A5110035798- Neuroinflammation and Neurodegeneration Mechanisms
- Anesthesia and Neurotoxicity Research
- Intensive Care Unit Cognitive Disorders
- Cancer, Stress, Anesthesia, and Immune Response
- Neurological Disease Mechanisms and Treatments
- Mast cells and histamine
- Immune Response and Inflammation
- Olfactory and Sensory Function Studies
- Cancer, Hypoxia, and Metabolism
- Pain Mechanisms and Treatments
- Neuroscience and Neuropharmacology Research
- Natural product bioactivities and synthesis
- Neuropeptides and Animal Physiology
- Neuroscience of respiration and sleep
- Neuroendocrine regulation and behavior
- melanin and skin pigmentation
- Tryptophan and brain disorders
- Alzheimer's disease research and treatments
- Infant Health and Development
- Barrier Structure and Function Studies
- Anesthesia and Sedative Agents
- S100 Proteins and Annexins
- Cardiac, Anesthesia and Surgical Outcomes
- Pharmacological Effects of Natural Compounds
- Extracellular vesicles in disease
Chongqing Medical University
2025
Jiangsu Province Hospital
2011-2022
Nanjing Medical University
2012-2022
Jiangsu Provincial Hospital of Traditional Chinese Medicine
2012-2013
National Institutes of Health
2001
National Institute of Mental Health
2001
Neuroinflammation plays a key role in the occurrence and development of postoperative cognitive dysfunction (POCD). Microglia, resident immune cells brain, has been increasingly recognized to contribute neuroinflammation. Although brain mast (MCs) are "first responder" injury rather than microglia, little is known about functional aspects MCs-microglia interactions. Male Sprague-Dawley (SD) rats were injected intracerebroventricular with MC stabilizer Cromolyn (100 μg/μl), stimulator C48/80...
Neuroinflammation is considered a risk factor for impairments in neuronal function and cognition that arise with trauma, infection, and/or disease. IL-17A has been determined to be involved neurodegenerative diseases such as multiple sclerosis. Recently, shown upregulated lipopolysaccharide(LPS)-induced systemic inflammation. This study aims explore the role of LPS-induced neuroinflammation cognitive impairment.Male Sprague-Dawley (SD) rats were injected intraperitoneally LPS (500 μg/kg),...
Lithium, an effective mood stabilizer for the treatment of bipolar disorders, has been recently suggested to have a role in neuroprotection during neurodegenerative diseases. The pathogenesis neurological disorders often involves activation microglia and associated inflammatory processes. Thus, this study, we aimed understand lithium microglial elucidate underlying mechanism(s).Primary cells were pretreated with stimulated lipopolysaccharide (LPS). assessed regarding responses...
The development of antinociceptive tolerance following repetitive administration opioid analgesics significantly hinders their clinical use. Evidence has accumulated indicating that microglia within the spinal cord plays a critical role in morphine tolerance. inhibitor is effective to attenuate tolerance; however, mechanism not fully understood. Our present study investigated effects and possible natural product procyanidins improving via its specific inhibition on NOD-like receptor protein3...
Abstract Background Neuroinflammation, which ultimately leads to neuronal loss, is considered play a crucial role in numerous neurodegenerative diseases. The neuroinflammatory process characterized by the activation of glial cells such as microglia. Endoplasmic reticulum (ER) stress commonly associated with impairments function and cognition, but its relationship neurodegeneration still controversial. Recently, it was confirmed that nonharmful levels ER protected against experimental...
Tolerance seriously impedes the application of morphine in clinical medicine. Thus, it is necessary to investigate exact mechanisms and efficient treatment. Microglial activation neuroinflammation spinal cord are thought play pivotal roles on genesis maintaining tolerance. Activation adenosine monophosphate-activated kinase (AMPK) has been associated with inhibition inflammatory nociception. Metformin, a biguanide class antidiabetic drugs activator AMPK, potential anti-inflammatory effect....
