A5057549513- Adipose Tissue and Metabolism
- Advanced Proteomics Techniques and Applications
- Zebrafish Biomedical Research Applications
- Metabolomics and Mass Spectrometry Studies
- Immune cells in cancer
- Bioinformatics and Genomic Networks
- Pregnancy and preeclampsia studies
- Biotin and Related Studies
- T-cell and B-cell Immunology
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- Acute Myeloid Leukemia Research
- Neonatal Respiratory Health Research
- Immune Cell Function and Interaction
Japan Lifeline (Japan)
2024
Yokohama City University
2023-2024
University of Tsukuba
2023-2024
RIKEN Center for Integrative Medical Sciences
2023-2024
RIKEN Center for Advanced Intelligence Project
2021-2024
Tertiary lymphoid tissues (TLTs) facilitate local T and B cell interactions in chronically inflamed organs. However, the cells molecular pathways that govern TLT formation are poorly defined. Here, we identified TNF superfamily CD153/CD30 signaling between 2 unique age-dependent lymphocyte subpopulations, CD153+PD-1+CD4+ senescence-associated (SAT) CD30+T-bet+ age-associated (ABCs), as a driver for expansion. SAT cells, which produced ABC-inducing factors IL-21 IFN-γ, ABCs progressively...
It is widely believed that hematopoiesis after birth established by hematopoietic stem cells (HSCs) in the bone marrow and HSC-independent limited only to primitive erythro-myeloid tissue-resident innate immune arising embryo. Here, surprisingly, we find significant percentages of lymphocytes are not derived from HSCs, even 1-year-old mice. Instead, multiple waves occur embryonic day 7.5 (E7.5) E11.5 endothelial cells, which simultaneously produce HSCs lymphoid progenitors constitute many...
Abstract Mapping protein interaction complexes in their natural state vivo is arguably the Holy Grail of network analysis. Detection stoichiometry has been an important technical challenge, as few studies have focused on this. This may, however, be solved by artificial intelligence (AI) and proteomics. Here, we describe development HaloTag-based affinity purification mass spectrometry (HaloMS), a high-throughput HaloMS assay for discovery. The approach enables rapid capture newly expressed...
Summary The stem cell theory that all blood cells are derived from hematopoietic (HSC) is a central dogma in hematology. However, various types of already produced hemogenic endothelial (HECs) before the first HSCs appear at embryonic day (E)11 mouse embryo. This early production HECs, called HSC-independent hematopoiesis, includes primitive and definitive erythromyeloid progenitors transiently support fetal homeostasis until HSC-derived hematopoiesis established. Lymphoid potential has...
ABSTRACT Mapping protein interaction complexes in their natural state vivo represents the holy grail of network analysis. Detection stoichiometry has been an important technical challenge, as few studies have focused this, yet this may be solved by artificial intelligence and proteomics. Here, we describe development HaloMS, a high-throughput HaloTag-based affinity purification–mass spectrometry assay for discovery. The approach enables rapid capture newly expressed proteins, eliminating...