A5091036310- Aldose Reductase and Taurine
- COVID-19 and healthcare impacts
- Economic and Financial Impacts of Cancer
- interferon and immune responses
- Inflammatory Biomarkers in Disease Prognosis
- Pneumonia and Respiratory Infections
- Genetic factors in colorectal cancer
- Metabolism, Diabetes, and Cancer
- Immune Response and Inflammation
- Blood disorders and treatments
- Heat shock proteins research
- Immune cells in cancer
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Esophageal Cancer Research and Treatment
- Cancer Immunotherapy and Biomarkers
- Cytokine Signaling Pathways and Interactions
- RNA and protein synthesis mechanisms
- Cancer Genomics and Diagnostics
- Lung Cancer Treatments and Mutations
- COVID-19 Clinical Research Studies
- Reproductive tract infections research
- Prenatal Substance Exposure Effects
- Advanced Glycation End Products research
University of Chinese Academy of Sciences
2020-2023
Chinese Academy of Sciences
2020-2023
Zhejiang Cancer Hospital
2020-2023
Hangzhou Cancer Hospital
2023
Cancer Hospital of Chinese Academy of Medical Sciences
2020-2023
Zhejiang Academy of Social Sciences
2020
Sir Run Run Shaw Hospital
2014-2018
Zhejiang University
2014-2018
Chinese University of Hong Kong
2008-2012
National Institutes of Health
2011-2012
Polyphyllin D (PD) is a potent cytotoxic saponin found in Paris polyphylla. In the present study, bioinformatic, proteomic and transcriptomic analyses were performed to study mechanisms of action PD on human nonsmall cell lung cancer (NSCLC) line (NCI-H460). Using gene expression-based bioinformatic tool (connectivity map), was identified as potential ER stress inducer. Our revealed that treatment led upregulation typical stress-related proteins/genes including glucose-regulated protein 78...
Macrophages, when activated by IFN-γ and TLR signaling, elicit innate immune responses. IFN regulatory factor 8 (IRF8) is a transcription that facilitates macrophage activation immunity. We show that, in resting macrophages, some IRF8 conjugated to small ubiquitin-like modifiers (SUMO) 2/3 through the lysine residue 310. SUMO3-conjugated failed induce IL12p40 other target genes, consistent with SUMO-mediated transcriptional repression reported for factors. showed reduced mobility live nuclei...
Background Previous studies have suggested that the ratios of immune-inflammatory cells could serve as prognostic indicators in ovarian cancer. However, which these is superior indicator cancer remains unknown. In addition, on value platelet to neutrophil ratio (PNR) are still limited. Methods A cohort 991 patients was analyzed present study. Receiver operator characteristic (ROC) curves were utilized choose optimal cut-off values inflammatory biomarkers such lymphocyte (NLR), monocyte...
The osmotic response element-binding protein (OREBP), also known as tonicity enhancer-binding (TonEBP) or NFAT5, is the only osmo-sensitive transcription factor that mediates cellular adaptations to extracellular hypertonic stress. Although it well documented subcellular localization and transactivation activity of OREBP/TonEBP are tightly regulated by tonicity, molecular mechanisms involved remain elusive. Here we show nucleocytoplasmic trafficking dual phosphorylation Ser-155 Ser-158....
The protein inhibitor of activated STAT (PIAS) family proteins regulates innate immune responses by controlling transcription induced Toll-like receptor, RIG-I-like receptor signaling, and JAK/STAT pathways. Here, we show that PIASy negatively type I interferon (IFN) transcription. Virus infection led to enhanced IFN induction in null cells, conversely overexpression reduced A mutation the LXXLL motif SAP domain abolished inhibition IFN-stimulated gene expression but did not affect virus or...
Objectives It is significant to develop effective prognostic strategies and techniques for improving the survival rate of gallbladder carcinoma (GBC). We aim prediction model from multi-clinical indicators combined artificial intelligence (AI) algorithm prognosis GBC. Methods A total 122 patients with GBC January 2015 December 2019 were collected in this study. Based on analysis correlation, relative risk, receiver operator characteristic curve, importance by AI between clinical factors...
To identify Streptococcus pneumoniae genes expressed specifically during infections, a selection system based on the in vivo expression technology (IVET) was established. galU, which is critical for capsular polysaccharide biosynthesis, and lacZY encoding β-galactosidase were employed as dual reporter to screen in-vivo-induced (ivi) of S. pneumoniae. The galU-deficient mutant incapable utilizing galactose, thus failing synthesize polysaccharide, therefore loses its ability survive host. A...
The ATP-dependent caseinolytic proteases (Clp) play a fundamental role in stress tolerance and virulence many pathogenic bacteria. Although ClpE of Streptococcus pneumoniae is required for growth at high temperatures, little known about the pathogenesis. In this study, we observed that clpE mutant S. strain D39 was strongly reduced mouse intraperitoneal infection model. also showed substantially adherence to human lung epithelial carcinoma A549 cell line umbilical-vein-derived endothelial...
