A new signal amplification strategy based on thionine (TH)-doped magnetic gold nanospheres as labels and horseradish peroxidase (HRP) enhancer holds promise to improve the sensitivity detection limit of immunoassay for carcinoembryonic antigen (CEA), a model protein. This system was fabricated carbon fiber microelectrode (CFME) covered with well-ordered anti-CEA/protein A/nanogold architecture. The reverse micelle method initially used preparation TH-doped (nanospheres), synthesized were...

In this work, we describe a new universal and highly sensitive strategy for electrochemiluminescent (ECL) detection of sequence specific DNA at the femtomolar level via in situ hybridization chain reaction (HCR) signal amplification. The capture probes are self-assembled on gold electrode. presence target two hairpin helper DNAs leads to formation extended dsDNA polymers through HCR electrode surface. situ, HCR-generated cause intercalation numerous ECL indicators (Ru(phen)(3)(2+)) into...

Herein, by directly using Watson-Crick base pairing, a highly ordered and field-free three-dimensional (3D) DNA nanostructure is self-assembled azobenzene (azo)-functionalized nippers in few minutes, which was applied as 3D nanomachine with an improved movement efficiency compared to traditional Au-based nanomachines due the organized high local concentration of on homogeneous nanostructure. Once microRNA (miRNA) interacts nanomachine, "open" hybridize miRNA. Impressively, photoisomerization...

On the basis of a novel target-cycling synchronized rolling circle amplification (RCA) as signal strategy and in situ electrochemical generation silver nanoclusters (Ag NCs) probes, an ultrasensitive simple electrochemiluminescence (ECL) biosensor was proposed for microRNA (miRNA) detection. It worth mentioning that circular template subtly designed to consist guanine-rich (G-rich) region binding realizing RCA. In presence target miR-21, hybridized with primer miR-21 form ternary "P"...

In this study, an off-on switching of a dual amplified electrochemiluminescence (ECL) biosensor based on Pb(2+)-induced DNAzyme-assisted target recycling and rolling circle amplification (RCA) was constructed for microRNA (miRNA) detection. First, the primer probe with assistant miRNA formed Y junction which cleaved addition Pb(2+) to release miRNA. Subsequently, released could initiate next process, leading generation numerous intermediate DNA sequences (S2). Afterward, bare glassy carbon...

Herein, we observed near-infrared electrochemiluminescence (NIR ECL) emission from tetraphenylethylene nanocrystals (TPE NCs), which exhibit high ECL efficiency and excellent biocompatibility compared with the current NIR emitters (such as semiconductor quantum dots metal nanoclusters). The strong signal of TPE NCs originates aggregation-induced enhanced via improvement electron hole recombination suppression nonradiative transition. Impressively, an enormous red-shifted (678 nm) relative to...

The development of a sensitive and practical electrochemiluminescence (ECL) bioassay relies on the use ECL signal tags whose intensity is high stable. In this work, strong emission was achieved from metal organic framework (MOF) accelerator enriched quantum dots (CdTe), which were applied as an efficient tag for trace biomarker detection. It particularly noteworthy that novel mechanism to drastically enhance CdTe established because isoreticular framework-3 (IRMOF-3) with 2-amino...

We report for the first time phenomenon of continuously color-tunable ECL from individual Au NCs confined in a porous hydrogel matrix. The property arises dynamic surface reconstruction and co-reactants.

This study demonstrates a novel approach toward development of advanced immunosensors based on chemically functionalized core−shell Fe3O4@Ag magnetic nanoparticles, and the preparation, characterization, measurement relevant properties immunosensor useful for detection carcinoembryonic antigen (CEA) in clinical immunoassay. The combination nanocore an Ag metallic shell shows good adsorption attachment CEA antibody selective to CEA. nanostructure presents facilitate modulate way it was...

In this work, we developed a sensitive and efficient ratiometric electrochemical method for lipopolysaccharide (LPS) detection using Cu-based metal-organic frameworks (Cu-MOFs) as catalyst target-triggered quadratic cycles signal amplification. First, in the presence of target LPS, conformation change specifically designed hairpin probes 1 (HP1) triggered cyclic-induced polymerization to produce output DNA with aid phi29 polymerase (phi29). Then, obtained hybridized ferrocene-labeled 2...

A new type of multifunctional metal–organic framework (MOF) has been synthesized by encapsulating hemin into the nano-sized Fe-MIL-88 MOFs (hemin@MOFs) and first applied in an electrochemical aptasensor to detect thrombin (TB) with aid enzyme for signal amplification. The gold nanoparticle functionalized hemin@MOFs (Au/hemin@MOFs) have not only simultaneously served as redox mediators solid electrocatalysts, but also utilized ideal loading platform immobilize a large number biomolecules. In...

A target induced cycling strand displacement amplification (SDA) mediated by phi29 DNA polymerase (phi29) was first investigated and applied in a signal-off electrochemiluminescence (ECL) biosensor for microRNA (miRNA) detection. Herein, the miRNA triggered phi29-mediated SDA which could produce amounts of single-stranded (assistant probe) with accurate comprehensive nucleotide sequence. Then, assistant probe hybridized capture ferrocene-labeled (Fc-probe) to form ternary "Y" structure ECL...

In this paper, a novel electrochemiluminescence resonance energy transfer (ECL-RET) system from O2/S2O82– to kind of amino-terminated perylene derivative (PTC-NH2) was demonstrated for the first time, which then applied construct ratiometric aptasensor lead ion (Pb2+) detection. First, gold-nanoparticles-functionalized fullerene nanocomposites (AuNPs@nano-C60) were coated on glassy carbon electrode (GCE), and thiol-modified assistant probes (APs) attached AuNPs@nano-C60/GCE. Then resultant...

On the basis of use silver nanoclusters (AgNCs) in situ synthesized by cytosine (C)-rich loop DNA templates as signal amplification labels, development a label-free and highly sensitive method for electrochemical detection microRNA (miRNA-199a) is described. The target miRNA-199a hybridizes with partial dsDNA probes to initiate target-assisted polymerization nicking reaction (TAPNR) produce massive intermediate sequences, which can be captured on sensing electrode self-assembled secondary...

In this work, ceria doped ZnO nanomaterials with flower-structure (Ce:ZONFs) were prepared to construct a luminol-based electrochemiluminescence (ECL) immunosensor for amyloid-β protein (Aβ) detection. Herein, carboxyl groups (−COOH) covered Ce:ZONFs synthesized by green method lysine as reductant. After that, Ce:ZONFs-based ECL nanocomposite was combining the luminophore of luminol and via amidation physical absorption. Luminol modified on surface could generate strong signal under...

A highly sensitive electrochemiluminescent (ECL) aptasensor was constructed using semicarbazide (Sem) as co-reaction accelerator to promote the ECL reaction rate of CdTe quantum dots (CdTe QDs) and co-reactant peroxydisulfate (S2O82–) for boosting signal amplification. The is a species that when it introduced into system containing luminophore co-reactant, can interact with rather than co-reactant; thus significantly amplified in comparison which only are present. In this work, probes were...

The detection of microRNA expression profiles plays an important role in early diagnosis different cancers. On the basis employment redox labels with distinct potential positions and duplex specific nuclease (DSN)-assisted target recycling signal amplifications, we have developed a multiplexed convenient electronic sensor for highly sensitive (miRNA)-141 miRNA-21. is constructed by self-assembly thiol-modified, species-labeled hairpin probes on gold sensing electrode. hybridizations between...