A5029095757- Atrial Fibrillation Management and Outcomes
- Cardiac electrophysiology and arrhythmias
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Cardiac Arrhythmias and Treatments
- Chemical Synthesis and Analysis
- Animal Genetics and Reproduction
- RNA modifications and cancer
- Protein Degradation and Inhibitors
- Genomics and Chromatin Dynamics
- Hepatitis B Virus Studies
- Alzheimer's disease research and treatments
- RNA Interference and Gene Delivery
- Liver Disease Diagnosis and Treatment
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Cardiovascular Disease and Adiposity
- RNA regulation and disease
- interferon and immune responses
- Click Chemistry and Applications
- Peroxisome Proliferator-Activated Receptors
- S100 Proteins and Annexins
- Pluripotent Stem Cells Research
- Monoclonal and Polyclonal Antibodies Research
Montreal Heart Institute
2014-2025
The University of Texas Health Science Center at Houston
2018-2024
University of Illinois Urbana-Champaign
2024
Université de Montréal
2014-2024
McGill University
2014-2024
Chinese Academy of Sciences
2013-2024
Chengdu Third People's Hospital
2024
Lifetech Scientific (China)
2024
Chongqing Medical University
2018-2024
Children's Hospital of Chongqing Medical University
2018-2024
Hypercholesterolemia, the driving force of atherosclerosis, accelerates expansion and mobilization hematopoietic stem progenitor cells (HSPCs). The molecular determinants connecting hypercholesterolemia with hematopoiesis are unclear. Here, we report that a somite-derived prohematopoietic cue, AIBP, orchestrates HSPC emergence from hemogenic endothelium, type specialized endothelium manifesting potential. Mechanistically, AIBP-mediated cholesterol efflux activates endothelial Srebp2, master...
Abstract Introduction We investigated the proteomic profiles of amyloid plaques (APs) from Alzheimer's disease (AD) and age‐matched non‐AD brains APP/PS1 transgenic model mice. Methods APs adjacent control regions were collected fresh‐frozen brain sections using laser capture dissection. Proteins quantitated tag‐labeling coupled high‐throughput mass spectra. Results Over 4000 proteins accurately quantified, more than 40 identified as highly enriched in both AD APs, including apoE, midkine,...
Abstract The molecular basis underlying the interaction between retrotransposable elements (RTEs) and human genome remains poorly understood. Here, we profiled N 6 -methyladenosine (m A) deposition on nascent RNAs in cells by developing a new method MINT-Seq, which revealed that many classes of RTE RNAs, particularly intronic LINE-1s (L1s), are strongly methylated. These m A-marked i ntronic L 1s (MILs) evolutionarily young, sense-oriented to hosting genes, bound dozen RNA binding proteins...
Nonalcoholic steatohepatitis (NASH) represents a severe disease subtype of nonalcoholic fatty liver (NAFLD) that is thought to be highly associated with systemic metabolic abnormalities. It characterized by series substantial damage, including hepatocellular steatosis, inflammation, and fibrosis. The end stage NASH, in some cases, may result cirrhosis carcinoma (HCC). Nowadays large number investigations are actively under way test various therapeutic strategies, emerging oligonucleotide...
A Pd-catalyzed cascade oxidation/sp2 C–H bond acylation of azoarenes was developed in which readily available aryl methanes were used as the situ generated acyl sources. This reaction provides a convenient access to ortho-acyl under mild conditions.
Teleost sex differentiation largely depends on the number of undifferentiated germ cells. Here, we describe generation and characterization a novel transgenic zebrafish line, Tg(piwil1:egfp-UTRnanos3)ihb327Tg, which specifically labels whole lifetime cells, i.e., from primordial cells (PGCs) at shield stage to oogonia early oocytes in ovary spermatogonia, spermatocyte, spermatid testis. By using this carefully observed numbers PGCs embryonic juvenile process The became variable as 1 day...
Abstract Transposable elements (TEs) comprise a large proportion of long non-coding RNAs (lncRNAs). Here, we employed CRISPR to delete short interspersed nuclear element (SINE) in Malat1, cancer-associated lncRNA, investigate its significance cellular physiology. We show that Malat1 with SINE deletion forms diffuse speckles and is frequently translocated the cytoplasm. SINE-deleted cells exhibit an activated unfolded protein response PKR markedly increased DNA damage apoptosis caused by...
Abstract Aims Inflammation plays a role in atrial fibrillation (AF), but classical anti-inflammatory molecules are ineffective. Recent evidence suggests that failure of inflammation-resolution causes persistent inflammatory signalling and novel drug-family called resolvins promotes inflammation-resolution. Right heart disease (RHD) is associated with AF; experimental RHD shows signs inflammatory-pathway activation. Here, we evaluated resolvin-therapy effects on arrhythmogenic remodelling...
Abstract Dynamic combinatorial libraries (DCLs) is a powerful tool for ligand discovery in biomedical research; however, the application of DCLs has been hampered by their low diversity. Recently, concept DNA encoding employed to create DNA‐encoded dynamic (DEDLs); all current DEDLs are limited fragment identification, and challenging process linking required after selection. We report an anchor‐directed DEDL approach that can identify full structures from large‐scale DEDLs. This method also...
Utilizing already existing DNA-encoded libraries (DELs) for the generation of a distinct DEL represents an expedited strategy expanding chemical space. Herein, we leverage unique photoreactivity tetrazoles to synthesize diacylhydrazines on DNA. Widely available carboxylic acids serving as building blocks were employed under mild photomediated reaction conditions, affording diverse DNA-conjugated diacylhydrazines. This methodology also demonstrates robustness in DEL-compatible synthesis and...
In this paper, a facile strategy to develop graphene-based delivery nanosystems for effective drug loading and sustained release was proposed validated. Specifically, biocompatible naphthalene-terminated PEG (NP) anticancer drugs (curcumin or doxorubicin (DOX)) were simultaneously integrated onto oxidized graphene (GO), leading self-assembled, nanosized complexes. It found that the oxidation degree of GO had significant impact on drug-loading efficiency structural stability nanosystems....
Characterizing regulatory effects of genomic variants in plants remains a challenge. Although several tools based on deep-learning models and large-scale chromatin-profiling data have been available to predict elements variant effects, no dedicated or web services reported plants. Here, we present PlantDeepSEA as deep learning-based service multiple tissues six plant species (including four crops). provides two main functions. One is called Variant Effector, which aims the sequence chromatin...
Abstract Background Regulation of gene expression plays an essential role in controlling the phenotypes plants. Brassica napus ( B. ) is important source for vegetable oil world, and seed content trait . Results We perform a comprehensive analysis transcriptional variability seeds at two developmental stages, 20 40 days after flowering (DAF). detect 53,759 53,550 independent quantitative loci (eQTLs) 79,605 76,713 expressed genes DAF, respectively. Among them, local eQTLs are mapped to...
Abstract N6-methyladenosine (m 6 A), one of the most prevalent mRNA modifications in eukaryotes, plays a critical role modulating both biological and pathological processes. However, it is unknown whether mutant p53 neomorphic oncogenic functions exploit dysregulation m A epitranscriptomic networks. Here, we investigate Li-Fraumeni syndrome (LFS)-associated neoplastic transformation driven by iPSC-derived astrocytes, cell-of-origin gliomas. We find that but not wild-type (WT) physically...