A5007650182- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Electrochemical Analysis and Applications
- Molecular Junctions and Nanostructures
- MicroRNA in disease regulation
- Electrochemical sensors and biosensors
- Advanced MRI Techniques and Applications
- Advanced Nanomaterials in Catalysis
- RNA and protein synthesis mechanisms
- Melanoma and MAPK Pathways
- Advanced Biosensing Techniques and Applications
- Glycosylation and Glycoproteins Research
- Neuroscience and Neuropharmacology Research
- Advanced Battery Technologies Research
- COVID-19 Clinical Research Studies
- Heat shock proteins research
- Pneumonia and Respiratory Infections
- Nanoplatforms for cancer theranostics
- SARS-CoV-2 and COVID-19 Research
- Mass Spectrometry Techniques and Applications
- Quantum Dots Synthesis And Properties
- Hepatitis B Virus Studies
- Analytical Chemistry and Sensors
Southwest University
2016-2025
Guangxi Medical University
2009-2025
First Affiliated Hospital of GuangXi Medical University
2009-2025
Wuhan Children's Hospital
2016-2024
Huazhong University of Science and Technology
2014-2024
Hunan University of Traditional Chinese Medicine
2017-2024
University of Houston
2024
Guangzhou Medical University
2021-2024
Guilin University of Electronic Technology
2023-2024
Third Affiliated Hospital of Southern Medical University
2022-2024
SARS-CoV-2 Infection in Children In this report, investigators Wuhan, China, describe the spectrum of Covid-19 illness children under age 16 years. Of 1391 assessed and tested...
The coupling of aptamers with the coding and amplification features inorganic nanocrystals is shown for first time to offer a highly sensitive selective simultaneous bioelectronic detection several protein targets. This accomplished in single-step displacement assay connection self-assembled monolayer thiolated conjugated proteins carrying different nanocrystals. Electrochemical stripping nondisplaced nanocrystal tracers results remarkably low (attomole) limit, that is, significantly lower...
This study addresses the role of PTEN loss in intrinsic resistance to BRAF inhibitor PLX4720. Immunohistochemical staining a tissue array covering all stages melanocytic neoplasia (n = 192) revealed expression be lost >10% melanoma cases. Although status did not predict for sensitivity growth inhibitory effects PLX4720, it was predictive apoptosis, with only limited cell death observed melanomas lacking (PTEN-). Mechanistically, PLX4720 found stimulate AKT signaling PTEN- but PTEN+ lines....
In this work, we describe a new universal and highly sensitive strategy for electrochemiluminescent (ECL) detection of sequence specific DNA at the femtomolar level via in situ hybridization chain reaction (HCR) signal amplification. The capture probes are self-assembled on gold electrode. presence target two hairpin helper DNAs leads to formation extended dsDNA polymers through HCR electrode surface. situ, HCR-generated cause intercalation numerous ECL indicators (Ru(phen)(3)(2+)) into...
The epidemiologic and clinical characteristics of pediatric patients with coronavirus disease 2019 (COVID-19) have been reported, but information on immune features associated severity is scarce.To delineate compare the immunologic mild moderate COVID-19 in patients.This single-center case series included 157 admitted to Wuhan Children's Hospital laboratory-confirmed severe acute respiratory syndrome 2 (SARS-CoV-2). Data were collected from January 25 April 18, 2020.Documented SARS-CoV-2...
The clinical use of BRAF inhibitors is being hampered by the acquisition drug resistance. This study shows potential therapeutic HSP90 inhibitor (XL888) in six different models vemurafenib resistance.The ability XL888 to inhibit growth and induce apoptosis tumor regression vemurafenib-resistant melanoma cell lines was shown vitro vivo. A novel mass spectrometry-based pharmacodynamic assay developed measure intratumoral HSP70 levels following inhibition lines, xenografts, biopsies....
A target induced cycling strand displacement amplification (SDA) mediated by phi29 DNA polymerase (phi29) was first investigated and applied in a signal-off electrochemiluminescence (ECL) biosensor for microRNA (miRNA) detection. Herein, the miRNA triggered phi29-mediated SDA which could produce amounts of single-stranded (assistant probe) with accurate comprehensive nucleotide sequence. Then, assistant probe hybridized capture ferrocene-labeled (Fc-probe) to form ternary "Y" structure ECL...
On the basis of use silver nanoclusters (AgNCs) in situ synthesized by cytosine (C)-rich loop DNA templates as signal amplification labels, development a label-free and highly sensitive method for electrochemical detection microRNA (miRNA-199a) is described. The target miRNA-199a hybridizes with partial dsDNA probes to initiate target-assisted polymerization nicking reaction (TAPNR) produce massive intermediate sequences, which can be captured on sensing electrode self-assembled secondary...
