A5038177541- Mesenchymal stem cell research
- Zebrafish Biomedical Research Applications
- Hematopoietic Stem Cell Transplantation
- Cancer-related Molecular Pathways
- Cell Image Analysis Techniques
- Mitochondrial Function and Pathology
- Neonatal Respiratory Health Research
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Extracellular vesicles in disease
- AI in cancer detection
- Cancer, Hypoxia, and Metabolism
- Medical Image Segmentation Techniques
- Hippo pathway signaling and YAP/TAZ
- Pluripotent Stem Cells Research
- Erythrocyte Function and Pathophysiology
- Metabolism and Genetic Disorders
- Pregnancy and preeclampsia studies
- Cancer Cells and Metastasis
- Nanoplatforms for cancer theranostics
- Cellular Mechanics and Interactions
- Prenatal Screening and Diagnostics
- Cancer Research and Treatments
- Acute Myeloid Leukemia Research
- Single-cell and spatial transcriptomics
The University of Texas Health Science Center at Houston
2013-2024
Brown Foundation
2013-2024
The University of Texas MD Anderson Cancer Center
2020-2024
University Medical Center of the Johannes Gutenberg University Mainz
2024
Johannes Gutenberg University Mainz
2024
Institute for Molecular Medicine
2020
Dana-Farber/Boston Children's Cancer and Blood Disorders Center
2015
Harvard University
2009-2015
Harvard Stem Cell Institute
2009-2015
Regenerative Medicine Institute
2015
Mechanical stress is pervasive in egress routes of malignancy, yet the intrinsic effects force on tumour cells remain poorly understood. Here, we demonstrate that frictional characteristic flow lymphatics stimulates YAP1 to drive cancer cell migration; whereas intensities fluid wall shear (WSS) typical venous or arterial inhibit taxis. YAP1, but not TAZ, strictly required for WSS-enhanced movement, as blockade TEAD1-4 YAP1-TEAD interaction reduces cellular velocity levels observed without...
Hypercholesterolemia, the driving force of atherosclerosis, accelerates expansion and mobilization hematopoietic stem progenitor cells (HSPCs). The molecular determinants connecting hypercholesterolemia with hematopoiesis are unclear. Here, we report that a somite-derived prohematopoietic cue, AIBP, orchestrates HSPC emergence from hemogenic endothelium, type specialized endothelium manifesting potential. Mechanistically, AIBP-mediated cholesterol efflux activates endothelial Srebp2, master...
Traumatic brain injury (TBI) is soon predicted to become the third leading cause of death and disability worldwide. After primary injury, a complex set secondary injuries develops hours days later with prolonged neuroinflammation playing key role. TBI other inflammatory conditions are currently being treated in preclinical clinical trials by number cellular therapies. Mesenchymal stem cells (MSC) great interest due their widespread usage, safety, relative ease isolate culture. However, there...
Blood flow promotes emergence of definitive hematopoietic stem cells (HSCs) in the developing embryo, yet signals generated by hemodynamic forces that influence potential remain poorly defined. Here we show fluid shear stress endows long-term multilineage engraftment upon early tissues at embryonic day 9.5, an stage not previously described to harbor HSCs. Effects on hematopoiesis are mediated part a cascade downstream wall involves calcium efflux and stimulation prostaglandin E2...
The inactivation of the retinoblastoma ( Rb ) tumor suppressor gene in mice results ectopic proliferation, apoptosis, and impaired differentiation extraembryonic, neural, erythroid lineages, culminating fetal death by embryonic day 15.5 (E15.5). Here we show that specific loss trophoblast stem (TS) cells, but not derivatives, leads to an overexpansion trophoblasts, a disruption placental architecture, E15.5. Despite profound abnormalities, tissues appeared remarkably normal, suggesting full...
It has long been known that loss of the retinoblastoma protein (Rb) perturbs neural differentiation, but underlying mechanism never solved. Rb absence impairs cell cycle exit and triggers death some neurons, so differentiation defects may well be indirect. Indeed, we show abnormalities in both light-evoked electrophysiological responses Rb-deficient retinal cells are rescued when ectopic division apoptosis blocked specifically by deleting E2f transcription factor (E2f) 1. However,...
Mesenchymal stromal cells (MSCs) are believed to mobilize from the bone marrow in response inflammation and injury, yet effects of egress into vasculature on MSC function largely unknown. Here we show that wall shear stress (WSS) typical fluid frictional forces present vascular lumen stimulates antioxidant anti-inflammatory mediators, as well chemokines capable immune cell recruitment. WSS specifically promotes signaling through NFκB-COX2-prostaglandin E
Physical forces associated with tumor growth and drainage alter cancer cell invasiveness metastatic potential. We previously showed that fluid frictional force, or shear stress, typical of lymphatic flow induces YAP1/TAZ activation in prostate cells to promote motility dependent upon YAP1 but not TAZ. Here, we show stress elevates TAZ protein levels promotes nuclear localization. Increased activity drives increased DNA synthesis AMOTL2, ANKRD1, CTGF gene transcription independently YAP1....
Inactivation of the retinoblastoma gene in mouse embryos causes tissue infiltrations into critical sections placenta, which has been shown to affect fetal survivability. Our collaborators cancer genetics are extremely interested examining three dimensional nature these given a stack two light microscopy images. Three sets wildtype and mutant placentas was sectioned serially digitized using commercial scanner. Each individual placenta dataset consisted approximately 1000 images totaling 700...
Lymphatic drainage generates force that induces prostate cancer cell motility via activation of Yes-associated protein (YAP), but whether this response to fluid is conserved across types unclear. Here, we show shear stress corresponding flow in the initial lymphatics modifies taxis breast cancer, whereas some lines use rapid amoeboid migration behavior flow, a separate subset decrease movement. Positive responders displayed transcriptional profiles characteristic an state, which typical...