A5026355928- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- MicroRNA in disease regulation
- Electrochemical Analysis and Applications
- Molecular Junctions and Nanostructures
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
- Advanced Nanomaterials in Catalysis
- DNA Repair Mechanisms
- Molecular Sensors and Ion Detection
- Nanopore and Nanochannel Transport Studies
- Melamine detection and toxicity
- Monoclonal and Polyclonal Antibodies Research
- Epigenetics and DNA Methylation
- Nanocluster Synthesis and Applications
- Advanced Chemical Sensor Technologies
- Electrochemical sensors and biosensors
- Click Chemistry and Applications
- Advanced Biosensing Techniques and Applications
- Machine Learning in Bioinformatics
- Cell Adhesion Molecules Research
- Congenital Ear and Nasal Anomalies
Chongqing University of Technology
2022-2025
Southwest University
2015-2020
Chongqing University
2015-2019
The monitoring of intracellular microRNAs plays important roles in elucidating the biological function and biogenesis miRNAs living cells. However, because their sequence similarity, low abundance, small size, it is a great challenge to detect miRNAs, especially for those with much lower expression levels. To address this issue, we have developed an cell signal amplification approach down-regulated cells based on biodegradable MnO2 nanosheet-mediated target-triggered assembly hairpins....
The detection of specific intracellular microRNAs (miRNAs) in living cells can potentially provide insight into the causal mechanism cancer metastasis and invasion. However, because characteristic nature miRNAs terms small sizes, low abundance, similarity among family members, it is a great challenge to monitor cells, especially those with much lower expression levels. In this work, we describe establishment DNA-fueled catalytic molecule machinery cell signal amplification approach for...
Despite the widespread utilization of gold nanoparticles and graphene for in vivo applications, complex steps preparation functionalization these nanomaterials are commonly required. In addition, cytotoxicity such materials is currently still under debate. this work, by taking significant advantages DNA terms biocompatibility, nontoxicity, controllability as building blocks nanostructures, we describe construction a reconfigurable, multicolor-encoded nanostructure multiplexed monitoring...
MicroRNAs (miRNAs) are useful biomarkers for the diagnosis of a variety cancers. However, it is major challenge to detect miRNAs, considering their high sequence similarity, low concentration, and small size nature. With establishment an efficient rolling circle amplification (RCA) molecular network by target-driven polymerization/nicking reactions, we present here exponential strategy detecting miRNA in label-free way with ultrahigh sensitivity. The target sequences can bind two ssDNA...
A DNA-fueled molecular machine enables non-enzymatic target recycling amplification detection of microRNA from human prostate cancer cells.
Target-triggered assembly of bio-cleavable nanoprobes leads to the highly sensitive imaging intracellular microRNA-21 in live cancer cells.
Self-assembled functional DNA structures have proven to be excellent materials for designing and implementing a variety of nanoscale devices. We demonstrate here that rationally designed programmable ring/hairpin-constrained structure can achieve in situ ligation-free rolling circle amplification (RCA), which further leads highly specific, sensitive, multicolor imaging mRNA molecules single cells. Such aims at addressing current challenges terms simplicity, sensitivity, multiplexing...
A novel inter-particle DNA walker amplification coupled with target self-convert for sensitive detection of miRNAs by liquid chromatography has been proposed.
Simultaneous detection of the concentration variations microRNA-221 (miRNA-221) and PTEN mRNA molecules in PI3K/AKT signaling pathway is significance to elucidate cancer cell migration invasion, which useful for diagnosis therapy. In this work, we show biodegradable MnO2 nanosheet-assisted target-triggered DNAzyme recycling signal amplification cascaded approach specific live cells via simultaneous sensitive monitoring variation intracellular miRNA-221 mRNA. Our nanoprobes enable highly...
Target-induced assembly formation of netlike DNA nanostructures leads to highly sensitive detection cytokines.
Cu<sup>+</sup>-mediated click ligation of DNA leads to catalytic self-assembly hairpins and amplified fluorescent signals for sensitive detection Cu<sup>2+</sup>.
A protein-based therapy is significantly challenged by the successful delivery of native proteins into targeted cancer cells. We address this challenge here using an all-sealed divalent aptamer tetrahedral DNA framework (asdTDF) platform, in which protein drug encapsulated inside cavity stoichiometrically via a reversible chemical bond. The ligase-assisted seal nicks results highly enhanced TDF stability against nuclease digestion to effectively protect therapeutic from degradation. In...