Background/Aims: Microglia are an essential player in central nervous system inflammation. Recent studies have demonstrated that the astrocytic chemokine, CCL2, is associated with microglial activation vivo. However, CCL2-induced has not yet been studied vitro. The purpose of current study was to understand role astrocyte-derived CCL2 and elucidate underlying mechanism(s). Methods: Primary astrocytes were pre-treated siRNA stimulated TNF-α. culture medium (CM) collected added cultures...
Background The functional aspects of mast cell-microglia interactions are important in neuroinflammation. We have previously found that cell degranulation could directly induce microglia activation. However, the role LPS-induced activation, neuroinflammation and cognitive impairment has not been clarified. Methods studied brain cells interaction by site-directed injection cromolyn rat right hypothalamus using stereotaxic techniques. TFC Y maze were used to assess function. Mast stained...
Background/Aims: Astrocytes are critical for the development of postoperative cognitive dysfunction (POCD). In addition, astrocytes express toll-like receptors 4 (TLR4) and build up responses to innate immune triggers by releasing pro-inflammatory molecules. The pathogenesis neurological disorders often involves activation associated inflammatory processes. Lithium, a primary drug treatment bipolar disorder, has recently been suggested have role in neuroprotection during neurodegenerative...
Astrocytes have attracted increasing attention over recent decades for their role in neuroinflammation. Histamine, a major aminergic brain neurotransmitter, has an important influence on the main activities of astrocytes, such as ion homeostasis, energy metabolism, and neurotransmitter clearance. However, little is known about impact histamine astrocyte immunomodulatory function.The expression all receptor subtypes was examined primary astrocytes. Then, astrocytes were pretreated with...
Metastasis is a major cause of prostate cancer (PCa)-related deaths in men. Recent studies have indicated that VGF nerve growth factor inducible (VGF) affects tumor invasion and metastasis. The present study investigated whether abnormally expressed PCa progression the specific regulatory mechanisms by which sh- hypoxia-inducible factor1 alpha (HIF-1α) plasmid was transfected into human cell lines 22Rv1 C4-2 to create with stable low expression overexpression VGF. Quantitative PCR (qPCR)...
Astrocytes are proving to be critical for the development of cognitive functions. In addition, astrocytic activation contributes impairment induced by chronic cerebral hypoperfusion. Minocycline has been shown exhibit long-term neuroprotective effects in vascular rat models through inhibition astrogliosis, and demonstrated potential prevention treatment postoperative decline elderly patients. This study aimed examine effect minocycline on hippocampal astrocytes dysfunction aged mice. Mice...
Morphine tolerance is a clinical challenge, and its pathogenesis closely related to the neuroinflammation mediated by Toll-like receptor 4 (TLR4). In Chinese pain clinic, lidocaine combined with morphine treat chronic pain. We found that sufficiently inhibited induced improved analgesic on basis of non-affecting threshold.CD-1 mice were utilized for tail-flick test evaluate tolerance. The microglial cell line BV-2 was investigate mechanism lidocaine. Neuroinflammation-related cytokines...
Abstract Objective Neuroinflammation plays a critical role in central nervous system diseases. Exosomal miRNAs released from various cells are implicated cell-to-cell communication. Prior studies have placed substantial emphasis on the of cytokines mast cell-microglia interactions during neuroinflammation. However, it has never been clearly determined whether exosomal participate interaction between and microglia thus mediate Methods The characteristics exosomes isolated cell culture...
Background: The pathophysiological mechanisms underlying postoperative cognitive dysfunction (POCD) remain unclear over the years. Neuroinflammation caused by surgery has been recognized as an important element in development of POCD. Many studies also suggest that vagus nerve plays role transmitting peripheral injury signals to central nervous system (CNS) and resultant neuroinflammation. Previously, we have demonstrated brain mast cells (BMCs), "first responders", play a vital...
Mast cells (MCs), the 'first responders' in brain injury, are able to disrupt blood–brain barrier (BBB), but underlying mechanism is not well understood. Tryptase most abundant MC secretory product. Protease-activated receptor 2 (PAR-2) has been identified as a specific for tryptase, which abundantly expressed microvascular endothelial cells. The BBB comprises that display specialised molecular properties essential function and integrity. Therefore, purpose of present study was investigate...