A5048109872- Zebrafish Biomedical Research Applications
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Pluripotent Stem Cells Research
- Pregnancy and preeclampsia studies
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Gout, Hyperuricemia, Uric Acid
- Hedgehog Signaling Pathway Studies
- Liver Disease Diagnosis and Treatment
- Neonatal Respiratory Health Research
- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- Acute Myeloid Leukemia Research
- Eosinophilic Disorders and Syndromes
- Genetic Associations and Epidemiology
- Immune Cell Function and Interaction
- Biomedical Ethics and Regulation
- Infectious Diseases and Mycology
- Erythrocyte Function and Pathophysiology
- Developmental Biology and Gene Regulation
- Renal and related cancers
- Immune cells in cancer
- Hematological disorders and diagnostics
- Nerve injury and regeneration
- Nuclear Receptors and Signaling
Dana-Farber Cancer Institute
2012-2023
Harvard University
2012-2023
Broad Institute
2021-2023
Massachusetts Institute of Technology
2023
Harvard Stem Cell Institute
2012-2016
Howard Hughes Medical Institute
2011-2016
Boston Children's Hospital
2012-2016
Dana-Farber/Boston Children's Cancer and Blood Disorders Center
2015
Brigham and Women's Hospital
2009-2012
Lexington City Schools
2006
Osteoporosis is caused by an imbalance of osteoclasts and osteoblasts, occurring in close proximity to hematopoietic cells the bone marrow. Recurrent somatic mutations that lead expanded population mutant blood termed clonal hematopoiesis indeterminate potential (CHIP). Analyzing exome sequencing data from UK Biobank, we found CHIP be associated with increased incident osteoporosis diagnoses decreased mineral density. In murine models, hematopoietic-specific Dnmt3a, most commonly mutated...
Gout is a common inflammatory arthritis caused by precipitation of monosodium urate (MSU) crystals in individuals with hyperuricemia. Acute flares are accompanied secretion proinflammatory cytokines, including interleukin-1β (IL-1β). Clonal hematopoiesis indeterminate potential (CHIP) an age-related condition predisposing to hematologic cancers and cardiovascular disease. CHIP associated elevated IL-1β, thus we investigated as risk factor for gout. To test the clinical association between...
Hematopoietic stem cells (HSCs) emerge from aortic endothelium via the endothelial-to-hematopoietic transition (EHT). The molecular mechanisms that initiate and regulate EHT remain poorly understood. Here, we show adenosine signaling regulates hematopoietic progenitor cell (HSPC) development in zebrafish embryos. receptor A2b is expressed vascular before HSPC emergence. Elevated levels increased runx1+/cmyb+ HSPCs dorsal aorta, whereas blocking pathway decreased HSPCs. Knockdown of disrupted...
During development, the hematopoietic lineage transits through hemogenic endothelium, but signaling pathways effecting this transition are incompletely characterized. Although Hedgehog (Hh) pathway is hypothesized to play a role in patterning blood formation, early embryonic lethality of mice lacking Hh precludes such analysis. To determine for we assessed effect altered differentiating mouse ES cells, cultured embryos, and developing zebrafish embryos. In cells yolk sac cultures, addition...
Fluid shear stress promotes the emergence of hematopoietic stem cells (HSCs) in aorta–gonad–mesonephros (AGM) developing mouse embryo. We determined that AGM is enriched for expression targets protein kinase A (PKA)–cAMP response element-binding (CREB), a pathway activated by fluid stress. By analyzing CREB genomic occupancy from chromatin-immunoprecipitation sequencing (ChIP-seq) data, we identified bone morphogenetic (BMP) as potential regulator CREB. chemical modulation PKA–CREB and BMP...
Abstract Chronic liver disease is a major public health burden worldwide. Despite various injury mechanisms, progression of chronic follows common pathway inflammation, and fibrosis. We examined the association between clonal hematopoiesis indeterminate potential (CHIP) in 58,358 individuals from four prospective cohorts with whole exome sequencing data (Framingham Heart Study, Atherosclerosis Risk Communities UK Biobank Mass General Brigham Biobank). CHIP was associated an increased risk...