A5079380952- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Graphene and Nanomaterials Applications
- Nanoparticles: synthesis and applications
- RNA Interference and Gene Delivery
- Bone Tissue Engineering Materials
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- CAR-T cell therapy research
- Extracellular vesicles in disease
- Cancer Immunotherapy and Biomarkers
- Carbon and Quantum Dots Applications
- Cancer-related molecular mechanisms research
- Autophagy in Disease and Therapy
- Cancer, Lipids, and Metabolism
- Computational Drug Discovery Methods
- Erythrocyte Function and Pathophysiology
- Kruppel-like factors research
- RNA modifications and cancer
- T-cell and B-cell Immunology
- MXene and MAX Phase Materials
- Cell Image Analysis Techniques
- Gastric Cancer Management and Outcomes
- Hemoglobin structure and function
- Cancer, Hypoxia, and Metabolism
University of Pennsylvania
2021-2025
Chongqing Medical University
2025
Guangdong Province Women and Children Hospital
2023-2025
Jiangmen Central Hospital
2025
Southern Medical University
2015-2024
Guangzhou Medical University
2023-2024
Stomatology Hospital
2024
First Affiliated Hospital of Jinan University
2016-2023
First Affiliated Hospital of University of South China
2019
University of South China
2019
BackgroundOnly a minority of melanoma patients experience durable responses to immunotherapies due inter- and intra-tumoral heterogeneity in melanoma. As result, there is pressing need for suitable preclinical models investigate resistance mechanisms enhance treatment efficacy.MethodsHere, we report two different methods generating patient-derived organoids (MPDOs), one embedded collagen gel, the other inlaid Matrigel. MPDOs Matrigel are used assessing therapeutic effects anti-PD-1...
Evidence indicates that microRNAs (miRNAs) play vital roles in regulating osteogenic differentiation and bone formation. Methods: Here, we show a polyethyleneimine (PEI)-functionalized graphene oxide (GO) complex efficiently loaded with the miR-214 inhibitor is assembled into silk fibroin/hydroxyapatite (SF/HAP) scaffolds spatially control release of inhibitor. Results: SF/HAP/GO nanosized GO high mechanical strength, their hierarchical microporous structures promote cell adhesion growth....
Regulatory T cells (Tregs) are essential in the maintenance of immunity, and they also a key to immune suppressive microenvironment solid tumors. Many studies have revealed biology Tregs various human pathologies. Here we review recent understandings immunophenotypes functions melanoma, including Treg recruitment expansion tumor. frequently accumulated melanoma ratio CD8+ versus is predictive for patient survival. Hence, depletion promising strategy enhancement anti-melanoma immunity. aimed...
Small extracellular vesicles (sEVs) are nanosized vesicles. Death receptor 5 (DR5) mediates extrinsic apoptosis. We engineer DR5 agonistic single-chain variable fragment (scFv) expression on the surface of sEVs derived from natural killer cells. PDGFR transmembrane domain delivers DR5-scFvs to sEVs. DR5-scFv rapidly induce apoptosis different types + cancer cells, myeloid-derived suppressor cells (MDSCs), and cancer-associated fibroblasts (CAFs). migrate specifically tumors in vitro vivo....
Metastasis is a major cause of prostate cancer (PCa)-related deaths in men. Recent studies have indicated that VGF nerve growth factor inducible (VGF) affects tumor invasion and metastasis. The present study investigated whether abnormally expressed PCa progression the specific regulatory mechanisms by which sh- hypoxia-inducible factor1 alpha (HIF-1α) plasmid was transfected into human cell lines 22Rv1 C4-2 to create with stable low expression overexpression VGF. Quantitative PCR (qPCR)...
To evaluate the 5-year survival rates of patients with early cervical cancer (CC) under different surgical approaches and to analyze factors affecting prognosis these patients. A retrospective analysis follow-up study were conducted on who underwent treatment for CC at Guangdong Women Children Hospital between January 2005 December 2017. Prognostic analyzed using Kaplan–Meier method Cox regression model. total 726 included, 347 in open surgery group 379 laparoscopy group. The proportion deep...
Progression to castration resistance is the leading cause of death in prostate cancer patients. Long non-coding RNAs (lncRNAs) have recently become a focal point regulation development. However, few lncRNAs associated with castration-resistant (CRPC) been reported. Firstly, we explore CRPC by RNA sequencing and validated using quantitative polymerase chain reaction (qRT-PCR) fluorescence situ hybridization (RNA-FISH). The clinical significance FLJ was evaluated collected cohort. Functional...