Activators of M<sub>1</sub> muscarinic acetylcholine receptors (mAChRs) may provide novel treatments for schizophrenia and Alzheimer9s disease. Unfortunately, the development M<sub>1</sub>-active compounds has resulted in nonselective activation highly related M<sub>2</sub> to M<sub>5</sub> mAChR subtypes, which results dose-limiting side effects. Using a functional screening approach, we identified several ligands that potentiated agonist with low micromolar potencies induced 5-fold or...
We here report on the first example of an aptamer-based potentiometric sandwich assay proteins. The measurements are based CdS quantum dot labels secondary aptamer, which were determined with a novel solid-contact Cd2+-selective polymer membrane electrode after dissolution hydrogen peroxide. exhibited cadmium ion detection limits 100 pM in mL samples and 1 nM 200 μL microwells, using calcium-selective as pseudoreference electrode. As prototype example, thrombin was measured lower limit 0.14...
The detection of microRNA expression profiles plays an important role in early diagnosis different cancers. On the basis employment redox labels with distinct potential positions and duplex specific nuclease (DSN)-assisted target recycling signal amplifications, we have developed a multiplexed convenient electronic sensor for highly sensitive (miRNA)-141 miRNA-21. is constructed by self-assembly thiol-modified, species-labeled hairpin probes on gold sensing electrode. hybridizations between...
An alternative "signal on" immunosensor for ultrasensitive detection of apurinic/apyrimidinic endonuclease 1 (APE-1) was designed utilizing the self-enhanced electrochemiluminescence (ECL) a novel Ru(II) complex functionalized coil-like nanocomposite as signal labels. The desirable ECL luminophore achieved by combining coreactant poly(ethylenimine) (PEI) and luminophor bis(2,2′-bipyridine)-5-amino-1,10-phenanthroline ruthenium(II) [Ru(bpy)2(5-NH2-1,10-phen)2+] to form one complex, which...
Thrombin plays important roles for the diagnosis of neurodegenerative and cardiovascular diseases. By integrating proximity binding-induced strand displacement metal ion-dependent DNAzyme recycling amplification, we demonstrate here development a simple sensitive strategy detection thrombin in human serums. The binding two distinct aptamers to targets increases local concentration facilitates release enzymatic sequences through displacement. liberated further hybridize with G-quadruplex...
Stroke is one of the major causes death and disability in United States. After cerebral ischemia reperfusion injury, generation reactive oxygen species (ROS) nitrogen may contribute to disease process through alterations structure DNA, RNA, proteins, lipids. We generated various nanoparticles (liposomes, polybutylcyanoacrylate (PBCA), or poly(lactide-co-glycolide) (PLGA)) that contained active superoxide dismutase (SOD) enzyme (4,000 20,000 U/kg) mouse model injury determine impact these...
The variations in microRNA (miRNA) expression levels can be useful biomarkers for the diagnosis of different cancers. In this work, on basis a new miRNA-triggered molecular machine enzyme-free target recycling signal amplification, development simple electronic sensor highly sensitive detection miRNA-21 from human breast cancer cells is described. three-stand DNA duplex probes are self-assembled gold electrode surface to fabricate sensor. binds terminal toehold region probes, displaces one...
The development of convenient and sensitive methods without involving any enzymes or complex nanomaterials for the monitoring proteins is great significance in disease diagnostics. In this work, we describe validation a new aptamer/protein proximity binding-triggered molecular machinery amplification strategy electrochemical assay thrombin serum samples. sensing interface prepared by self-assembly three-stranded DNA complexes on gold electrode. association two distinct functional aptamers...
In situ monitoring of hydrogen peroxide (H2O2) secreted from live cells plays a critical role in elucidating many cellular signaling pathways, and it is significant challenge to selectively detect these low levels endogenous H2O2. To address this challenge, we report the establishment trimetallic hybrid nanoflower-decorated MoS2 nanosheet-modified sensor for H2O2 MCF-7 cancer cells. The Au–Pd–Pt nanoflower-dispersed nanosheets are synthesized by simple wet-chemistry method, resulting...
The identification and monitoring of circulating tumor cells (CTCs) in human blood plays a pivotal role the convenient diagnosis different cancers. However, it remains major challenge to monitor these CTCs because their extremely low abundance blood. Here, we describe synthesis new aptamer-functionalized gold nanoparticle (AuNP) array-decorated magnetic graphene nanosheet recognition probe capture isolate rare from whole In addition, by employing aptamer/electroactive species-loaded AuNP...
The monitoring of intracellular microRNAs plays important roles in elucidating the biological function and biogenesis miRNAs living cells. However, because their sequence similarity, low abundance, small size, it is a great challenge to detect miRNAs, especially for those with much lower expression levels. To address this issue, we have developed an cell signal amplification approach down-regulated cells based on biodegradable MnO2 nanosheet-mediated target-triggered assembly hairpins....