A5077448525- RNA Research and Splicing
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Viral Infections and Immunology Research
- Cytokine Signaling Pathways and Interactions
- RNA modifications and cancer
- Endometriosis Research and Treatment
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- MicroRNA in disease regulation
- Multiple Myeloma Research and Treatments
- PI3K/AKT/mTOR signaling in cancer
- Protein Kinase Regulation and GTPase Signaling
- Cell death mechanisms and regulation
- Cancer Mechanisms and Therapy
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Phagocytosis and Immune Regulation
- Cancer-related gene regulation
- Reproductive System and Pregnancy
- DNA Repair Mechanisms
- Glutathione Transferases and Polymorphisms
- Ubiquitin and proteasome pathways
- Acute Myeloid Leukemia Research
- Wnt/β-catenin signaling in development and cancer
- Erythrocyte Function and Pathophysiology
- Phytochemical compounds biological activities
Harvard University
2015-2018
Massachusetts General Hospital
2018
Boston Children's Hospital
2013-2017
Goethe University Frankfurt
2008-2017
Dana-Farber Cancer Institute
2013-2016
Harvard Stem Cell Institute
2013
University of Münster
2007-2012
Centre for Cellular and Molecular Biology
2004-2005
Wellcome Trust
2005
Heterozygous somatic mutations in spliceosome genes (U2AF1, SF3B1, ZRSR2, or SRSF2) occur >50% of patients with myelodysplastic syndrome (MDS). These early disease development, suggesting that they contribute to MDS pathogenesis and may represent a unique genetic vulnerability for targeted therapy. Here, we show RNA splicing perturbation by expression the U2AF1(S34F) mutant causes accumulation R loops, transcription intermediate containing RNA:DNA hybrids displaced single-stranded DNA,...
BACKGROUND: Vascular endothelial growth factor (VEGF), a major mediator of angiogenesis and vascular permeability, is known to play key role in the pathophysiology endometriosis. METHODS AND RESULTS: The single nucleotide polymorphisms, −460C>T +405G>C, 5′-untranslated region VEGF gene were tested for association case–control study 215 affected women 210 with no evidence disease. All South Indian origin ascertained from same infertility clinic. genotype allele frequencies polymorphism did...
Objective(s) Studies on association between endometriosis and various phase I II detoxification genes such as glutathione S-transferase M1 theta 1 (GSTM1 GSTT1) cytochrome P450 (CYP1A1) have produced inconsistent results possibly because of ethnic differences. The present study was undertaken to investigate the frequency CYP1A1 (6235T>C) polymorphism GSTM1, GSTT1 null mutations in a South Indian women's population with without endometriosis. Methods frequencies variants were studied 310...
Treatment with tyrosine kinase inhibitors is the standard of care for Philadelphia chromosome positive leukemias. However eradication leukemia initiating cells remains a challenge. Circumstantial evidence suggests that cytokine microenvironment may play role in BCR-ABL mediated leukemogenesis and imatinib resistance. Gene expression analyses ALL long-term cultured revealed strong reduction SOCS mRNA after treatment, thereby demonstrating inhibition signaling. In this study we employed...
<div>Abstract<p>Heterozygous somatic mutations in spliceosome genes (<i>U2AF1, SF3B1, ZRSR2</i>, or <i>SRSF2</i>) occur >50% of patients with myelodysplastic syndrome (MDS). These early disease development, suggesting that they contribute to MDS pathogenesis and may represent a unique genetic vulnerability for targeted therapy. Here, we show RNA splicing perturbation by expression the U2AF1(S34F) mutant causes accumulation R loops, transcription...
<p>Combination of RNA splicing modulator compounds and ATR inhibition sensitizes U2AF1(S34F)-expressing cells.</p>
<p>List of primers used for RNA splicing assay</p>
<p>Protein expression levels of R loop regulators in U2AF1(WT)- or U2AF1(S34F)-expressing cells.</p>
<p>RNA splicing perturbation in cells confers sensitivity to ATR inhibitors.</p>
<p>Immunofluorescence staining of gH2AX DNA damage marker in human CD34+ hematopoietic progenitor cells.</p>
<p>U2AF1(S34F)-expressing cells induce R loop-associated ATR-mediated RPA32 phosphorylation.</p>
<p>RNA splicing perturbation by pharmacologic compounds induce R loop and ATR-mediated RPA32 phosphorylation.</p>
<p>Expression of wild type or different mutants U2AF1.</p>
<p>List of primers used for RNA splicing assay</p>
<p>U2AF1(S34F)-expressing cells induce R loop-associated ATR-mediated RPA32 phosphorylation.</p>
<p>Expression of wild type or different mutants U2AF1.</p>