To explore whether nanoparticles (NPs) can be transported into the CNS via taste nerve pathway.ZnO and TiO2 NPs were tongue-instilled to male Wistar rats. Toxicity was assessed by Zn/Ti biodistribution, histopathological examination, oxidative stress assay, quantitative reverse-transcriptase PCR analysis, learning memory capabilities.ZnO significantly deposited in nerves brain, respectively. The examination indicated a slight injury cerebral cortex hippocampus. Ultrastructural changes an...
Abstract Diabetes and periodontal diseases have a mutual promoting relationship that induces severe tissue damage cell death. The potential roles of microRNAs (miRNAs) the type death involved in diabetes‐associated periodontitis are obscure. gingival tissues patients were obtained MC3T3‐E1 cells costimulated with high glucose lipopolysaccharide (LPS). Osseous morphometric analysis was evaluated micro‐CT, histological characteristics measured by hematoxylin/eosin immunohistochemical staining....
Fibroblast growth factor receptors (FGFRs) have been implicated in the malignant transformation and chemoresistance of epithelial ovarian cancer; however, underlying molecular mechanisms are poorly understood. Increased sialyltransferase activity that enhances protein sialylation is an important post‑translational process promoting cancer progression malignancy. In present study, α2,6‑sialyltransferase (ST6Gal‑I) overexpression or knockdown cell lines were developed, FGFR1 was examined to...
The importance of immunosuppressive myeloid-derived suppressor cells (MDSCs) bearing monocyte markers in tumor metastasis has been well established. Recently, it was reported that these possess phenotypic plasticity and differentiate into fibrocytes, very distinct are precursors tumorigenic myofibroblasts. However, the this transdifferentiation not explored. Here, we describe role MDSC-derived fibrocytes is regulated by Kruppel-like factor 4 (KLF4), a transcription critical to...
MicroRNAs (miRNAs) are widely believed to be promising targets for oral squamous cell carcinoma (OSCC) gene therapy. miR-214 has been identified as a promoter of OSCC aggression and metastasis.Graphene oxide-polyethylenimine (GO-PEI) complexes were prepared loaded with miRNA inhibitor at different N/P ratios. The transfection efficiency GO-PEI-inhibitor was tested in Cal27 SCC9 cells. Moreover, the tumor inhibition ability measured an xenograft mouse model by intratumoral injection.Here, we...
Background Gamma delta (γδ) T cells are attractive effector for cancer immunotherapy. Vδ2 expanded by zoledronic acid (ZOL) the most commonly used γδ adoptive cell therapy. However, transfer of has limited clinical efficacy. Methods We developed a costimulation method expansion in PBMCs activating T-cell receptor (γδTCR) and Toll-like (TLR) 7/8 using isopentenyl pyrophosphate (IPP) resiquimod, respectively, tested functional markers antitumoral effects vitro two-dimensional three-dimensional...
Atherosclerosis is a chronic inflammatory vascular disease characterized by lipid accumulation and endothelial dysfunction. Cytoglobin has been shown to exert protective effects under oxidative stress conditions. The aim of this study was determine whether recombinant human cytoglobin (rhCYGB) against atherosclerosis. We intraperitoneally injected rhCYGB (0, 4, or 7 mg/kg BW) into the atherosclerotic rats daily for 60 days. injections reduced low-density lipoprotein cholesterol (LDL-C)...
Background Gamma-delta (γδ) T lymphocytes are primed to potently respond pathogens and transformed cells by recognizing a broad range of antigens. However, adoptive immunotherapy with γδT has exhibited mixed treatment responses. Better understanding cell biology stratifying healthy donors for allogeneic therapy is clinically needed fully realize the therapeutic potential cells. Methods We examine 98 blood samples from measure their expansion capacity after zoledronate stimulation, test...
Background: Gene therapy is considered a novel way to treat osteosarcoma, and microRNAs are potential therapeutic targets for osteosarcoma. miR-214 has been found promote osteosarcoma aggression metastasis. Graphene oxide (GO) widely used gene delivery the distinct physiochemical properties minimal cytotoxicity. Methods: Polyethyleneimine (PEI)-functionalized GO complex was well-prepared loaded with inhibitor at different concentrations. The load efficacy tested by gel retardation assay...
Introduction: Cervical cancer (CC) is the fourth most common malignant tumor in term of incidence and mortality among women worldwide. The tricarboxylic acid (TCA) cycle an important hub energy metabolism, networking one-carbon fatty acyl metabolism glycolysis. It can be seen that reprogramming cell including TCA plays indispensable role tumorigenesis development. We aimed to identify genes related as prognostic markers CC. Methods: Firstly, we performed differential expressed analysis gene...