A5101974440- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Biosensors and Analytical Detection
- S100 Proteins and Annexins
- Endometrial and Cervical Cancer Treatments
- Cancer-related gene regulation
- Pneumothorax, Barotrauma, Emphysema
- Cancer-related Molecular Pathways
- Luminescence and Fluorescent Materials
- Bacteriophages and microbial interactions
- Cancer Research and Treatments
- Gold and Silver Nanoparticles Synthesis and Applications
- COVID-19 Clinical Research Studies
- Plasmonic and Surface Plasmon Research
- Colorectal and Anal Carcinomas
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Epigenetics and DNA Methylation
- Inflammatory Bowel Disease
- Sulfur Compounds in Biology
- NF-κB Signaling Pathways
- Cancer, Hypoxia, and Metabolism
- Pleural and Pulmonary Diseases
- Graphene and Nanomaterials Applications
- COVID-19 and healthcare impacts
Renmin Hospital of Wuhan University
2020-2021
Wuhan University
2018-2021
Nanjing University
2018-2021
East China Normal University
2021
Zhongnan Hospital of Wuhan University
2018-2019
East China University of Science and Technology
2014-2017
Jiangsu University
2017
First Affiliated Hospital of Zhengzhou University
2010
National Cancer Institute
1997
As a milestone in DNA self-assembly, origami has demonstrated powerful applications many fields. However, the scarce availability of long single-stranded (ssDNA) limits size and sequences nanostructures, which turn impedes further development. In this study, we present robust strategy to produce circular ssDNA scaffold strands with custom-tailored lengths sequences. These products were then used as scaffolds for constructing various nanostructures. This scalable method produces at low cost...
Although affinity binding between short chain peptides and carbon nanotube (CNT) has been reported, little is known for the study of proteins with CNT recognition specific capabilities. Herein, carbonic anhydrase (CA) was functionalized via protein fusion a single-walled (SWNTs)-binding peptide, thereby forming bioactive high capability. TEM AFM analyses showed that CA could firmly coat to SWNTs surface coverage over 51%, while enzyme maintained its catalytic activity. Structural analysis...
The manufacture of DNA origami nanostructures with highly ordered functional motifs is great significance for biomedical applications. Here, we present a robust strategy to produce customized scaffolds integrated aptamer sequences, which enables direct construction structures. As demonstrated, aptamers various numbers and types were efficiently stably in user-defined positions the scaffolds. Specifically, two different thrombin sequences simultaneously inserted into M13mp18 phage genome....
The outbreak of the novel coronavirus (COVID-19) that was firstly reported in Wuhan, China, with cases now confirmed more than 100 countries. However, COVID-19 pneumonia spontaneous pneumothorax is unknown.We a case 66-year-old man infected COVID-19, presenting fever, cough and myalgia; patient received supportive empirical treatment including antiviral treatment, anti-inflammatory oxygen supply inhalation therapy; symptoms, CT images, laboratory results got improved after treatments, throat...
DNA nanotechnology is powerful in constructing programmable nanostructures with distinct dimensions, sizes, and shapes. However, natural molecules are prone to nuclease degradation, thus limiting the vivo applications of such nanostructures. 2′-Fluoroarabinonucleic acid (FANA) a chemically modified oligonucleotide similar base pairing properties exhibits superior physical chemical stabilities. In this work, FANA were used construct double crossover nanostructures, it was demonstrated that...
Abstract Global DNA hypomethylation is a most common epigenetic alteration in human neoplasia. However, accumulative evidence shows that global impacts tumorigenesis tissue-specific manner, promoting some but suppressing others including colorectal cancer. The underlying mechanisms, especially how suppresses tumorigenesis, remain largely unknown. Here, we investigate affects intestinal by using an Uhrf1 tandem tudor domain knockin mutant mouse model ( ki/ki ) exhibits moderate ~10% reduction...
Transgenic mice expressing the simian virus 40 large T-antigen (TAG) under regulatory control of rat prostatic steroid binding protein C3(1) gene develop mammary carcinomas (in females) and prostate males). The development occurs several months after appearance dysplastic lesions, suggesting that TAG is necessary but insufficient for tumor formation other genetic events are involved in this process. known to bind p53 result its functional inactivation, which believed be a critical step...
Abstract Myeloid differentiation 1 (MD‐1), also known as lymphocyte antigen 86 (Ly86), is a soluble protein homologous to MD‐2 and forms complex with radioprotective 105 (RP105). RP105/MD‐1 negatively regulates toll‐like receptor 4 (TLR4) signaling involved in several immune disorders. However, the precise role of MD‐1 inflammatory bowel diseases (IBD) remains poorly understood. To further investigate involvement IBD, we inhibited colon antisense oligonucleotide (AS‐ODN) assessed effect...
The aim of this study was to investigate the impact vaginal cuff length (VCL) resected during radical hysterectomy (RH) on long-term survival outcomes patients with cervical cancer (International Federation Gynecology and Obstetrics [FIGO] stage IB-IIA) explore whether VCL is a prognostic factor for cancer.A total 435 eligible IB IIA who underwent RH in Zhongnan Hospital Wuhan University (Wuhan, People's Republic China) from January 2007 February 2017 were retrospectively analyzed. These...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) broke out in Wuhan, China, late December 2019 and has since spread rapidly around the world. disease (COVID-19) pneumonia patients have abnormal blood coagulation function, but their thromboembolism prevalence is still unknown. We reported a case of 49-year-old man infected with COVID-19, presenting fever, chest pain, limb weakness, myalgia, dyspnea. The patient was diagnosed severe COVID-19 pneumonia, pulmonary (PTE), deep vein...
Objective To establish the brain injured models induced by lipopolysaccharide (LPS) in infantile rats, and then, investigate expression changes ofneutrophil elastase (NE) to explore its mechanism. Methods Eighty 1-month-old SD rats were randomly divided into LPS inducement group (injected via external carotid, n=40) control normal saline n=40). Each was equally subdivided 2 subgroups: 24 48 h after injection groups, respectively. Rats drawn blood at different time points detect content of...