A5082515760- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- Pneumonia and Respiratory Infections
- Viral Infections and Immunology Research
- Escherichia coli research studies
- COVID-19 diagnosis using AI
- Antibiotic Resistance in Bacteria
- Liver Disease Diagnosis and Treatment
- Gut microbiota and health
- Long-Term Effects of COVID-19
- RNA modifications and cancer
- Dental Research and COVID-19
- Genomics and Phylogenetic Studies
- interferon and immune responses
- Hepatitis Viruses Studies and Epidemiology
- Bacterial Identification and Susceptibility Testing
- Vibrio bacteria research studies
- Salmonella and Campylobacter epidemiology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Zhejiang University
2016-2025
State Key Laboratory of Diagnosis and Treatment of Infectious Diseases
2014-2025
First Affiliated Hospital Zhejiang University
2019-2025
National Clinical Research
2020-2023
University of Virginia
2023
Zhejiang Shuren University
2020
Zhejiang Medicine (China)
2020
Chinese Center For Disease Control and Prevention
2013
Wenzhou Medical University
2011
To evaluate viral loads at different stages of disease progression in patients infected with the 2019 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) during first four months epidemic Zhejiang province, China.
Background Timely diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is a prerequisite for treatment and prevention. The serology characteristics complement value the antibody test to RNA need be demonstrated. Method Serial sera 80 patients with PCR-confirmed disease 2019 (COVID-19) were collected at First Affiliated Hospital Zhejiang University, Hangzhou, China. Total (Ab), IgM IgG antibodies against SARS-CoV-2 detected, dynamics during described. Results...
Abstract A woman with coronavirus disease in her 35th week of pregnancy delivered an infant by cesarean section a negative-pressure operating room. The was negative for severe acute respiratory 2. This case suggests that mother-to-child transmission is unlikely this virus.
The coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome 2 (SARS-CoV-2) was identified in December and has subsequently spread worldwide. Currently, there is no effective method to cure COVID-19. Mesenchymal stromal cells (MSCs) may be able effectively treat COVID-19, especially for critical patients. Menstrual blood-derived MSCs have recently received much attention due their superior proliferation ability lack of ethical problems. Forty-four patients were...
The genetic predisposition to severe A(H1N1)2009 (A[H1N1]pdm09) influenza was evaluated in 409 patients, including 162 cases with infection and 247 controls mild infection. We prioritized candidate variants based on the result of a pilot genome-wide association study lung expression quantitative trait locus data set. GG genotype rs2070788, higher-expression variant TMPRSS2, risk (odds ratio, 2.11; 95% confidence interval, 1.18–3.77; P = .01) A(H1N1)pdm09 influenza. A potentially functional...
Background. Systematic analysis of histopathological and serial virological changes fatal influenza A(H7N9) cases is lacking. Methods. Patients with infection admitted to our intensive care unit during 10–23 April 2013 were included. Viral loads in the respiratory tract, as inferred from cycle threshold (Ct) value reverse transcription polymerase chain reaction (RT-PCR), serum hemagglutination inhibition (HAI) antibody titer, analyzed. Postmortem biopsies lung, liver, kidney, spleen, bone...
Abstract The novel avian origin influenza A (H7N9) virus has caused severe diseases in humans eastern China since the spring of 2013. Fatal outcomes H7N9 infections are often attributed to pneumonia and acute respiratory distress syndrome (ARDS). There is urgent need discover biomarkers predicting progression disease fatal outcome potentially lethal flu infections, based on sound statistical analysis. We discovered that 34 48 cytokines chemokines examined this study were significantly...
Serological surveillance conducted in areas of an outbreak influenza A(H7N9) infection China found no seropositivity for antibodies specific avian-origin among 1129 individuals the general population, whereas >6% 396 poultry workers were positive (on basis a hemagglutination inhibition titer ≥ 80) this subtype, confirming that infected is principal source human infections and subclinical are possible. Fourteen days after symptom onset, elevated levels to 65.8% patients (25/38) who survived...
Abstract Background Timely diagnosis of SARS-CoV-2 infection is the prerequisite for treatment and preventive quarantine. The serology characteristics complement value antibody test to RNA needs be demonstrated. Method A patient cohort study was conducted at first affiliated hospital Zhejiang University, China. Serial plasma COVID-19 patients were collected total (Ab), IgM IgG against detected. dynamics during described. Results seroconversion rate Ab, in 98.8% (79/80), 93.8% (75/80)...
