A5101621813- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Lymphoma Diagnosis and Treatment
- Drug Transport and Resistance Mechanisms
- Lung Cancer Treatments and Mutations
- Nanoparticles: synthesis and applications
- RNA modifications and cancer
- Prostate Cancer Treatment and Research
- Estrogen and related hormone effects
- Antibiotic Resistance in Bacteria
- Advanced Fluorescence Microscopy Techniques
- Plasmonic and Surface Plasmon Research
- Acupuncture Treatment Research Studies
- DNA and Nucleic Acid Chemistry
- Nanoparticle-Based Drug Delivery
- Hormonal and reproductive studies
- Protein Degradation and Inhibitors
- Bladder and Urothelial Cancer Treatments
- Cancer Cells and Metastasis
- Hormonal Regulation and Hypertension
- Multiple Myeloma Research and Treatments
- Bacterial biofilms and quorum sensing
- Pregnancy and preeclampsia studies
- Advanced Biosensing Techniques and Applications
- Adrenal Hormones and Disorders
Tianjin Medical University
2015-2025
Shanxi Normal University
2012-2025
Zhejiang Cancer Hospital
2011-2024
Cancer Hospital of Chinese Academy of Medical Sciences
2021-2024
University of Chinese Academy of Sciences
2020-2024
Wenzhou Medical University
2023
Chinese Academy of Sciences
2020-2023
Nantong University
2009-2023
Nantong Tumor Hospital
2009-2023
Second Affiliated Hospital of Zhejiang University
2005-2023
Real-time study of the transport and biocompatibility nanomaterials in early embryonic development at single-nanoparticle resolution can offer new knowledge about delivery effects vivo provide insights into molecular mechanisms developing embryos. In this study, we directly characterized single silver nanoparticles an model system (zebrafish embryos) investigated their on real time. We designed highly purified stable (not aggregated no photodecomposition) developed optics assays to enable...
Noble metal nanoparticles (NPs) possess size- and shape-dependent optical properties, suggesting the possibility of tuning desired properties ensemble NPs at single NP resolution underscoring importance probing sizes shapes in situ real-time. In this study, we synthesized twelve colloids Ag NPs. Each colloid contains various NPs, showing rainbow colors with peak-wavelength absorption spectra from 393 to 738 nm. We correlated determined by high-resolution transmission electron microscopy...
Continuous monitoring of submillisecond free-solution dynamics individual rhodamine-6G molecules and 30-base single-stranded DNA tagged with rhodamine was achieved. Fluorescence images were recorded from the same set isolated excited either through evanescent field at quartz-liquid interface or as a thin layer solution defined by micron-sized wires, giving diffraction-limited resolution interconnected attoliter volume elements. The single-molecule diffusion coefficients smaller unimolecular...
Membrane transport plays a leading role in wide spectrum of cellular and subcellular pathways, including multidrug resistance (MDR), signaling, cell−cell communication. Pseudomonas aeruginosa is renowned for its intriguing membrane mechanisms, such as the interplay permeability extrusion machinery, to selective accumulation specific intracellular substances MDR. Despite extensive studies, mechanisms living microbial cells remain incompletely understood. In this study, we directly measure...
The motion of single, dye-labeled protein molecules was monitored at various pH and ionic strengths within the 180-nanometer-thick evanescent-field layer a fused-silica surface. Below isoelectric point, partitioning into excitation region increased in number but maintained random spatial distribution, implying that surface charge can influence charged distances beyond electrical double-layer thickness. residence times interfacial also below point. However, immobilization on solid for...
We have synthesized and characterized stable (non-aggregating, non-photobleaching non-blinking), nearly monodisperse highly-pure Au nanoparticles, used them to probe nanoparticle transport diffusion in cleavage-stage zebrafish embryos study their effects on embryonic development real-time. found that single nanoparticles (11.6 ± 0.9 nm diameter) passively diffused into the chorionic space of via pore canals continued random-walk through inner mass embryos. Diffusion coefficients vary...
Abstract Reactive oxygen species (ROS) depletion and low ROS production that result from the intratumoral redox metabolism equilibrium energy conversion efficiency ultrasound mechanical to ROS‐represented chemical energy, respectively, are two vital inhibitory factors of sonodynamic therapy (SDT). To address concerns, a tumor metabolism‐engineered composite nanoplatform capable intervening metabolism, breaking equilibrium, reshaping microenvironment is constructed reinforce SDT against...
Nanomaterials possess distinctive physicochemical properties (e.g., small sizes and high surface area-to-volume ratios) promise a wide variety of applications, ranging from the design quality consumer products to effective disease diagnosis therapy. These can lead toxic effects, potentially hindering advances in nanotechnology. In this study, we have synthesized characterized purified stable (nonaggregation) silver nanoparticles (Ag NPs, 41.6 ± 9.1 nm average diameter) utilized early...