In an effort to identify a novel microRNA (miRNA) as gastric cancer (GC) treatment target and prognostic biomarker, we surveyed The Cancer Genome Atlas database found that miR-588 expression is low in GC tissues. This was confirmed by real-time reverse transcription polymerase chain reaction assays of patient plasma samples SGC7901 MNK28 cells. A constructed miRNA-mRNA network showed CXCL5, CXCL9, CXCL10 are genes miR-588. Analysis the miRWalk revealed directly binds CXCL5 CXCL9....
Background Osmotic Response Element-Binding Protein (OREBP), also known as TonEBP or NFAT5, is a unique transcription factor. It hitherto the only mammalian factor that regulates hypertonic stress-induced gene transcription. In addition, unlike other monomeric members of NFAT family, OREBP exists homodimer and it to bind naked DNA targets by complete encirclement in vitro. Nevertheless, how interacts with target DNA, ORE/TonE, elicits vivo, remains unknown. Methodology Using induction aldose...
NFAT5 is the only known mammalian tonicity-responsive transcription factor with an essential role in cellular adaptation to hypertonic stress. It also implicated diverse physiological and pathological processes. activity tightly regulated by extracellular tonicity, but underlying mechanisms remain elusive. Here, we demonstrate that enters nucleus via nuclear pore complex. We found utilizes a unique localization signal (NFAT5-NLS) for import. siRNA screening revealed karyopherin β1 (KPNB1),...
The rapid development of the Internet leads to growth network information, we call it information explosion. is full and difficult for users find this useful knowledge ocean. Web has become world's largest repository, there an urgent need efficient access valuable vast amounts web information. purpose paper study process algorithm analysis text mining system based on artificial intelligence. This presents document feature acquisition genetic algorithm. Selecting suitable features important...
Type I IFNs (IFNIs) are involved in the course of antiviral and antimicrobial activities; however, robust inductions these can lead to host immunopathology. We have reported that Pias (protein inhibitor activated signal transducer activator transcription) family member, Piasy, possesses ability suppress IFNI transcriptions mouse embryonic fibroblasts (MEFs), yet specific molecular mechanism by which it acts remains elusive. Here, we identify H3K4me3 levels, one activation mark genes, MEFs...
Objective: Lens epithelial cell (LEC) membrane damage is one of the pathogenesis cataract. High mobility group box-1 (HMGB-1) and nuclear factor-κB (NF-κB) play vital roles in a variety diseases, such as inflammation. Ketamine has numerous pharmacological effects that can inhibit However, its role cataract rats LECs not yet been elucidated.Materials methods: were isolated from SD cultured vitro. The cells randomly divided into three groups, including control group, model induced by H2O2,...
Due to the increased risk of viral infection and severe shortage medical resources during pandemic COVID-19, most hospitals in epidemic areas significantly reduced non-emergency admissions services, if not closed. As a result, it has been difficult treat cancer patients on time, which adversely affects their prognosis. To address this problem, centers must develop strategic plan manage both inpatients outpatients pandemic, provide them with necessary treatment, at same time prevent spread...
Object: Prostate cancer is one of the leading malignancies in men worldwide.Previous observational studies have linked amino acids and transaminase with altered risk prostate cancer.However, whether these associations were causal remained unclear.Therefore, we conducted a Mendelian randomization (MR) to assess their potential associations.Methods: Summary-level data for obtained from meta-analysis genome-wide association (GWAS) including 79,148 cases 61,106 controls European...
Aims: This study aimed to investigate the relationship between perioperative change in neutrophil count and survival of patients with esophageal squamous cell carcinoma. Method: Neutrophil (Nc) (where Nc = post-surgery − pre-surgery count) was counted according data within 1 week before surgery 2 weeks after surgery. Patients were divided into two groups, ≥2.60 <2.60, median Nc. Results: Multivariate analysis revealed that an independent prognostic marker for overall survival. Subgroup...
Liver kinase B1 (LKB1) is a tumor suppressor gene, the inactivation of which occurs frequently in different types. However, whether LKB1 associated with clinical features gastric cancer (GC) and regulating immunity unknown. In this study, we showed that highly expressed serum healthy individuals (n = 176) compared to GC patients 416) also outcomes good survival rates patients. Furthermore, genes immune checkpoints T cell activation, such as PD-1, PD-L1, CD8A, CD8B, CD28, GZMM, were shown be...
Abstract Background: Due to the increased risk of viral infection and severe shortage medical resources during pandemic COVID-19, most hospitals in epidemic areas significantly reduced non-emergency admissions services, if not closed. As a result, it has been difficult treat cancer patients on time, which adversely affects their prognosis. To address this problem, centers must develop strategic plan manage both inpatients outpatients pandemic, provide them with necessary treatment, at same...