A5100699051- Pluripotent Stem Cells Research
- Congenital heart defects research
- Angiogenesis and VEGF in Cancer
- Epigenetics and DNA Methylation
- Zebrafish Biomedical Research Applications
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Renal and related cancers
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Multilevel Inverters and Converters
- Cancer, Hypoxia, and Metabolism
- melanin and skin pigmentation
- Animal Genetics and Reproduction
- Nuclear Structure and Function
- Circular RNAs in diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Cell Image Analysis Techniques
- Lipid metabolism and disorders
- Bone Metabolism and Diseases
- Advanced MRI Techniques and Applications
Northwestern Polytechnical University
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2015-2024
Second Hospital of Tianjin Medical University
2024
Institute of Hematology & Blood Diseases Hospital
2024
Tianjin Medical University General Hospital
2024
Tianjin Medical University
2024
Houston Methodist
2014-2023
Institute of Biophysics
2011-2020
Chinese Academy of Sciences
2011-2020
Peking Union Medical College Hospital
2019
Abstract The rapid generation of various species and strains laboratory animals using CRISPR/Cas9 technology has dramatically accelerated the interrogation gene function in vivo . So far, dominant approach for genotyping genome-modified been T7E1 endonuclease cleavage assay. Here, we present a polyacrylamide gel electrophoresis-based (PAGE) method to genotype mice harboring different types indel mutations. We developed 6 system utilized this from F0 F2 generation, which included single...
The role of receptors for endogenous metabolic danger signals-associated molecular patterns has been characterized recently as bridging innate immune sensory systems to initiation inflammation in bone marrow-derived cells, such macrophages. However, it remains unknown whether endothelial cells (ECs), the cell type with largest numbers and first vessel exposed circulating blood, can sense hyperlipidemia. This report determined caspase-1 plays a ECs sensing hyperlipidemia promoting EC...
During sepsis, acute lung injury (ALI) results from activation of innate immune cells and endothelial by endotoxins, leading to systemic inflammation through proinflammatory cytokine overproduction, oxidative stress, intracellular Ca2+ overload. Despite considerable investigation, the underlying molecular mechanism(s) LPS-induced ALI remain elusive. To determine whether stromal interaction molecule 1–dependent (STIM1-dependent) signaling drives dysfunction in response LPS, we investigated...
Background— Cell fate is fluid and may be altered experimentally by the forced expression of master regulators mediating cell lineage. Such reprogramming has been achieved with use viral vectors encoding transcription factors. We recently discovered that are more than passive vehicles for factors because they participate actively in process nuclear to pluripotency increasing epigenetic plasticity. On basis this recognition, we hypothesized small-molecule activators toll-like receptor 3,...
Efficient nuclear reprogramming of somatic cells to pluripotency requires activation innate immunity. Because immune triggers reactive oxygen species (ROS) signaling, we sought determine whether there was a role ROS signaling in reprogramming. We examined production during the doxycycline (dox)-inducible mouse embryonic fibroblasts (MEFs) carrying Yamanaka factors (Oct4, Sox2, Klf4, and c-Myc [OSKM]) into induced pluripotent stem (iPSCs). generation substantially increased with onset...
Hypercholesterolemia, the driving force of atherosclerosis, accelerates expansion and mobilization hematopoietic stem progenitor cells (HSPCs). The molecular determinants connecting hypercholesterolemia with hematopoiesis are unclear. Here, we report that a somite-derived prohematopoietic cue, AIBP, orchestrates HSPC emergence from hemogenic endothelium, type specialized endothelium manifesting potential. Mechanistically, AIBP-mediated cholesterol efflux activates endothelial Srebp2, master...
To investigate the dysregulation of microRNAs (miRNAs) during differentiation osteoclasts and precise roles miR-125a-5p in osteoclasts.The cell model RAW 264.7 osteoclast precursor induced by RANKL plus M-CSF stimulation was established. During early stage differentiation, miRNA expression profiles were detected using biochip technique analyzed cluster analysis. TargetScan, miRTarBase miRDB database analysis applied to find key target genes miR-125a-5p. A dual luciferase experiment conducted...
Human genetic studies identified a strong association between loss of function mutations in RBFOX2 and hypoplastic left heart syndrome (HLHS). There are currently no Rbfox2 mouse models that recapitulate HLHS. Therefore, it is still unknown how as an RNA binding protein contributes to development. To address this, we conditionally deleted embryonic hearts found profound defects cardiac chamber yolk sac vasculature formation. Importantly, our conditional knockout model recapitulated several...