Abstract A novel coronavirus (2019-nCoV) was first identified in Wuhan, Hubei Province, and then spreads to the other Provinces of China. WHO decides determine a Public Health Emergency International Concern (PHEIC) 2019-nCoV. 2019-nCov reported share same receptor, Angiotensin-converting enzyme 2 (ACE2), with SARS-Cov. Here based on public single-cell RNA-Seq datasets, we analyzed ACE2 RNA expression profile tissues at different locations respiratory tract. The result indicates that appears...
Coronavirus disease 2019 (COVID-19) broke out and then became a global epidemic at the end of 2019. With increasing number deaths, early identification severity interpretation pathogenesis are very important. Aiming to identify biomarkers for progression COVID-19, 75 COVID-19 patients, 34 healthy controls 23 patients with pandemic influenza A(H1N1) were recruited in this study. Using liquid chip technology, 48 cytokines chemokines examined, among which 33 significantly elevated compared...
Plasma metagenomic next-generation sequencing (mNGS) testing is a promising diagnostic modality for infectious diseases, but its real-world clinical impact poorly understood. We reviewed patients who had undergone plasma mNGS at general hospital to evaluate the utility of testing. A total 76.9% (113/147) tests positive result. 196 microorganisms (58) were identified and reported, which 75.6% (148/196) clinically relevant. The median stringent mapped read number (SMRN) relevant organisms was...
ObjectiveThe risk factors and the impact of NAI treatments in patients with severe influenza A-associated pneumonia remain unclear.MethodsA multicenter, retrospective, observational study was conducted Zhejiang, China during a epidemic August 2017–May 2018. Clinical records (>14 y) hospitalized laboratory-confirmed A virus infection who developed were compared to those mild-to-moderate pneumonia. Risk related severity effects (monotherapy combination peramivir oseltamivir)...
Abstract Background The emergence and rapid spread of the deadly novel coronavirus disease caused by severe acute respiratory syndrome 2 (SARS‐CoV‐2) is a swiftly evolving public health crisis worldwide. SARS‐CoV‐2 infection characterized development progression inflammatory responses. Hematological parameters, such as white blood cells (WBCs) their subpopulations, red cell distribution width, platelet count, mean volume, plateletcrit, derived markers neutrophil‐to‐lymphocyte ratio (NLR),...
Microbial communities such as those residing in the human gut are highly diverse and complex, many with important implications for health diseases. The effects functions of these microbial determined not only by their species compositions diversities but also dynamic intra- inter-cellular states at transcriptional level. Powerful scalable technologies capable acquiring single-microbe-resolution RNA sequencing information order to achieve a comprehensive understanding complex together hosts...
The viral RNA shedding time (VST) for severe acute respiratory syndrome coronavirus 2 has not been well characterized. Clinical data were collected and compared between patients with short long VSTs (in the lower upper quartiles, respectively). probability of recurrent positive reverse-transcription polymerase chain reaction results decreased sharply to 4.8% after 3 consecutive negative results. A series ≥3 was suitable as a criterion end shedding. VST from tract significantly shorter in...
BackgroundNanopore sequencing, known for real-time analysis, shows promise rapid clinical infection diagnosis but lacks effective assays bloodstream infections (BSIs).MethodsWe prospectively assessed the performance of a novel nanopore targeted sequencing (NTS) assay in identifying pathogens and predicting antibiotic resistance BSIs, analyzing 387 blood samples from December 2021 to April 2023.ResultsThe positivity rate NTS (69.5%, 269/387) nearly matches that metagenomic next-generation...
A multiplex real-time PCR assay was developed to simultaneously detect and discriminate influenza virus subtypes, including novel H1N1 (2009) seasonal H3N2 virus, B respiratory syncytial (RSV) in a single test tube, with detection sensitivity specificity of 99% 100%, respectively, for the four pathogens.
Background Plesiomonas shigelloides can cause gastroenteritis and extra-intestinal diseases in humans. However, the prevalence of P. infections has not been investigated China. Methods Consecutive fecal specimens from outpatients with acute diarrhea non-diarrheal patients at nine sentinel hospitals southeast China were collected March 2010 to May 2012. Bacterial pathogens detected by culture, isolates subjected antimicrobial susceptibility testing. We also retrospectively reviewed hospital...