A5021729143- RNA Research and Splicing
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- RNA modifications and cancer
- Viral Infections and Immunology Research
- MicroRNA in disease regulation
- Acute Myeloid Leukemia Research
- Multi-Criteria Decision Making
- Supply Chain Resilience and Risk Management
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Hepatitis C virus research
- Cellular Mechanics and Interactions
- Influenza Virus Research Studies
- Economic and Technological Innovation
- Bacteriophages and microbial interactions
- Microtubule and mitosis dynamics
- Gene expression and cancer classification
- Quality and Supply Management
- Molecular Biology Techniques and Applications
- Multiple Myeloma Research and Treatments
- Supply Chain and Inventory Management
- Research on Leishmaniasis Studies
- Optimization and Mathematical Programming
- T-cell and B-cell Immunology
- Genomics and Chromatin Dynamics
University of Technology Malaysia
2022-2025
Massachusetts General Hospital
2017-2018
Harvard University
2018
Johns Hopkins University
2012-2015
Johns Hopkins Medicine
2012-2013
Genome Institute of Singapore
2008
Heterozygous somatic mutations in spliceosome genes (U2AF1, SF3B1, ZRSR2, or SRSF2) occur >50% of patients with myelodysplastic syndrome (MDS). These early disease development, suggesting that they contribute to MDS pathogenesis and may represent a unique genetic vulnerability for targeted therapy. Here, we show RNA splicing perturbation by expression the U2AF1(S34F) mutant causes accumulation R loops, transcription intermediate containing RNA:DNA hybrids displaced single-stranded DNA,...
Unexpected worldwide disruptions brought various challenges to supply chain management thus manipulating the research direction towards resilience. Since supplier is one of important elements, can be overcome through resilient selection. Supplier selection a multi-criteria decision-making problem where several criteria are involved. In this study, GRA-BWM-TOPSIS was proposed evaluate suppliers. Seven resilience which were Quality, Lead Time, Cost, Flexibility, Visibility, Responsiveness and...
Aggressive forms of cancer are often defined by recurrent chromosomal alterations, yet in most cases, the causal or contributing genetic components remain poorly understood. Here, we utilized microarray informatics to identify candidate oncogenes potentially aggressive breast behavior. We identified Rab-coupling protein RCP (also known as RAB11FIP1), which is located at a region frequently amplified (8p11–12) potential candidate. Overexpression MCF10A normal human mammary epithelial cells...
Purpose: The intricate interplay between economic dynamism and social cohesion is essential for holistic advancement enduring societal welfare. This systematic review aims to critically evaluate APP literature with a focus on aspects. Previous studies have primarily focused only the aspects, often overlooking leading poor performance profit drop.Design/methodology/approach: We reviewed most recent papers published in last 13 years from 2010 2023 systematically classified them based model...
Interferon alpha (IFNalpha) is a type I interferon that plays major role in host defense. There are 13 different IFNalpha genes humans, but much of the work concerning their viral defense has been limited to studying either subtype 2 or pan due inability distinguish between highly similar genetic and amino acid sequences. Because recent advances molecular biochemical techniques, it possible study regulation individual subtypes. It reported HIV/SIV infection results impaired responses certain...
Abstract Background Pathogen detection using DNA microarrays has the potential to become a fast and comprehensive diagnostics tool. However, since pathogen chips currently utilize random primers rather than specific for RT-PCR step, bias inherent in PCR amplification becomes serious problem that causes large inaccuracies hybridization signals. Results In this paper, we study how efficiency of affects We describe model predicts given primer on target viral genome. The prediction allows us...
Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Twitter Facebook Reddit LinkedIn Tools Reprints and Permissions Cite Search Site Citation Wan Yee Leong, Kuan Yew Wong; on resilient supplier selection. AIP Conf. Proc. 21 March 2024; 2750 (1): 040066. https://doi.org/10.1063/5.0148817 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Dropdown Menu input auto suggest filter...
<div>Abstract<p>Heterozygous somatic mutations in spliceosome genes (<i>U2AF1, SF3B1, ZRSR2</i>, or <i>SRSF2</i>) occur >50% of patients with myelodysplastic syndrome (MDS). These early disease development, suggesting that they contribute to MDS pathogenesis and may represent a unique genetic vulnerability for targeted therapy. Here, we show RNA splicing perturbation by expression the U2AF1(S34F) mutant causes accumulation R loops, transcription...
<p>Combination of RNA splicing modulator compounds and ATR inhibition sensitizes U2AF1(S34F)-expressing cells.</p>
<p>List of primers used for RNA splicing assay</p>
<p>Protein expression levels of R loop regulators in U2AF1(WT)- or U2AF1(S34F)-expressing cells.</p>
<p>RNA splicing perturbation in cells confers sensitivity to ATR inhibitors.</p>
<p>Immunofluorescence staining of gH2AX DNA damage marker in human CD34+ hematopoietic progenitor cells.</p>
<p>U2AF1(S34F)-expressing cells induce R loop-associated ATR-mediated RPA32 phosphorylation.</p>
<p>RNA splicing perturbation by pharmacologic compounds induce R loop and ATR-mediated RPA32 phosphorylation.</p>
<p>Expression of wild type or different mutants U2AF1.</p>
<p>List of primers used for RNA splicing assay</p>
<p>U2AF1(S34F)-expressing cells induce R loop-associated ATR-mediated RPA32 phosphorylation.</p>
<p>Expression of wild type or different mutants U2AF1.</p>
<p>RNA splicing perturbation in cells confers sensitivity to ATR inhibitors.</p>
<p>Protein expression levels of R loop regulators in U2AF1(WT)- or U2AF1(S34F)-expressing cells.</p>