A comprehensive understanding of endothelial cell lineage specification will advance cardiovascular regenerative medicine. Recent studies found that unique epigenetic signatures preferentially regulate identity genes. We thus systematically investigate the landscape and identify MECOM to be leading candidate as an regulator. Single-cell RNA-Seq analysis verifies MECOM-positive cells are exclusively enriched in cluster bona fide derived from induced pluripotent stem cells. Our experiments...
Angiogenesis improves perfusion to the ischemic tissue after acute vascular obstruction. in pathophysiological settings reactivates signaling pathways involved developmental angiogenesis. We showed previously that AIBP (apolipoprotein A-I [apoA-I]-binding protein)-regulated cholesterol efflux endothelial cells controls zebra fish embryonic angiogenesis.This study is determine whether loss of affects angiogenesis mice during development and under pathological conditions explore underlying...
The angiogenic response to ischemia restores perfusion so as preserve tissue. A role for mesenchymal-to-endothelial transition in the is controversial. This study determine if resident fibroblasts contribute angiogenesis.We utilized murine model of hindlimb ischemia, and vivo Matrigel plug assay together with lineage tracing studies single cell RNA-sequencing examine transcriptional functional changes ischemia.Lineage using Fsp1-Cre: R26R-EYFP mice revealed emergence within ischemic a small...
Angiogenesis is integral for embryogenesis, and targeting angiogenesis improves the outcome of many pathological conditions in patients. TBX20 a crucial transcription factor embryonic development, its deficiency associated with congenital heart disease. However, role has not been described.Loss- gain-of-function approaches were used to explore both vitro vivo. gene array was identify key downstream targets TBX20.Unbiased survey showed that knockdown profoundly reduced angiogenesis-associated...
We have previously shown that innate immunity is necessary for transdifferentiation of fibroblasts to endothelial cells. A major signaling molecule involved in inducible nitric oxide synthase (iNOS). Accordingly, we hypothesized iNOS-generated (NO) might enhance transdifferentiation.To elucidate the role NO epigenetic plasticity during transdifferentiation.We exposed BJ formulation included growth factors and immune activator polyinosinic:polycytidylic acid induce Generation...
Abstract Temporal and spatial-specific regulation of pluripotency networks is largely dependent on the precise modifications core transcription factors. Misregulation glutamylation implicated in severe physiological abnormalities. However, how regulates cell reprogramming remains elusive. Here we show that cytosolic carboxypeptidases 1 (CCP1) or CCP6 deficiency substantially promotes induced pluripotent (iPSC) induction embryonic stem cells (ESCs). Klf4 polyglutamylation at Glu381 by tubulin...
Angiomotin (Amot), the founding member of Motin family, is involved in angiogenesis by regulating endothelial cell motility, and required for visceral endoderm movement mice. However, little known about biological functions other two members Angiomotin-like1 (Amotl1) Angiomotin-like2 (Amotl2). Here, we have identified zebrafish amotl2 as an Fgf-responsive gene. Zebrafish expressed maternally restricted types zygotically. Knockdown expression delays epiboly impairs convergence extension...
Abstract Conversion between cell types, e.g., by induced expression of master transcription factors, holds great promise for cellular therapy. Our ability to manipulate identity is constrained incomplete information on genes (CIGs) and their regulation. Here, we develop CEFCIG, an artificial intelligent framework uncover CIGs further define regulators. On the basis machine learning, CEFCIG reveals unique histone codes transcriptional regulation reported CIGs, utilizes these predict...
Biological processes are controlled by groups of genes acting in concert. Investigating gene-gene interactions within different cell types can help researchers understand the regulatory mechanisms behind human complex diseases, such as tumors. We collected extensive single-cell RNA-seq data from tumors, involving 563 patients with 44 tumor types. Through our analysis, we identified various tumors and created an atlas immune subsets across Using SCINET method, reconstructed interactome...
Children with Hutchinson–Gilford progeria syndrome (HGPS) succumb to myocardial infarction and stroke in their teen years. Endothelial dysfunction is an early event more common forms of atherosclerosis. pathobiology may contribute HGPS, but a comprehensive characterization endothelial function HGPS has not been performed.iPSCs derived from fibroblasts patients or unaffected relatives were differentiated into cells (ECs). Immunofluorescent signal the pluripotent stem cell markers SSEA4, Oct4,...