that ultimately leading to ablation tumor cells multifactorial mechanisms. However, this technique is limited penetration depth incident light, hypoxic environment solid tumors, vulnerability photobleaching reduces efficiency many imaging agents. In work, we reported cellular...
Abstract Effective and safe hemodialysis is essential for patients with acute kidney injury chronic renal failures. However, the development of effective anticoagulant agents antidotes use during has proven challenging. Here, we describe DNA origami-based assemblies that enable inhibition thrombin activity thrombus formation. Two different thrombin-binding aptamers decorated origami initiates protein recognition inhibition, exhibiting enhanced anticoagulation in human plasma, fresh whole...
The hysteresis loops of epitaxially grown ultrathin Co films on a Cu(001) surface with magnetic anisotropy were measured in situ at room temperature under periodic external field by the magneto-optical Kerr-effect technique. A dynamic phase transition occurs high frequencies. At very low frequencies, \ensuremath{\Omega}, constant loop area exists. We also observed power-law scaling area,...
We present a flexible and highly reproducible method using three-dimensional (3D) multicellular tumor spheroids to quantify chemotherapeutic nanoparticle penetration properties in vitro. generated HeLa cell-derived the liquid overlay method. To properly characterize spheroids, scanning electron microscopy, transmission multiphoton microscopy were used obtain high-resolution 3D images of spheroids. Next, pairing optical characterization techniques with flow cytometry, we quantitatively...
The exploration of biocompatible materials with circularly polarized luminescence (CPL) activity is becoming an attractive topic due to the great potential application in biosensing and bioimaging. Here, we describe a strategy fabricate new CPL-active biomaterials using achiral carbazole-based biscyanine fluorophores coassembled chiral deoxyribonucleic acid (DNA) molecules. This cyanine molecule has been shown behave as DNA detecting probe, featuring strong fluorescent emission induced by...
In response to environmental variations, living cells need arrange the conformational changes of macromolecules achieve specific biofunctions. Inspired by natural molecular machines, artificial macromolecular assemblies with controllable nanostructures and environmentally responsive functions can be designed. By assembling noble metal nanoparticles, information could significantly amplified modulated. However, manufacturing dynamic plasmonic that are efficiently different stimuli is still a...
We herein demonstrate that DNA origami can work as a multifunctional platform integrating chemotherapeutic drug (doxorubicin), gold nanorods and tumour-specific aptamer MUC-1, to realize the effective circumvention of resistance. Doxorubicin (DOX) was loaded efficiently onto through base pair intercalation surface-modified (AuNRs) were assembled hybridization. Due active targeting effect aptamers, nanostructures achieved increased cellular internalization DOX AuNRs. Upon near-infrared (NIR)...
Dendrimer-like DNA nanostructures have attractive properties such as mechanical stability, highly branched nanostructure, customized sizes, and biocompatibility. In this study, we construct programmable dendrimeric nanoparticles efficient vehicles to deliver immunostimulatory cytosine-phosphate-guanosine (CpG) sequences for activation of the immune response. dendrimers decorated with CpG-containing hairpin-loops triggered stronger response characterized by pro-inflammatory cytokines...
Delivery of proteins to carry out desired biological functions is a direct approach for disease treatment. However, protein therapy still facing challenges due low delivery efficiency, poor targeting during trafficking, insufficient therapeutic efficacy, and possible toxicity induced by carriers. Here, we present novel platform based on DNA origami nanostructure that enables tumor cell transportation active cancer therapy. In our design, cytotoxic ribonuclease (RNase) A molecules are...