Multidrug resistance (MDR) has been reported in both prokaryotes and eukaryotes, underscoring the challenge of design screening more efficacious new drugs. For instance, efflux pump Pseudomonas aeruginosa (gram-negative bacteria) can extrude a variety structurally functionally diverse substrates, which leads to MDR. In this study, we present platform that studies modes action antibiotics living bacterial cells (P. aeruginosa), real-time, at nanometer scale single-cell resolution using...
We synthesized tiny stable silver nanoparticles (2.6 ± 1.1 nm) and used its small surface area functional groups to control single molecule detection (SMD) volumes on nanoparticles. These new approaches allowed us develop intrinsic nanoparticle optical biosensors (SMNOBS) for sensing imaging of human cytokine molecules, recombinant tumor necrosis factor-α (TNFα), probing binding reaction with monoclonal antibody (MAB) molecules in real-time. found that SMNOBS retained their biological...
We report here the use of a simple washing approach to reduce ionic strength solution, which increased thickness electric double layer on surface silver (Ag) nanoparticles and thereby enhanced their zeta-potential. This allowed us prepare optically uniform (75-99%) purified Ag (11.3 +/- 2.3 nm) that are stable (nonaggregation) in solution for months, permitting them become robust widely used single nanoprobes vivo optical imaging. These show remarkable photostability serve as nanoparticle...
Nanomaterials possess unusually high surface area-to-volume ratios and surface-determined physicochemical properties. It is essential to understand their surface-dependent toxicity in order rationally design biocompatible nanomaterials for a wide variety of applications. In this study, we have functionalized the surfaces silver nanoparticles (Ag NPs, 11.7 ± 2.7 nm diameter) with three peptides (CALNNK, CALNNS, CALNNE) prepare positively (Ag-CALNNK NPs(+ζ)), negatively (Ag-CALNNS NPs(-2ζ)),...
At the cellular level, a small number of protein molecules (receptors) can induce significant responses, emphasizing importance molecular detection trace amounts on single living cells. In this study, we designed and synthesized silver nanoparticle biosensors (AgMMUA-IgG) by functionalizing 11.6 +/- 3.5-nm Ag nanoparticles with mixed monolayer 11-mercaptoundecanoic acid (MUA) 6-mercapto-1-hexanol (1:3 mole ratio) covalently conjugating IgG MUA surface. We found that preserve their biological...
We have synthesized and purified silver nanoparticles (Ag NPs) (11.3 ± 2.3 nm) that are stable (non-aggregated) in cell culture medium inside single living cells. developed new imaging methods to characterize sizes number of NPs the cells real-time determine their stability (non-aggregation) at NP resolution. These approaches allow us study toxic therapeutic effects Ag on tumor (L929, mouse fibroblast cells) with determined concentrations (doses) over time found inhibited growth division...
Diabetes mellitus, especially type 2 diabetes (T2DM), is one of the most common chronic diseases and continues to increase in numbers with large proportion health care budget being used. Many animal models have been established order investigate mechanisms pathophysiologic progress T2DM find effective treatments for its complications. On basis their strains, features, advantages, disadvantages, various types can be divided into spontaneously diabetic models, artificially induced...
Abstract Elevated levels of TGFβ are a negative prognostic indicator for patients diagnosed with pancreatic cancer; as result, the pathway is an attractive target therapy. However, clinical application pharmacologic inhibition remains challenging because has tumor suppressor functions in many epithelial malignancies, including cancer. In fact, direct neutralization promotes progression genetic murine models Here, we report that neutralizing activity receptor 2 using monoclonal antibody (2G8)...
Abstract Overexpression of correctly folded membrane proteins is a fundamental prerequisite for functional and structural studies. One the most commonly used expression systems production Escherichia coli . While misfolded typically aggregate form inclusions bodies, that are addressed to extractable by detergents generally assumed be properly folded. Accordingly, GFP fusion strategy often as fluorescent proxy monitor their folding quality. Here we investigated functionality two different...
This study presents the non-layered α-MnSe nanosheets transferred onto LaMnO 3 films to obtain a multifunction heterostructure. The outstanding characteristics are very useful for next-generation devices.
It is well known that surface plasmon absorption (color) of Au nanoparticles highly depends on its size, shape, and surrounding environment, providing a great opportunity for the development as probe chemical biochemical sensing building block nano-optical devices. Currently, it remains incompletely understood how properties ultimately define optical properties, hindering progress rational design assembly Ru(bpy)32+ possesses rich photochemical electrochemical offering unique to study...
Sizes and dynamics of single membrane transporters on living cells, Pseudomonas aeruginosa, were directly measured showing transported substrates up to 80 nm in diameter, which is one order magnitude larger than the reported exclusion limit outer membrane, over times seconds hours. The uptake efflux depend size concentration substrates, incubation time, mutants. strikingly active by mutants devoid pump proteins was observed, suggesting that trigger assembly a new extrusive system. This...