A5017611756- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- 3D Printing in Biomedical Research
- Neurogenesis and neuroplasticity mechanisms
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- RNA modifications and cancer
- Mesenchymal stem cell research
- Prenatal Screening and Diagnostics
- Single-cell and spatial transcriptomics
- Biomedical Ethics and Regulation
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- MicroRNA in disease regulation
- Nerve injury and regeneration
- Tissue Engineering and Regenerative Medicine
- Reproductive Biology and Fertility
- Research Data Management Practices
- Environmental Monitoring and Data Management
- Genetics, Aging, and Longevity in Model Organisms
- Zebrafish Biomedical Research Applications
- DNA Repair Mechanisms
- Muscle Physiology and Disorders
- Neuroscience and Neural Engineering
Harvard University
2010-2025
Massachusetts Institute of Technology
2023-2025
Broad Institute
2025
Beth Israel Deaconess Medical Center
2025
McGovern Institute for Brain Research
2024
Yale University
2018-2022
University of Oxford
2020-2022
Columbia University
2021-2022
Warneford Hospital
2020-2022
Cornell University
2022
Efficient strategies for precise genome editing in human-induced pluripotent cells (hiPSCs) will enable sophisticated engineering research and clinical purposes. The development of programmable sequence-specific nucleases such as Transcription Activator-Like Effectors Nucleases (TALENs) Cas9-gRNA allows genetic modifications to be made more efficiently at targeted sites interest. However, many opportunities remain optimize these tools enlarge their spheres application. We present several...
Human cleavage-stage embryos frequently acquire chromosomal aneuploidies during mitosis due to unknown mechanisms. Here, we show that S phase at the 1-cell stage shows replication fork stalling, low speed, and DNA synthesis extending into G2 phase. damage foci consistent with collapsed forks, DSBs, incomplete form in an ATR- MRE11-dependent manner, followed by spontaneous chromosome breakage segmental aneuploidies. Entry results breakage, whole errors, micronucleation, fragmentation, poor...
Background and Purpose— Intravascular delivery of neural stem cells (NSCs) after stroke has been limited by the low efficiency transendothelial migration. Vascular cell adhesion molecule-1 is an endothelial molecule known to be upregulated early responsible for firm inflammatory expressing surface integrin, CD49d. We hypothesize that enriching NSCs express CD49d injecting them into carotid artery would improve targeted injured brain. Methods— Mouse were analyzed expression fluorescence...
Polycomb Repressive Complex 2 (PRC2) function and DNA methylation (DNAme) are typically correlated with gene repression. Here, we show that PRC2 is required to maintain expression of maternal microRNAs (miRNAs) long non-coding RNAs (lncRNAs) from the Gtl2-Rian-Mirg locus, which essential for full pluripotency iPSCs. In absence PRC2, entire locus becomes transcriptionally repressed due gain DNAme at intergenic differentially methylated regions (IG-DMRs). Furthermore, demonstrate IG-DMR serves...
During development, the hematopoietic lineage transits through hemogenic endothelium, but signaling pathways effecting this transition are incompletely characterized. Although Hedgehog (Hh) pathway is hypothesized to play a role in patterning blood formation, early embryonic lethality of mice lacking Hh precludes such analysis. To determine for we assessed effect altered differentiating mouse ES cells, cultured embryos, and developing zebrafish embryos. In cells yolk sac cultures, addition...
Alternative splicing is a key mechanism underlying cellular differentiation and driver of complexity in mammalian neuronal tissues. However, understanding which isoforms are differentially used or expressed how this affects remains unclear. Long read sequencing allows full-length transcript recovery quantification, enabling transcript-level analysis alternative processes these change with cell state. Here, we utilise Oxford Nanopore Technologies to produce custom annotation well-studied...
Abstract Endogenous neural stem cells normally reside in their niche, the subventricular zone, uninjured rodent brain. Upon stroke, these become more proliferative and migrate away from zone into surrounding parenchyma. It is not known whether this stroke‐induced behavior due to changes niche or introduction of attractive cues infarct both. A related question how transplanted respond subsequent insults, including exogenous have plasticity injuries after engraftment. We addressed issue by...
Abstract Since the revolutionary discovery of induced pluripotent stem cells (iPSCs) by Shinya Yamanaka, comparison between iPSCs and embryonic (ESCs) has revealed significant differences in their epigenetic states developmental potential. A recent compelling study published Nature Buckberry et al. 1 demonstrated that a transient-naive-treatment (TNT) could facilitate reprogramming improve potential human (hiPSCs). However, characterized bulk hiPSCs instead isolating clonal lines overlooked...
Primordial germ cells (PGCs) share many properties with embryonic stem (ESCs) and innately express several key pluripotency-controlling factors, including OCT4, NANOG, LIN28. Therefore, PGCs may provide a simple efficient model for studying somatic cell reprogramming to induced pluripotent (iPSCs), especially in determining the regulatory mechanisms that fundamentally define pluripotency. Here, we report novel of PGC generate iPSCs via transfection SOX2 OCT4 using integrative lentiviral. We...
Human embryonic germ cells (EGCs) provide a powerful model for identifying molecules involved in the pluripotent state when compared to their progenitors, primordial (PGCs), and other stem cells. Microarray Principal Component Analysis (PCA) reveals first time that human EGCs possess transcription profile distinct from PGCs Validation with qRT-PCR confirms express many pluripotency-associated genes but quantifiable differences (ESCs), induced (IPSCs), embryonal carcinoma (ECCs). Analyses...
Abstract The generation of patient‐specific induced pluripotent stem (iPS) cells provides an invaluable resource for cell therapy, in vitro modeling human disease, and drug screening. To date, most iPS have been generated with integrating retro‐ lenti‐viruses are limited their potential utility because residual transgene expression may alter differentiation or induce malignant transformation. Alternatively, transgene‐free methods using adenovirus protein transduction by low efficiency. This...