A5009827302- Cancer Cells and Metastasis
- Digestive system and related health
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Immune cells in cancer
- Diet and metabolism studies
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Cancer Research and Treatments
- Gut microbiota and health
- Pluripotent Stem Cells Research
- Peroxisome Proliferator-Activated Receptors
- Yersinia bacterium, plague, ectoparasites research
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Cancer, Hypoxia, and Metabolism
- IL-33, ST2, and ILC Pathways
- Immune Response and Inflammation
- Pharmacological Effects of Natural Compounds
- Adipose Tissue and Metabolism
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Endometrial and Cervical Cancer Treatments
Cold Spring Harbor Laboratory
2018-2025
Northwell Health
2024
Boston Children's Hospital
2013-2021
Harvard University
2011-2021
Harvard Stem Cell Institute
2013-2021
Howard Hughes Medical Institute
2016-2021
Dana-Farber Cancer Institute
2013-2021
Koch Institute for Integrative Cancer Research At MIT
2016-2021
Allen Institute
2016-2018
Massachusetts Institute of Technology
2016-2017
Abstract Metabolic reprogramming in cancer and immune cells occurs to support their increasing energy needs biological tissues. Here we propose Single Cell Spatially resolved (scSpaMet) framework for joint protein-metabolite profiling of single male human tissues by incorporating untargeted spatial metabolomics targeted multiplexed protein imaging a pipeline. We utilized the scSpaMet profile cell types metabolomic maps 19507, 31156, 8215 lung cancer, tonsil, endometrium tissues,...
Maintenance of astronaut health during spaceflight will require monitoring and potentially modulating their microbiomes. However, documenting microbial shifts has been difficult due to mission constraints that lead limited sampling profiling. Here we executed a six-month longitudinal study quantify the high-resolution human microbiome response three days in orbit for four individuals. Using paired metagenomics metatranscriptomics alongside single-nuclei immune cell profiling, characterized...
Tumor-draining lymph nodes (TDLNs) are important for tumor antigen–specific T cell generation and effective anticancer immune responses. However, TDLNs often the primary site of metastasis, causing suppression worse outcomes. Through cross-species single-cell RNA-Seq analysis, we identified features defining cancer heterogeneity, plasticity, evasion during breast progression node metastasis (LNM). A subset cells in exhibited elevated MHC class II (MHC-II) gene expression both mice humans....
IL-22 is critical for ameliorating obesity-induced metabolic disorders. However, it unknown where acts to mediate these outcomes. Here we examine the importance of tissue-specific IL-22RA1 signaling in mediating long-term high fat diet (HFD) driven To do so, generated intestinal epithelium-, liver-, and white adipose tissue (WAT)-specific Il22ra1 knockout littermate control mice. Intestinal epithelium- liver-specific upregulated systemic glucose metabolism. also mediated liver WAT metabolism...
Polycomb Repressive Complex 2 (PRC2) function and DNA methylation (DNAme) are typically correlated with gene repression. Here, we show that PRC2 is required to maintain expression of maternal microRNAs (miRNAs) long non-coding RNAs (lncRNAs) from the Gtl2-Rian-Mirg locus, which essential for full pluripotency iPSCs. In absence PRC2, entire locus becomes transcriptionally repressed due gain DNAme at intergenic differentially methylated regions (IG-DMRs). Furthermore, demonstrate IG-DMR serves...
Interleukin-22 (IL-22) signaling in the intestines is critical for promoting tissue-protective functions. However, since a diverse array of cell types (absorptive and secretory epithelium as well stem cells) express IL-22Ra1, receptor IL-22, it has been difficult to determine what type(s) specifically respond IL-22 mediate intestinal mucosal host defense. Here, we report that small intestine positively correlated with Paneth differentiation programs. Our...
Endometrial cancer is the most common gynecologic malignancy in United States and one of few malignancies that had an increasing incidence mortality rate over last 10 years. Current research models fail to recapitulate actual characteristics tumor are necessary for proper understanding treatment this heterogenous disease. Patient-derived organoids provide a durable versatile culture system can capture patient-specific such as mutational profile response therapy primary tumor. Here we...
Peroxisome proliferator-activated receptor delta (PPAR-δ) is a nuclear transcription factor that regulates gene expression during development and disease states, such as cancer. However, the precise role of PPAR-δ tumorigenesis not well understood. Recent data suggest may have context-specific oncogenic tumor-suppressive roles depending on tissue, cell-type, or diet-induced physiology in question. For example, intestine, pro-obesity diets, high-fat diet (HFD), are associated with increased...
Background & AimsThe colonic epithelium requires continuous renewal by crypt resident intestinal stem cells (ISCs) and transit-amplifying (TA) to maintain barrier integrity, especially after inflammatory damage. The diet of high-income countries contains increasing amounts sugar, such as sucrose. ISCs TA are sensitive dietary metabolites, but whether excess sugar affects their function directly is unknown.MethodsHere, we used a combination 3-dimensional colonoids mouse model colon...