A5026519979- Viral gastroenteritis research and epidemiology
- Salmonella and Campylobacter epidemiology
- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Bacterial Identification and Susceptibility Testing
- Ideological and Political Education
- Escherichia coli research studies
- Hemoglobin structure and function
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- EFL/ESL Teaching and Learning
- Viral Infections and Immunology Research
- Bacteriophages and microbial interactions
- Educational Reforms and Innovations
- Endoplasmic Reticulum Stress and Disease
- Clostridium difficile and Clostridium perfringens research
- Porphyrin Metabolism and Disorders
- Urinary Tract Infections Management
- Antimicrobial Resistance in Staphylococcus
- Immunodeficiency and Autoimmune Disorders
- Medical Research and Treatments
- Glycosylation and Glycoproteins Research
- Innovative Educational Techniques
- Microbial Natural Products and Biosynthesis
- Carbohydrate Chemistry and Synthesis
University of Taipei
2024
Zhejiang University
2011-2024
Jiangnan University
2008-2024
First Affiliated Hospital Zhejiang University
2019-2024
Bentley University
2023
Xi'an University of Science and Technology
2022
Anhui Polytechnic University
2018-2022
Jingdezhen University
2019
Yunnan University of Finance And Economics
2019
Chinese Center For Disease Control and Prevention
2013
Microbial synthesis of valuable hemoproteins has become a popular research topic, and Pichia pastoris is versatile platform for the industrial production recombinant proteins. However, inadequate supply heme limits high-active hemoproteins. Here strategy enhancing intracellular biosynthesis to improve titers functional activities reported. After selecting suitable expressional globins, efficient heme-supply P. chassis established by removing spatial segregation during biosynthesis,...
To improve the screening efficiency of high-yield neomycin sulfate (NM) Streptomyces fradiae strains after mutagenesis, a high-throughput method using streptomycin resistance prescreening (8 μg/mL) and 24-deep well plates/microplate reader (trypan blue spectrophotometry) rescreening strategy was developed. Using this approach, we identified high-producing NM mutant strain, Sf6-2, via six rounds atmospheric room temperature plasma (ARTP) mutagenesis screening. The displayed potency 7780 ± 110...
BackgroundNanopore sequencing, known for real-time analysis, shows promise rapid clinical infection diagnosis but lacks effective assays bloodstream infections (BSIs).MethodsWe prospectively assessed the performance of a novel nanopore targeted sequencing (NTS) assay in identifying pathogens and predicting antibiotic resistance BSIs, analyzing 387 blood samples from December 2021 to April 2023.ResultsThe positivity rate NTS (69.5%, 269/387) nearly matches that metagenomic next-generation...
Background Plesiomonas shigelloides can cause gastroenteritis and extra-intestinal diseases in humans. However, the prevalence of P. infections has not been investigated China. Methods Consecutive fecal specimens from outpatients with acute diarrhea non-diarrheal patients at nine sentinel hospitals southeast China were collected March 2010 to May 2012. Bacterial pathogens detected by culture, isolates subjected antimicrobial susceptibility testing. We also retrospectively reviewed hospital...
Acute diarrhea is a leading cause of childhood morbidity and mortality worldwide, but there have been few reports on the causative viruses associated with acute among outpatient children in developing countries. This study was conducted to identify viral agents southeastern China.Eight hundred eleven 5 years or younger were enrolled. Five enteric determined by enzyme-linked immunosorbent assay multiplex reverse transcription-polymerase chain reaction for each stool specimen.At least 1 virus...
Ganoderic acids (GAs), derived from the medicinal mushroom Ganoderma lucidum, possess anticancer and other important pharmacological activities. To improve production of GAs, a homologous farnesyl diphosphate synthase (FPS) gene was overexpressed in G. lucidum. Moreover, influence FPS overexpression on GA investigated by developing corresponding mathematical models.The maximum levels total GAs individual (GA-T, GA-S, GA-Me) transgenic strain were 2.76 mg/100 mg dry weight (DW), 41 ± 2, 21 5,...
Diarrhea is the second most common cause of death among children less than 5 years age worldwide. The etiological agents diarrhea in southeast coastal area China were studied from July 2009 to December 2014.A total 2318 patients enrolled this study and examined for presence viruses, bacteria, parasites. Multiplex real-time PCR was used detection diarrheagenic Escherichia.coli (DEC). DEC strains tested susceptibility a panel 20 antibiotics using Kirby-Bauer disc-diffusion method.Of with...
