A5008334875- Zebrafish Biomedical Research Applications
- Pregnancy and preeclampsia studies
- Epigenetics and DNA Methylation
- Reproductive System and Pregnancy
- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- Birth, Development, and Health
- Genetics and Neurodevelopmental Disorders
- Single-cell and spatial transcriptomics
- Genetic Syndromes and Imprinting
- Cancer Risks and Factors
- Tissue Engineering and Regenerative Medicine
- Circular RNAs in diseases
- Ectopic Pregnancy Diagnosis and Management
- Neonatal Respiratory Health Research
- Erythrocyte Function and Pathophysiology
- Developmental Biology and Gene Regulation
- Acute Myeloid Leukemia Research
- Congenital heart defects research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- T-cell and B-cell Immunology
- Electrospun Nanofibers in Biomedical Applications
McGill University
2021-2022
The University of Texas Health Science Center at Houston
2018-2021
Institute for Molecular Medicine
2021
Fundação Oswaldo Cruz
2013-2019
Queen's University
2016
It is widely believed that hematopoiesis after birth established by hematopoietic stem cells (HSCs) in the bone marrow and HSC-independent limited only to primitive erythro-myeloid tissue-resident innate immune arising embryo. Here, surprisingly, we find significant percentages of lymphocytes are not derived from HSCs, even 1-year-old mice. Instead, multiple waves occur embryonic day 7.5 (E7.5) E11.5 endothelial cells, which simultaneously produce HSCs lymphoid progenitors constitute many...
Abstract Background DNA methylation plays an important role in regulating gene expression mammals. The covalent DNMT1 inhibitors 5-azacytidine and decitabine are widely used research to reduce levels, but they impart severe cytotoxicity which limits their demethylation capability confounds interpretation of experiments. Recently, a non-covalent inhibitor called GSK-3484862 was developed by GlaxoSmithKline. We sought determine whether can induce more effectively than 5-azanucleosides. Murine...
Mice ablated for the gene encoding transcription factor Nfil3 lack peripheral natural killer (NK) cells but retain tissue-resident NK cells, particularly in mucosal sites, including virgin uterus. We undertook a time course histological study of implantation sites from syngeneically (Nfil3(-/-)) and allogeneically (BALB/c) mated Nfil3(-/-) females. also examined Rag2(-/-)Il2rg(-/-) females preconditioned by adoptive transfer marrow or uterine cell suspensions to identify pregnancy...
Previous studies have shown that human and mouse placentas hematopoietic potential during mid-gestation. In this investigation, we used histological immunohistological approaches to visualize cells in placenta between 9.5 12.5 days of gestation (gd), identifying their topography niche. Putative foci were present on 10.5 11.5 gd but not or was restricted the placental labyrinth. Two major niches each with distinctive cell clusters present. One type cluster involved chorioallantoic vasculature...
B1 lymphocytes are a small but unique component of the innate immune-like cells. However, their ontogenic origin is still matter debate. Although it widely accepted that cells originate early in fetal life, whether or not they arise from hematopoietic stem (HSCs) unclear. In order to shed light on cell origin, we set out determine lineage specification dependent Notch signaling, which essential for HSC generation and, therefore, all derivatives lineages. Using mouse embryonic (mESCs)...
Summary The stem cell theory that all blood cells are derived from hematopoietic (HSC) is a central dogma in hematology. However, various types of already produced hemogenic endothelial (HECs) before the first HSCs appear at embryonic day (E)11 mouse embryo. This early production HECs, called HSC-independent hematopoiesis, includes primitive and definitive erythromyeloid progenitors transiently support fetal homeostasis until HSC-derived hematopoiesis established. Lymphoid potential has...
Abstract Background DNA methylation plays an important role in regulating gene expression mammals. The covalent DNMT1 inhibitors 5-azacytidine and decitabine are widely used research to reduce levels, but they impart severe cytotoxicity which limits their demethylation capability confounds interpretation of experiments. Recently, a non-covalent inhibitor called GSK-3484862 was developed by GlaxoSmithKline. We sought determine whether can induce more effectively than 5-azanucleosides. Murine...