Acute diarrhea is an important public health issue. Here, we focused on the differences of enteropathogens in acute between urban and rural areas southeast China. Laboratory- sentinel-based surveillance (≥ 3 loose or liquid stools/24 hours) was conducted at 16 hospitals. Fecal specimens were tested for bacterial (Aeromonas sp., Campylobacter diarrheagenic Escherichia coli, Plesiomonas shigelloides, non-typhoidal Salmonella, Shigella Vibrio Yersinia sp.) viral (adenovirus, astrovirus,...
<title>Abstract</title> Background Nanopore sequencing, known for real-time analysis, shows promise rapid clinical infection diagnosis but lacks effective assays bloodstream infections (BSIs). Methods We prospectively assessed a novel nanopore targeted sequencing (NTS) assay's performance in identifying pathogens and predicting antibiotic resistance BSIs, analyzing 387 blood samples from December 2021 to April 2023. Results The positivity rate NTS (69.5%, 269/387) nearly matches that of...
In China, V. parahaemolyticus has been a leading cause of foodborne outbreaks and bacterial infectious diarrhea since the 1990s, most infections have associated with pandemic O3:K6 its serovariants. However, comprehensive overview sero-prevalence genetic diversity clone in China is lacking. To compensate for this deficiency, isolates both clinical environmental Chinese samples collected from multiple studies were analyzed study. Surprisingly, as many 27 serovariants identified widely...
Analysis of the genotypic characteristics and antimicrobial susceptibility patterns methicillin-resistant Staphylococcus aureus (MRSA) is essential for control treatment diseases caused by this important pathogen. In study, MRSA isolates obtained from a tertiary caret hospital in China were subjected to spa typing, SCCmec multiple locus sequence typing (MLST), PCR targeting genes encoding Panton-Valentine leukocidin (PVL). The disk diffusion method was used test 10 non-beta-lactam...
Metagenomic next-generation sequencing (mNGS) provides innovative solutions for predicting complex infections. A comprehensive understanding of its strengths and limitations in real-world clinical settings is necessary to ensure that it not overused or misinterpreted.
Enterovirus 71 (EV71) is a major causative agent of hand, foot, and mouth disease (HFMD) in children. The role T follicular helper (TFH) cells EV71-infected children remains unclear regulating humoral immunity. frequency circulating ICO<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:msup><mml:mrow><mml:mtext>S</mml:mtext></mml:mrow><mml:mrow><mml:mtext>high</mml:mtext></mml:mrow></mml:msup></mml:mrow></mml:math>/PD-<mml:math...
Escherichia coli is a leading cause of bloodstream infection (BSI) in hospitals and communities.We conducted retrospective study 2015 to evaluate the clinical features microbiological characteristics E. BSI acquired hospital community.A total 100 patients with were enrolled, among whom 60 % had hospital-onset (HO) while 40 community-onset (CO) BSI. Patients HO higher percentages haematological disorders, immunosuppression conditions, underwent surgery within 2 weeks 30-day mortality. The...
// Dawei Cui 1,2,* , Fengyun Zhong 3,* Jie Lin 4,* Yidong Wu 5 Quan Long 1,2 Xianzhi Yang Qiaoyun Zhu Li Huang Qifen Mao Zhaoxia Huo Zhe Zhou Guoliang Xie Shufa Zheng Fei Yu and Chen 1 Department of Laboratory Medicine, First Affiliated Hospital, College Zhejiang University, Hangzhou, China 2 Key Clinical In Vitro Diagnostic Techniques Province, 3 General Surgery, the Second Hospital Soochow Suzhou, 4 Laboratory, Center Community Health Service Qingbo Street, Hangzhou Children's * These...
Vibrio parahaemolyticus is a leading foodborne pathogen in southeastern China. In this study, 105 strains of V. were isolated from fresh seafood 2013 and 2014. The serotypes, virulence-associated genes sequence types (STs) these analyzed. 26 defined serotypes identified 69 (65.7%) had untypeable O or K antigen. 8 (7.6%) the gene tdh no strain carried trh gene. 45.7% (48/105) isolates contained all four T3SS1 50% (4/8) tdh+ trh- lacked at least one tested T3SS2α genes. could be categorized...
The outbreak of avian influenza A (H7N9) virus infection, with a high mortality rate, has caused concern worldwide. Although interleukin-17- (IL-17-) secreting CD4+ T (Th17) and CD8+ (Tc17) cells have been proven to play crucial roles in the changes Th17 Tc17 immune responses H7N9 infection remain controversial. In this study, we found that frequencies among human peripheral blood mononuclear (PBMCs) as well IL-17A protein mRNA levels were markedly decreased patients acute infection....